SNPs in forensic genetics: a review on SNP typing methodologies

Sobrino, Beatriz; Brión, Marı́a; Carracedo, Ángel

doi:10.1016/j.forsciint.2004.10.020

Cited by 371 publications

(213 citation statements)

References 74 publications

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“…The majority of SNP genotyping analyses are based on: allele-specific hybridization, oligonucleotide ligation, primer extension or invasive cleavage [36]. Genotyping methods, including DNA chips, allele-specific PCR and primer extension approaches based on SNPs, are particularly attractive for their high data throughput and for their suitability for automation.…”

Section: Single Nucleotide Polymorphisms (Snps)mentioning

confidence: 99%

Assessing Plant Genetic Diversity by Molecular Tools

2009

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This paper is an overview of the diverse, predominantly molecular techniques, used in assessing plant genetic diversity. In recent years, there has been a significant increase in the application of molecular genetic methods for assessing the conservation and use of plant genetic resources. Molecular techniques have been applied in the analysis of specific genes, as well as to increase understanding of gene action, generate genetic maps and assist in the development of gene transfer technologies. Molecular techniques have also had critical roles in studies of phylogeny and species evolution, and have been applied to increase our understanding of the distribution and extent of genetic variation within and between species. These techniques are well established and their advantages as well as limitations have been realized and described in this work. Recently, a new class of advanced techniques has emerged, primarily derived from a combination of earlier, more basic techniques. Advanced marker techniques tend to amalgamate advantageous features of several basic techniques, in order to increase the sensitivity and resolution to detect genetic discontinuity and distinctiveness. Some of the advanced marker techniques utilize newer classes of DNA elements, such as retrotransposons, mitochondrial and chloroplast based microsatellites, thereby revealing genetic variation through increased genome coverage. Techniques such as RAPD and AFLP are also being applied to cDNA-based templates to study patterns of gene expression and uncover the genetic basis of biological responses. The most important and recent advances made in molecular marker techniques are discussed in this review, along with their applications, advantages and limitations applied to plant sciences.

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Section: Single Nucleotide Polymorphisms (Snps)mentioning

confidence: 99%

Assessing Plant Genetic Diversity by Molecular Tools

2009

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“…Methods using SNP markers utilize platforms/plates allowing for determination of thousands or millions of SNPs in a human population during a single DNA analysis. There are a large number of SNP methods based on hybridization, starter elongation, ligation or so-called invasive rupture, all of which favor the accessibility of this method [28].…”

Section: Single Nucleotide Polymorphism (Snp) Analysismentioning

confidence: 99%

Current methods for preimplantation genetic diagnosis

Liss¹,

Chromik²,

Szczyglińska³

et al. 2016

Ginekol Pol

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Preimplantation Genetic Diagnosis (PGD) used in assisted reproduction techniques is designed to provide help for couples trying to conceive a child, as it helps deliver healthy offspring. After in vitro fertilization, material is collected from the oocyte (polar body), 3-day-old embryo, or increasingly often, from the trophectoderm of a blastocyst. Selection of the diagnostic method depends on the testing center, but methods such as aCGH (Comparative Genomic Hybridization Array) and NGS (Next-Generation Sequencing) are supposed to have the highest reliability and precision. This paper presents a review of the most important methods used in PGD, their advantages and disadvantages as well as efficacy in the procedures in which they are used.

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“…Many studies have developed SNPs genotyping systems as reviewed by Sobrino et al 2 Although hybridization based DNA sensors are attractive for discriminating DNA specifically, they require a specific design. 3 On the other hand, an enzyme based assay can discriminate SNPs specifically based on enzyme substrate specificity and Patolsky et al reported an attractive approach based on a single base extension method.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical SNP Detection Using Glucose Dehydrogenase

et al. 2012

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Single nucleotide polymorphisms (SNPs) are important biomarkers for evaluating sensitivity to drugs and for predicting whether people might have a disease in the future. In this study, we constructed an electrochemical detection system of an SNP of peroxisome proliferator-activated receptor γ 2 (PPARγ 2 ) (C34G) using glucose dehydrogenase (GDH) based on a single base extension method. Target DNA was hybridized with capture DNA immobilized on a gold electrode. Biotinylated dCTP was inserted to probe DNA if target DNA had the SNP. Avidin conjugated GDH can bind to one base extended DNA resulting in increased response current after washing of redundant avidin conjugated GDH and addition of glucose. In this system, PPARγ 2 (C34G) was detected specifically with 10 −8 mol dm −3 detection limit based on amperometric sensing system.

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SNPs in forensic genetics: a review on SNP typing methodologies

Cited by 371 publications

References 74 publications

Assessing Plant Genetic Diversity by Molecular Tools

Assessing Plant Genetic Diversity by Molecular Tools

Current methods for preimplantation genetic diagnosis

Electrochemical SNP Detection Using Glucose Dehydrogenase

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