Sodium alginate-cross-linked polymyxin B sulphate-loaded solid lipid nanoparticles: Antibiotic resistance tests and HaCat and NIH/3T3 cell viability studies

Severino, Patrícia; Chaud, Marco V.; Shimojo, Andréa Arruda Martins; Antonini, Danilo; Lancelloti, Marcelo; Santana, Maria Helena Andrade; Souto, Eliana B.

doi:10.1016/j.colsurfb.2015.03.049

Cited by 74 publications

(35 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Europe, only a few nanomedicines have yet been approved when compared to the USA [10]. The approval of nanomedicines has encountered several obstacles attributed to the difficulty of fully describing the mechanism of action and interaction of nanomedicines with the biological systems, and also of scaling-up of manufacturing and quality control methods that are important for batch to batch uniformity [11][12][13]. The European Medicines Agency (EMA) may be open to new authorizations because clinical trials with nanomedicines have being increasing in Europe in the last decades.…”

Section: Introductionmentioning

confidence: 99%

New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

Souto

Ribeiro

Ferreira

et al. 2020

IJMS

Self Cite

102

View full text Add to dashboard Cite

Burn wounds are highly debilitating injuries, with significant morbidity and mortality rates worldwide. In association with the damage of the skin integrity, the risk of infection is increased, posing an obstacle to healing and potentially leading to sepsis. Another limitation against healing is associated with antibiotic resistance mainly due to the use of systemic antibiotics for the treatment of localized infections. Nanotechnology has been successful in finding strategies to incorporate antibiotics in nanoparticles for the treatment of local wounds, thereby avoiding the systemic exposure to the drug. This review focuses on the most recent advances on the use of nanoparticles in wound dressing formulations and in tissue engineering for the treatment of burn wound infections.

show abstract

Section: Introductionmentioning

confidence: 99%

New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

Souto

Ribeiro

Ferreira

et al. 2020

IJMS

Self Cite

102

View full text Add to dashboard Cite

show abstract

“…Among the most commonly used polymeric matrices are Carbopol [89,90], alginate [91], and dextran [92]. Hao et al [93] developed a thermosensitive biohybrid hydrogel composed of poloxamer to improve the ophthalmic drug delivery.…”

Section: Innovative Hybrid Lipid Nanoparticles: Solid Lipid Nanopartimentioning

confidence: 99%

Advances in Hybrid Polymer-Based Materials for Sustained Drug Release

Ribeiro

Alcántara

Silva

et al. 2017

International Journal of Polymer Science

View full text Add to dashboard Cite

The use of biomaterials composed of organic pristine components has been successfully described in several purposes, such as tissue engineering and drug delivery. Drug delivery systems (DDS) have shown several advantages over traditional drug therapy, such as greater therapeutic efficacy, prolonged delivery profile, and reduced drug toxicity, as evidenced by in vitro and in vivo studies as well as clinical trials. Despite that, there is no perfect delivery carrier, and issues such as undesirable viscosity and physicochemical stability or inability to efficiently encapsulate hydrophilic/hydrophobic molecules still persist, limiting DDS applications. To overcome that, biohybrid systems, originating from the synergistic assembly of polymers and other organic materials such as proteins and lipids, have recently been described, yielding molecularly planned biohybrid systems that are able to optimize structures to easily interact with the targets. This work revised the biohybrid DDS clarifying their advantages, limitations, and future perspectives in an attempt to contribute to further research of innovative and safe biohybrid polymer-based system as biomaterials for the sustained release of active molecules.

show abstract

“…EE% and LC% were calculated as reported by ref. 73 using an HPLC Varian Prostar 230 (Varian, Milan, Italy) and a reversed-phase C18 column (Symmetry, 4.6 cm ×15 cm; Waters, Milan, Italy). The mobile phase consisted of methanol/CH 3 COOH (5% v/v) (60:40 v/v).…”

Section: Resultsmentioning

confidence: 99%

Synergic pro-apoptotic effects of Ferulic Acid and nanostructured lipid carrier in glioblastoma cells assessed through molecular and Delayed Luminescence studies

Grasso

Dell’Albani

Carbone

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Herein, we assessed the effect of Ferulic Acid (FA), a natural antioxidant with anti-cancer effect, on the human glioblastoma cells through molecular and Delayed Luminescence (DL) studies. DL, a phenomenon of ultra-week emission of optical photons, was used to monitor mitochondrial assessment. The effect of FA loaded in nanostructured lipid carriers (NLCs) was also assessed. To validate NLCs as a drug delivery system for glioblastoma treatment, particular attention was focused on their effect. We found that free FA induced a significant decrease in c-Myc and Bcl-2 expression levels accompanied by the apoptotic pathway activation. Blank NLCs, even if they did not induce cytotoxicity and caspase-3 cleavage, decreased Bcl-2, ERK1/2, c-Myc expression levels activating PARP-1 cleavage. The changes in DL intensity and kinetics highlighted a possible effect of nanoparticle matrix on mitochondria, through the involvement of the NADH pool and ROS production that, in turn, activates ERK1/2 pathways. All the effects on protein expression levels and on the activation of apoptotic pathway appeared more evident when the cells were exposed to FA loaded in NLCs. We demonstrated that the observed effects are due to a synergic pro-apoptotic influence exerted by FA, whose bioavailability increases in the glioblastoma cells, and NLCs formulation.Glioblastoma multiforme (GBM), also known as grade IV astrocytoma, represents the most prevalent and aggressive brain cancer. It is characterized by glial cells and has finger-like tentacles that infiltrate the brain, which make them very difficult to remove with surgical procedures. GBM exhibits a high level of resistance to conventional chemotherapy and radiotherapy, also due to the existence of blood-brain barrier (BBB), glioma stem cells and complex network of multiple modified signalling pathways 1 . The most frequent aberrant expression is represented by the dysregulation of extracellular signal-regulated protein kinase (ERK), which is associated with poor survival of the patients. The ERK isoforms (p42/44 or ERK1/ERK2) by interacting with specific phosphorylation substrates, play a pivotal role in the control of several cellular processes involved in proliferation, as well as activation of transcription factors, apoptosis and the control of cellular process 2,3 . In addition, the transcription factor c-Myc has been recognized as an important regulator of stem cell biology implicated with GBM malignancy and stemness 4 , as it contributes to proliferation, growth and survival of GBM stem cells 5 . GBM has been also related to the impairment of mitochondrial metabolic capacity, which leads to the alteration in energy production 6,7 and is characterized by an overexpression of Bcl-2 8 . This protein can regulate transition pores permeability of the outer mitochondrial membrane and block pro-apoptotic proteins 9 . Furthermore, it has been identified a novel www.nature.com/scientificreports www.nature.com/scientificreports/ interaction between Bcl-2 and (ADP-ribose) polymerase (PARP) 10 , t...

show abstract

Sodium alginate-cross-linked polymyxin B sulphate-loaded solid lipid nanoparticles: Antibiotic resistance tests and HaCat and NIH/3T3 cell viability studies

Cited by 74 publications

References 16 publications

New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

Advances in Hybrid Polymer-Based Materials for Sustained Drug Release

Synergic pro-apoptotic effects of Ferulic Acid and nanostructured lipid carrier in glioblastoma cells assessed through molecular and Delayed Luminescence studies

Contact Info

Product

Resources

About