Sodium 1:2-naphthoquinone-4-sulfonate as a reagent for identification of amino acids and peptides and for quantitative estimation of proline and hydroxyproline separated on paper chromatograms

Qualitative Determination of Amino Acids in Protein Hydrolyzates by Circular Paper Chromatography

“…However, where the amino acids travel closely together-e.g., basic amino acids-the identification should be II. Chromatogram used to identify the following amino acids: (1) cystine, (2a) lysine, (2b) histidine, (2c) arginine, (6) alanine, (7) proline, (8a) tyrosine, (9) methionine, (10a) valine, (10b) phenylalanine, (11) leucines. Hydroxy proline and tryptophan were not present.…”

Biochemistry of the Hydroxyprolines

“…Other methods include an automated analysis of hydroxyproline (98), and application of gas chromatography (99), polarography (loo), and Folin's reagent (101). When the samples contain more than 1% hydroxyproline these methods give satisfactory results; with samples low hydroxyproline, for example, urine, the choice of a suitable procedure is important.…”

Biochemical Analysis

“…As has been true almost from the inception of paper chromatography, major interest still continues in amino acid and polypeptide separation, identification, and determination from the standpoint of method (40,169,170,172,173,177,213,296,369,391,401,403,429,512) and for numerous specific purposes, including analysis of proteins (168,281), resolution of the optical isomers of tyrosine and glutamic acid (432), determination of peptide end groups (235) and terminal amino acids of bovine plasma albumin (186), of ovomucoid (389), and of hypophysial growth hormone (300), clinical studies of amino acid metabolism (313,330), and comparative studies of the blood amino acids of mammals (129), insects (15), and silkworms (158). The specific problems of chromatography of protein split products as it applies to amino acid metabolism were reviewed by Awapara (17), and a more general comparative review of this technique in comparison with other types of fractionation procedures was published by Boulanger and Biserte (45).…”