Social propinquity in rodents as measured by tube cooccupancy differs between inbred and outbred genotypes

Tuttle, Alexander H.; Tansley, Shannon; Dossett, Kimberly; Tohyama, Sarasa; Khoutorsky, Arkady; Maldonado‐Bouchard, Sioui; Stein, Liane; Gerstein, Lindsey; Crawhall-Duk, Hayley; Pearl, Raymond; Sukosd, Melissa; Leger, Philip; Hardt, Oliver; Yachnin, David; Austin, Jean-Sébastien; Chan, Claire M; Pooters, Tine; Groves, Isabelle; Martin, Loren J.; Sonenberg, Nahum; Gkogkas, Christos G.; Mogil, Jeffrey S.

doi:10.1073/pnas.1703477114

Cited by 18 publications

(24 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The current observations replicate the main finding of our previous study, that in the absence of pain, outbred CD‐1 mice of both sexes show higher levels of propinquity (tube co‐occupancy) if they are familiars than if they are strangers (compare sibling‐NP and stranger‐NP groups in Figure ). The main purpose of the current study was to investigate how the presence of pain might affect this sibling vs stranger difference in social behavior.…”

Section: Discussionsupporting

confidence: 90%

“…Mice in the dyad were in one of two social relation conditions: (1) sibling (born of the same parents and raised together in a single home cage from birth until weaning, and in a single home cage with same‐sex littermates from weaning until testing), or (2) stranger (born of different parents with no contact prior to testing). We previously showed that TCOT behavior is identical when mice are familiar and related compared with mice that are familiar and nonrelated, and for ethical reasons chose to test only siblings. Sibling and stranger mice were randomly assigned to one of three pain conditions: (1) no pain (NP; in which neither mouse in the dyad received any noxious stimulus), (2) one‐in‐pain (OP; in which one mouse of the dyad received the noxious stimulus but the other did not), or (3) both‐in‐pain (BP; in which both mice in the dyad received the same noxious stimulus).…”

Section: Methodsmentioning

confidence: 99%

“…Two different types of noxious stimuli were used, as described below. Although the automated scoring of tube vacancy, single occupancy, and co‐occupancy can be achieved using load cells, this system was nonfunctional at the time of data collection, which proceeded instead by video time‐sampling and manual scoring by an experimenter blinded to social relation and pain conditions. Every minute, a single 10‐second video sample was analyzed, generating percentages of samples featuring tube co‐occupancy, single occupancy, or vacancy.…”

Section: Methodsmentioning

confidence: 99%

“…Pain behavior was analyzed using repeated measures (baseline vs postinjection/surgery) ANOVA . TCOT co‐occupancy behavior in the second hour of testing was analyzed by two‐way ANOVA (social condition x pain condition) of co‐occupancy data, followed where appropriate by one‐way ANOVA s and Tukey post hoc testing. Confrontation tube test behavior (ie, percentage of “wins” per 10 trials) was analyzed by two‐way repeated measures ANOVA (dominant mouse surgical condition x subordinate mouse surgical condition) for male mice, and one‐way repeated measure ANOVA for female mice.…”

Section: Methodsmentioning

confidence: 99%

“…We recently developed a new assay of social preference based on propinquity, the willingness of two mice to co‐occupy an opaque tube placed in a stressful novel environment over a 3‐hour period, called the tube co‐occupancy test (TCOT) . In contrast to the three‐chamber test, both mice in the TCOT are unrestrained and free to make social decisions.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Modulation of social behavior and dominance status by chronic pain in mice

