Smartphone Optosensing Platform Using a DVD Grating to Detect Neurotoxins

Li-ju, Wang; Chang, Yu‐Chung; Ge, Xiaoxiao; Osmanson, Allison T.; Du, Dan; Lin, Yuehe; Li, Lei

doi:10.1021/acssensors.5b00204

Cited by 59 publications

(30 citation statements)

References 28 publications

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“…In the high concentration range (0.8−5 pmol L −1 ), the regression equation was i pc = −0.0049[paraoxon] −13.615 ( R 2 = 0.9954). Meanwhile, the LOD of Nafion/M‐Cell/CNT@AAIL/GCE biosensor was calculated to be 3 fmol L −1 (S/N = 3), which was 2–8 orders of magnitude lower than those of the various reported paraoxon assay methods, including spectrophotometry, fluorescence, electrochemistry, flow‐injection amperometric assay, and other methods (Table S1, Supporting Information). Therefore, the as‐fabricated biosensor showed ultrasensitivity and short detection time, which was more suitable for on‐site detection of trace level of OPP.…”

Section: Resultsmentioning

confidence: 96%

“…In addition, many electrochemical OPP sensors were applied at positive potential, based on oxidation currents of analytes, where some antioxidants in real samples can also be oxidized. More importantly, the limit‐of‐detection (LOD) values of the reported OPP analytic methods (containing electrochemical and non‐electrochemical methods) generally are within 0.12 pmol L −1 to 200 nmol L −1 . Nevertheless, the OPP residues of persisting in crops and environment may be lower .…”

Section: Introductionmentioning

confidence: 99%

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Rational Integration of Biomineralization, Microbial Surface Display, and Carbon Nanocomposites: Ultrasensitive and Selective Biosensor for Traces of Pesticides

Han

Chen

2018

Adv Materials Inter

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real-time detection, simple operation, high sensitivity, and miniaturization. [12,13] So far, the most of electrochemical OPP biosensors are based on the inhibiting effect of enzymes, particularly acetylcholinesterase (AChE). [14][15][16][17][18][19][20] However, the poor specificity of AChE and enzyme inhibition-based sensing mechanism can make electrochemical biosensors to have low selectivity, complicated analysis process, low sensitivity, and slow response, restricting their application. In addition, many electrochemical OPP sensors were applied at positive potential, based on oxidation currents of analytes, [21,22] where some antioxidants in real samples can also be oxidized. More importantly, the limit-of-detection (LOD) values of the reported OPP analytic methods (containing electrochemical and non-electrochemical methods) generally are within 0.12 pmol L −1 to 200 nmol L −1 . [5,[21][22][23][24][25][26][27][28][29][30][31][32][33][34] Nevertheless, the OPP residues of persisting in crops and environment may be lower. [4,5] Therefore, it is of great significance to fabricate ultrasensitive and efficient OPP biosensor.To overcome these above problems, organophosphate hydrolase (OPH) is introduced into electrochemical OPP biosensors to substitute enzyme inhibition-based biosensors. [35] OPH can specifically hydrolyze organophosphate compounds, where OPP are the substrate of the enzyme rather than the inhibitor. [1,36] Hence, OPH-based analytical methods generally show good selectivity and simple assay procedure. However, there are relatively few reports about the electrochemical biosensor based on OPH, compared with other biosensors. [27,37] In previous work, we prepared OPH-fused cell-cobalt phosphate (CP) bioinorganic hybrid materials, that is, mineralized cell (M-Cell), for enhancing the activity of OPH by the integration of allosteric effect, [38,39] biomineralization technology, [40] and cell surface display technology. [16] However, concomitantly, inorganic salt (CP) and whole-cell can reduce the conductivity and electrochemical activity of M-Cell hybrid catalysts. Therefore, it is interesting and necessary to improve the electrochemical activity of M-Cell hybrid catalysts in order to establish the electrochemical OPP biosensor with ultrasensitivity.As is well known, carbon nanotube (CNT) is extensively used to construct electrochemical biosensors, due to the large specific surface area, good stability, and high conductivity. [41] However, Nowadays, the over misuse of organophosphate pesticides (OPP) has seriously threatened human health and environment, and it is challenging and indispensable to establish biosensor for supersensitive, reliable, and fast detection of traces of OPP in real samples. This work provides an interdisciplinary strategy for the construction of electrochemical OPP biosensor with supersensitivity, high selectivity, simple operation, and fast response. First, amino acid ionic liquid (AAIL) is used as stabilizer to dramatically improve the dispersibility of carbon nanotube (CNT) i...

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Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Rational Integration of Biomineralization, Microbial Surface Display, and Carbon Nanocomposites: Ultrasensitive and Selective Biosensor for Traces of Pesticides

Han

Chen

2018

Adv Materials Inter

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“…In advance, we split and diffracted light from each channel into spectrum using a piece of a digital versatile disc (DVD) with a grating period of 710 nm [36]. The DVD grating was placed right in front of the camera lens of the smartphone.…”

Section: Methodsmentioning

confidence: 99%

An ultra‐low‐cost smartphone octochannel spectrometer for mobile health diagnostics

Li-ju

Naudé

Chang

et al. 2018

Journal of Biophotonics

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With the rapid development and proliferation of mobile devices with powerful computing power and the ability of integrating sensors into mobile devices, the potential impact of mobile health (mHealth) diagnostics on the public health is drawing researchers' attention. We developed a Smartphone Octo-channel Spectrometer (SOS) as a mHealth diagnostic tool. The SOS has nanoscale wavelength resolution, is self-illuminated from the smartphone itself, and is ultra-low cost (less than $20). A user interface controls the optical sensing parameters and precise alignment. After calibrating and testing the SOS by quantifying protein concentrations, we clinically validated the SOS by comparing the diagnostic performance of our device with that of a clinical spectrophotometer. About 180 serum samples from de-identified patients with 4 types of autoantibodies were blindly read the ELISA results. The accuracy of the SOS achieved 100% across the clinical reportable range compared with the FDA-approved instrument. Furthermore, the self-illuminated SOS only requires about half of the light intensity of the FDA-approved instrument to achieve clinical-level sensitivity. The low-energy-consumption and low-cost SOS enables point-of-care spectrophotometric sensing in low-resource areas, and can be integrated into point-of-care diagnostic systems for rapid multiplex readout and analysis at patient bedside or at home.

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“…For DNA sequencing, rapid progress in recent years has led to progressively smaller devices which make sequencing at POC settings steps closer to reality. Currently, the MiniSeq system from Illumina is the smallest of the company’s fleet of sequencers; with dimensions of 18 in × 18.9 in × 20.4 in and weight of 99lbs, it is small enough to fit on top of a laboratory bench 140 . This system features onboard analysis to make it easy-to-use.…”

Section: Recent Developments In Poc Diagnosticsmentioning

confidence: 99%