Smartphone-based portable biosensing system using impedance measurement with printed electrodes for 2,4,6-trinitrotoluene (TNT) detection

Zhang, Diming; Jiang, Jing; Chen, Junye; Zhang, Qian; Lu, Yanli; Yao, Yao; Li, Shuang; Liu, Gang Logan; Liu, Qingjun

doi:10.1016/j.bios.2015.03.004

Cited by 127 publications

(51 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Smartphones are portable, widely available, user-friendly, and therefore well suited to act as an effective platform for on-site detection [27,28]. Many interesting biosensors have been designed based on smartphones utilizing mobile apps and various connected devices, such as high-performance cameras and light sensors [28,29,30,31,32,33,34,35,36]. For example, Wei et al reported a dual-illumination color and dual-cuvette hand-held detection platform that used a smartphone’s camera and image processing app for monitoring mercury contamination in water [37].…”

Section: Introductionmentioning

confidence: 99%

A Portable Smart-Phone Readout Device for the Detection of Mercury Contamination Based on an Aptamer-Assay Nanosensor

Xiao

et al. 2016

Sensors

View full text Add to dashboard Cite

The detection of environmental mercury (Hg) contamination requires complex and expensive instruments and professional technicians. We present a simple, sensitive, and portable Hg2+ detection system based on a smartphone and colorimetric aptamer nanosensor. A smartphone equipped with a light meter app was used to detect, record, and process signals from a smartphone-based microwell reader (MR S-phone), which is composed of a simple light source and a miniaturized assay platform. The colorimetric readout of the aptamer nanosensor is based on a specific interaction between the selected aptamer and Hg2+, which leads to a color change in the reaction solution due to an aggregation of gold nanoparticles (AuNPs). The MR S-phone-based AuNPs-aptamer colorimetric sensor system could reliably detect Hg2+ in both tap water and Pearl River water samples and produced a linear colorimetric readout of Hg2+ concentration in the range of 1 ng/mL–32 ng/mL with a correlation of 0.991, and a limit of detection (LOD) of 0.28 ng/mL for Hg2+. The detection could be quickly completed in only 20 min. Our novel mercury detection assay is simple, rapid, and sensitive, and it provides new strategies for the on-site detection of mercury contamination in any environment.

show abstract

Section: Introductionmentioning

confidence: 99%

A Portable Smart-Phone Readout Device for the Detection of Mercury Contamination Based on an Aptamer-Assay Nanosensor

Xiao

et al. 2016

Sensors

View full text Add to dashboard Cite

show abstract

“…In the conventional design, the electrochemical measurements were often performed by potentiostat detecting circuits, while smartphone was only used as a platform to control and display the measurement. Data communications between smartphone and detecting device were often through wire or wireless ways, such as audio jack, Bluetooth, and NFC (Nemiroski et al, 2014;Kassal et al, 2015;Zhang et al, 2015). Generally, the wire communications had a good universality for different generation of mobile phone, while the wireless communications can provide a better mobility for sensor instruments.…”

Section: Future Perspectivesmentioning

confidence: 98%

Biosensors and bioelectronics on smartphone for portable biochemical detection

Zhang

Liu

2016

Biosensors and Bioelectronics

Self Cite

562

216

View full text Add to dashboard Cite

“…Singh et al 59 Yu et al 60 and Zhang et al 61 developed electro-immuno sensors with IDEs by using rectangular arrays for measuring the presence of Ricin. Authors measured the impedance variation by CV and EIS on the IDEs.…”

Section: Electro-immuno Sensorsmentioning

confidence: 99%

Electro-Immuno Sensors: Current Developments and Future Trends

Barbosa¹,

Segura²,

Osma³

2017

IJBSBE

View full text Add to dashboard Cite

Abbreviations: Iot, internet of things; ELISA, enzyme-linked immuno sorbent assay; WB, western blot; IHC, immunohistochemistry; ICC, immunocytochemistry; FACS, fluorescence-activated cell sorting; IP, immuno precipitation; ELISPOT, enzyme-linked immuno spot; PSA, prostate specific antigen; IDEs, interdigitated electrodes; HPV, human papilloma virus IntroductionBiosensors have been mostly used in the food industry, medical and biological fields, among others, where physical and chemical sensors cannot accurately measure all variables. Biosensors are usually comprised by a biological recognition element and a transducer.1,2 Among the different commercial biosensors, the most common recognition elements are DNA, 3 enzymes, 4 aptamers 5,6 and antibodies.7,8 DNA-based biosensors detect specific analytes by their cleavage and ligation to a functionalized DNA strand.9 Enzymatic biosensors immobilize an enzyme to an electrode to facilitate the transport of electrons from the enzyme to the electrode, produced by specific reactions at the active site of the enzyme.2 Aptamers are chemically synthesized oligonucleotides 10 that provide high specificity and sensitivity towards a specific target. Antibody-based biosensors recognize a specific antigen from the immobilization of a monoclonal or polyclonal antibody.8 In general, transducers vary according to the end user towards which the sensor is thought. Among others, transducers include electrochemical responses, mass changes, optical absorption or transmission, and thermal readings. During the last decades, several immunoassay techniques have appeared as a response to the need of having specific and sensitive tests for antigen recognition. The methods by which the recognition is perform varies from one to another, but the presence of at least one recognition antibody remains as a constant. Among others, the most common immune tests used in laboratories and medical facilities are the enzyme-linked immuno sorbent assay (ELISA), western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), fluorescence-activated cell sorting (FACS), immuno precipitation (IP), and enzyme-linked immuno spot (ELISPOT). Figure 1 shows a general diagram of these immunoassays.ELISA is a widely used technique for the recognition of antibodies' antigens within a sample by means of the biochemical recognition of an enzyme.11 In this technique, specific antibodies are conjugated to an enzyme that catalyzes a colorimetric molecule. Spectrophotometric measurements are used to determine the antigen concentration in the sample. ELISA tests can be performed in a qualitative way, in which the results are positive or negative by statistical methods; or quantitative, in which a standard curve from spectrophotometric measurements can be used to quantify the concentration of the antigen. 12WB is a technique for the detection of specific proteins in a sample through the electrophoretic transfer from a separating gel to a blotting matrix.13 WB uses available monoclonal or polyclonal antibodies for the d...

show abstract

Smartphone-based portable biosensing system using impedance measurement with printed electrodes for 2,4,6-trinitrotoluene (TNT) detection

Cited by 127 publications

References 43 publications

A Portable Smart-Phone Readout Device for the Detection of Mercury Contamination Based on an Aptamer-Assay Nanosensor

A Portable Smart-Phone Readout Device for the Detection of Mercury Contamination Based on an Aptamer-Assay Nanosensor

Biosensors and bioelectronics on smartphone for portable biochemical detection

Electro-Immuno Sensors: Current Developments and Future Trends

Contact Info

Product

Resources

About