Smartphone supported backlight illumination and image acquisition for microfluidic-based point-of-care testing

Chen, Gang; Chai, Hui; Yu, Ling; Fang, Can

doi:10.1364/boe.9.004604

Cited by 13 publications

(5 citation statements)

References 22 publications

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“…In addition, the flow rate of the blood sample was specifically set to obtain the blood viscosity using a syringe pump. To resolve the issues regarding a more portable rheometer [ 25 ], the present method will be improved in the near future by adapting a portable imaging acquisition system [ 3 , 50 , 51 , 52 , 53 , 54 ], and by adding passive pumps [ 55 ].…”

Section: Resultsmentioning

confidence: 99%

Assessment of Blood Biophysical Properties Using Pressure Sensing with Micropump and Microfluidic Comparator

Kang

2022

Micromachines

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To identify the biophysical properties of blood samples consistently, macroscopic pumps have been used to maintain constant flow rates in a microfluidic comparator. In this study, the bulk-sized and expensive pump is replaced with a cheap and portable micropump. A specific reference fluid (i.e., glycerin solution [40%]) with a small volume of red blood cell (RBC) (i.e., 1% volume fraction) as fluid tracers is supplied into the microfluidic comparator. An averaged velocity (<Ur>) obtained with micro-particle image velocimetry is converted into the flow rate of reference fluid (Qr) (i.e., Qr = CQ × Ac × <Ur>, Ac: cross-sectional area, CQ = 1.156). Two control variables of the micropump (i.e., frequency: 400 Hz and volt: 150 au) are selected to guarantee a consistent flow rate (i.e., COV < 1%). Simultaneously, the blood sample is supplied into the microfluidic channel under specific flow patterns (i.e., constant, sinusoidal, and periodic on-off fashion). By monitoring the interface in the comparator as well as Qr, three biophysical properties (i.e., viscosity, junction pressure, and pressure-induced work) are obtained using analytical expressions derived with a discrete fluidic circuit model. According to the quantitative comparison results between the present method (i.e., micropump) and the previous method (i.e., syringe pump), the micropump provides consistent results when compared with the syringe pump. Thereafter, representative biophysical properties, including the RBC aggregation, are consistently obtained for specific blood samples prepared with dextran solutions ranging from 0 to 40 mg/mL. In conclusion, the present method could be considered as an effective method for quantifying the physical properties of blood samples, where the reference fluid is supplied with a cheap and portable micropump.

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Section: Resultsmentioning

confidence: 99%

Assessment of Blood Biophysical Properties Using Pressure Sensing with Micropump and Microfluidic Comparator

Kang

2022

Micromachines

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“…Advantages include the low cost, compared to traditional and portable imaging systems [93,[98][99][100][101][102]. In addition, smartphone platforms can provide a point of care testing [93,[103][104][105][106][107][108]. Such low cost and point of care testing are beneficial to provide testing in rural or underdeveloped areas, without expensive and sometimes bulky testing equipment.…”

Section: Smartphone-based Imagingmentioning

confidence: 99%

Microscopic Imaging Methods for Organ-on-a-Chip Platforms

Buchanan

Yoon

2022

Micromachines

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Microscopic imaging is essential and the most popular method for in situ monitoring and evaluating the outcome of various organ-on-a-chip (OOC) platforms, including the number and morphology of mammalian cells, gene expression, protein secretions, etc. This review presents an overview of how various imaging methods can be used to image organ-on-a-chip platforms, including transillumination imaging (including brightfield, phase-contrast, and holographic optofluidic imaging), fluorescence imaging (including confocal fluorescence and light-sheet fluorescence imaging), and smartphone-based imaging (including microscope attachment-based, quantitative phase, and lens-free imaging). While various microscopic imaging methods have been demonstrated for conventional microfluidic devices, a relatively small number of microscopic imaging methods have been demonstrated for OOC platforms. Some methods have rarely been used to image OOCs. Specific requirements for imaging OOCs will be discussed in comparison to the conventional microfluidic devices and future directions will be introduced in this review.

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“…The gas permeability of PDMS enables cells and various microorganisms to be cultured in the PDMS chip. The polymer is non-toxic, inexpensive and simple to manufacture, and it has become a material widely used in the manufacture of chips [ 29 , 30 , 31 , 32 ]. However, its surface is highly hydrophobic, and it is easy to adsorb some hydrophobic samples to be tested, or, bubbles are easily generated in the channel during the fabrication process [ 33 , 34 ].…”

Section: Microfluidic Chipsmentioning

confidence: 99%

Application of Microfluidic Chips in the Detection of Airborne Microorganisms

et al. 2022

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The spread of microorganisms in the air, especially pathogenic microorganisms, seriously affects people’s normal life. Therefore, the analysis and detection of airborne microorganisms is of great importance in environmental detection, disease prevention and biosafety. As an emerging technology with the advantages of integration, miniaturization and high efficiency, microfluidic chips are widely used in the detection of microorganisms in the environment, bringing development vitality to the detection of airborne microorganisms, and they have become a research highlight in the prevention and control of infectious diseases. Microfluidic chips can be used for the detection and analysis of bacteria, viruses and fungi in the air, mainly for the detection of Escherichia coli, Staphylococcus aureus, H1N1 virus, SARS-CoV-2 virus, Aspergillus niger, etc. The high sensitivity has great potential in practical detection. Here, we summarize the advances in the collection and detection of airborne microorganisms by microfluidic chips. The challenges and trends for the detection of airborne microorganisms by microfluidic chips was also discussed. These will support the role of microfluidic chips in the prevention and control of air pollution and major outbreaks.

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Smartphone supported backlight illumination and image acquisition for microfluidic-based point-of-care testing

Cited by 13 publications

References 22 publications

Assessment of Blood Biophysical Properties Using Pressure Sensing with Micropump and Microfluidic Comparator

Assessment of Blood Biophysical Properties Using Pressure Sensing with Micropump and Microfluidic Comparator

Microscopic Imaging Methods for Organ-on-a-Chip Platforms

Application of Microfluidic Chips in the Detection of Airborne Microorganisms

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