Tansley

Tuttle

et al. 2018

Genes Brain and Behavior

Self Cite

View full text Add to dashboard Cite

The potential influence of pain on social behavior in laboratory animals has rarely been evaluated. Using a new assay of social behavior, the tube co-occupancy test (TCOT), we assess propinquity-the tendency to maintain close physical proximity-in mice exposed to pain using subcutaneous zymosan or spared nerve injury as noxious stimuli. Our previous experience with the TCOT showed that outbred mouse sibling dyads show higher levels of tube co-occupancy than stranger dyads. We find here that long-lasting pain from spared nerve injury given to both mice in the dyad abolishes this effect of familiarity, such that strangers also display high levels of propinquity. We performed a separate experiment to assess the effect on dominance behavior of nerve injury to one or both mice of a dyad in which relative dominance status had been previously established via the confrontation tube test. We find that neuropathic pain given only to the dominant mouse reverses the relationship in male but not female mice, such that the previously subordinate mouse becomes dominant. These observations bolster the scant but growing evidence that pain can robustly affect social behavior in animals.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Modulation of social behavior and dominance status by chronic pain in mice

Tansley

Tuttle

et al. 2018

Genes Brain and Behavior

Self Cite

View full text Add to dashboard Cite

show abstract

Working with Miraculous Mice: Mus musculus as a Model Organism

Standley,

Xie,

Lau

et al. 2024

Current Protocols

View full text Add to dashboard Cite

The laboratory mouse has been described as a “miracle” model organism, providing a window by which we may gain an understanding of ourselves. Since the first recorded mouse experiment in 1664, the mouse has become the most used animal model in biomedical research. Mice are ideally suited as a model organism because of their small size, short gestation period, large litter size, and genetic similarity to humans. This article provides a broad overview of the laboratory mouse as a model organism and is intended for undergraduates and those new to working with mice. We delve into the history of the laboratory mouse and outline important terminology to accurately describe research mice. The types of laboratory mice available to researchers are reviewed, including outbred stocks, inbred strains, immunocompromised mice, and genetically engineered mice. The critical role mice have played in advancing knowledge in the areas of oncology, immunology, and pharmacology is highlighted by examining the significant contribution of mice to Nobel Prize winning research. International mouse mutagenesis programs and accurate phenotyping of mouse models are outlined. We also explain important considerations for working with mice, including animal ethics; the welfare principles of replacement, refinement, and reduction; and the choice of mouse model in experimental design. Finally, we present practical advice for maintaining a mouse colony, which involves adequate training of staff, the logistics of mouse housing, monitoring colony health, and breeding strategies. Useful resources for working with mice are also listed. The aim of this overview is to equip the reader with a broad appreciation of the enormous potential and some of the complexities of working with the laboratory mouse in a quest to improve human health. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.

show abstract

Sociability in Fruit Flies: Genetic Variation, Heritability and Plasticity

2018

View full text Add to dashboard Cite

Sociability, defined as individuals' propensity to participate in non-aggressive activities with conspecifics, is a fundamental feature of behavior in many animals including humans. However, we still have a limited knowledge of the mechanisms and evolutionary biology of sociability. To enhance our understanding, we developed a new protocol to quantify sociability in fruit flies (Drosophila melanogaster). In a series of experiments with 59 F1 hybrids derived from inbred lines, we documented, first, significant genetic variation in sociability in both males and females, with broad-sense heritabilities of 0.24 and 0.21 respectively. Second, we observed little genetic correlation in sociability between the sexes. Third, we found genetic variation in social plasticity among the hybrids, with a broad-sense heritability of ~0.24. That is, genotypes differed in the degree of sociability after experiencing the same relevant social experience. Our data pave the way for further research on the mechanisms that underlie sociability as well as its ecological and evolutionary consequences.

show abstract

Social propinquity in rodents as measured by tube cooccupancy differs between inbred and outbred genotypes

Cited by 18 publications

References 24 publications

Modulation of social behavior and dominance status by chronic pain in mice

Modulation of social behavior and dominance status by chronic pain in mice

Working with Miraculous Mice: Mus musculus as a Model Organism

Sociability in Fruit Flies: Genetic Variation, Heritability and Plasticity

Contact Info

Product

Resources

About