SNP detection and comparative linkage mapping of 66 bone-related genes in the pig

Onteru, Suneel Kumar; Fan, Bin; Rothschild, Max F.

doi:10.1159/000163089

Cited by 11 publications

(10 citation statements)

References 157 publications

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“…Though the mouse and rat are used as experimental models for human osteoporosis, the pig bone remodelling cycle is histologically more similar to human than the rat or mouse. Moreover, livestock genomics have many advantages over model organisms and human studies for complex trait dissection (Onteru et al, 2008). In addition to other features mentioned elsewhere in this review, the reproductive cycle of the pig is similar in duration to the human (18 to 21 days) and is continuous, also like the human (Turner, 2001).…”

Section: Bonesmentioning

confidence: 90%

Porcine models for the metabolic syndrome, digestive and bone disorders: a general overview

Litten-Brown

Corson

Clarke

2010

Animal

147

136

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The aim of this review article is to provide an overview of the role of pigs as a biomedical model for humans. The usefulness and limitations of porcine models have been discussed in terms of metabolic, cardiovascular, digestive and bone diseases in humans. Domestic pigs and minipigs are the main categories of pigs used as biomedical models. One drawback of minipigs is that they are in short supply and expensive compared with domestic pigs, which in contrast cost more to house, feed and medicate. Different porcine breeds show different responses to the induction of specific diseases. For example, ossabaw minipigs provide a better model than Yucatan for the metabolic syndrome as they exhibit obesity, insulin resistance and hypertension, all of which are absent in the Yucatan. Similar metabolic/physiological differences exist between domestic breeds (e.g. Meishan v. Pietrain). The modern commercial (e.g. Large White) domestic pig has been the preferred model for developmental programming due to the 2-to 3-fold variation in body weight among littermates providing a natural form of foetal growth retardation not observed in ancient (e.g. Meishan) domestic breeds. Pigs have been increasingly used to study chronic ischaemia, therapeutic angiogenesis, hypertrophic cardiomyopathy and abdominal aortic aneurysm as their coronary anatomy and physiology are similar to humans. Type 1 and II diabetes can be induced in swine using dietary regimes and/or administration of streptozotocin. Pigs are a good and extensively used model for specific nutritional studies as their protein and lipid metabolism is comparable with humans, although pigs are not as sensitive to protein restriction as rodents. Neonatal and weanling pigs have been used to examine the pathophysiology and prevention/treatment of microbial-associated diseases and immune system disorders. A porcine model mimicking various degrees of prematurity in infants receiving total parenteral nutrition has been established to investigate gut development, amino acid metabolism and non-alcoholic fatty liver disease. Endoscopic therapeutic methods for upper gastrointestinal tract bleeding are being developed. Bone remodelling cycle in pigs is histologically more similar to humans than that of rats or mice, and is used to examine the relationship between menopause and osteoporosis. Work has also been conducted on dental implants in pigs to consider loading; however with caution as porcine bone remodels slightly faster than human bone. We conclude that pigs are a valuable translational model to bridge the gap between classical rodent models and humans in developing new therapies to aid human health.

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Section: Bonesmentioning

confidence: 90%

Porcine models for the metabolic syndrome, digestive and bone disorders: a general overview

Litten-Brown

Corson

Clarke

2010

Animal

147

136

View full text Add to dashboard Cite

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“…Consistent with its important functions on energy balance and body weight, the FTO gene was near the QTL region for back fat and average daily gain traits in pigs (http:// www.animalgenome.org/QTLdb/pig.html) [15]. One SNP (AM931150: g.276T[G) of the FTO gene showed to be associated with intermuscular fat deposition and feed conversion rate in pigs [16].…”

Section: Introductionmentioning

confidence: 84%

Allelic Polymorphism Detected in the Bovine FTO Gene

Zhang

et al. 2011

Mol Biotechnol

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Studies in humans have independently identified single nucleotide polymorphisms (SNPs) in the fat mass and obesity associated (FTO) gene associated with obesity in multiple populations. It was shown that FTO participated in the regulation of energy homeostasis and associated with increased lipolytic activity in adipocytes. To ascertain whether there were mutations in the bovine FTO gene, this study investigated the variation of the FTO gene through PCR-SSCP and sequencing. Five synonymous mutations, two missense mutations, and three intronic SNPs were identified in 614 cattle from five independent populations. Haplotype frequencies and linkage disequilibrium (LD) coefficients of these SNPs in three Chinese indigenous cattle breeds were analyzed. Two LD blocks were found in the Qinchuan and Nanyang cattle breeds and three LD blocks were found in the Jiaxian cattle breed, suggesting the possibility of a recombination hotspot between exon 5 and intron 5 of the bovine FTO gene. The variations detected here might have an impact on the FTO gene activity and function.

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“…Genetic differences in susceptibil- 1 The online version of this article contains supplemental material. ity to develop OC are obvious from association analyses for traits related to the incidence of leg weakness and OC scores in pigs (9,44). Indeed, many loci have been associated with OC.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling of articular cartilage reveals functional pathways and networks of candidate genes for osteochondrosis in pigs

Rangkasenee

Muráni

Schellander

et al. 2013

Physiological Genomics

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SNP detection and comparative linkage mapping of 66 bone-related genes in the pig

Cited by 11 publications

References 157 publications

Porcine models for the metabolic syndrome, digestive and bone disorders: a general overview

Porcine models for the metabolic syndrome, digestive and bone disorders: a general overview

Allelic Polymorphism Detected in the Bovine FTO Gene

Gene expression profiling of articular cartilage reveals functional pathways and networks of candidate genes for osteochondrosis in pigs

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