Sodium and anion transport across the avian uterine (shell gland)epithelium

Vetter, Alisen E.; O’Grady, Scott M.

doi:10.1242/jeb.01409

Cited by 16 publications

(18 citation statements)

References 28 publications

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“…The cAMP-induced decrease in I sc is in contrast with the results obtained by Vetter and O'Grady (2005) in stripped epithelia. Those authors measured a significant higher I sc in the presence of Clafter 8-CPT-cAMP administration.…”

Section: Discussioncontrasting

confidence: 99%

“…Our results indicate an HCO 3 secretion confirmed by data obtained with the membrane-permeable cAMP analogue, 8-CPT-cAMP. The latter drug stimulated an HCO 3 secretion that was inhibited by block of carboanhydrases (Vetter and O'Grady, 2005). The pathway for this electrogenic secretion could occur via a less-selective anion conductance.…”

Section: Discussionmentioning

confidence: 98%

“…The anion should be taken up via chloride-bicarbonate exchanger and recirculated through an apical chloride channel. The latter conductance should be stimulated by cyclic adenosine monophosphate (cAMP) because an increase of shortcircuit current (I sc ) was observed in the presence of 8-(4-chlorophenylthio)-cAMP (8-CPT-cAMP), a membrane-permeable analog (Vetter and O'Grady, 2005). The present investigation was performed to clarify the controversial results and to study other possible regulatory mechanisms of anion transport.…”

Section: Introductionmentioning

confidence: 97%

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Regulation of anion transport across the uterine epithelium ofGallus domesticus

Brockmeier

Schultheiß

2011

Poultry Science

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For egg shell production, cations, primarily Ca(2+), and anions, primarily HCO(3)(-), must be secreted across the uterine epithelium. Because alterations of HCO(3)(-) transport influence Ca(2+) secretion, the present study was performed to gain insight into the regulation of anion transport (i.e., chloride and HCO(3)(-) transport) across the egg shell gland of the domestic chicken. To this purpose, unstripped uterus epithelia were mounted in modified Ussing chambers and electrogenic [i.e., short circuit current (I(sc))] and electroneutral anion transport were measured. Stimulating adenylate cyclases by forskolin, thereby enhancing the intracellular cyclic adenosine monophosphate( )(cAMP) concentration, evoked 2 patterns of I(sc) responses. Under HCO(3)(-)-buffered conditions, some of the tissues (3/8) showed an increase of I(sc), whereas in others (5/8) a decrease of I(sc) was observed in the presence of the drug. The I(sc) increase existed in HCO(3)(-) secretion because under HCO(3)(-)-free conditions forskolin stimulated only an I(sc) decrease. In addition, methazolamide, a blocker of carboanhydrases, significantly reduced baseline I(sc). The forskolin-induced decrease of I(sc) presented a Cl(-) absorption. In the absence of HCO(3)(-), forskolin yielded only an I(sc) decrease and the transepithelial flux of (36)Cl(-) was reduced. In the presence of mucosal 5-nitro-2-(3-phenylpropylamino) benzoic acid, a nonselective blocker of chloride channels, forskolin-induced I(sc) decrease was inhibited. In addition to cAMP, the regulatory influence of neurons or prostaglandins on baseline I(sc) and anion transport was investigated. Neither tetrodotoxin, a blocker of neuronal Na(+) conductances, electrical field stimulation, nor indomethacin, a blocker of cyclooxygenases, influenced the baseline I(sc) or anion transport. In summary, these data show effects of forskolin (i.e., cAMP) on anion transport across the egg shell gland of the domestic chicken: HCO(3)(-) secretion (supporting Ca(2+) mineralizing of the egg shell) and Cl(-) absorption. Neurons or prostaglandins did not influence the anion transport.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 97%

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Regulation of anion transport across the uterine epithelium ofGallus domesticus

Brockmeier

Schultheiß

2011

Poultry Science

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“…However, the plasma levels of ionized Ca 2+ do not significantly change in hens between 33 and 122 weeks of age. Thus, the decreased eggshell thickness may involve changes in shell gland function rather than availability of Ca 2+ for secretion [21]. A declining ability of the epithelium to transport Ca 2+ might be one of the major reasons for decreasing eggshell quality in older hens.…”

Section: Discussionmentioning

confidence: 99%

“…In this study we found that expression of all members of the SCNN1 gene family also increased significantly during eggshell formation in the uterus (Figure 2). The rates of Na + and anion transport are altered with age and in response to molting, which could potentially contribute to the decrease in shell thickness often associated with eggs from older birds [21]. These observations suggested a positive influence of Na + absorption on net Ca 2+ secretion [8].…”

Section: Discussionmentioning

confidence: 99%

The sodium channel gene family is specifically expressed in hen uterus and associated with eggshell quality traits

Fan

Hou

et al. 2013

BMC Genet

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BackgroundEggshell quality is important for the poultry industry. During eggshell formation a mass of inorganic minerals is deposited. The Sodium Channel (SCNN1) gene family plays an essential role in cation transportation. The objective of this study was to investigate the pattern of expression of members of the SCNN1 gene family, their variation and their effects on eggshell quality.ResultThe highest expression of SCNN1a, SCNN1b, and SCNN1g genes were in the active uterus during eggshell mineralization, while SCNN1d showed its highest expression level in the quiescent uterus (no egg present). Nineteen candidate SNPs from the four genes were genotyped in a population of 338 White Leghorn layers. Association analysis between SNPs (haplotypes/diplotypes) and eggshell traits was performed. Among seven significant SNPs, five SNPs were associated with eggshell strength, eggshell thickness, eggshell percentage or/and egg weight, while the other two SNPs within SCNN1d were only associated with eggshell percentage. These SNPs had a 0.25-6.99% contribution to phenotypic variance, depending on the trait. In haplotype analysis, SCNN1b and SCNN1d were associated with egg weight. The SCNN1b and SCNN1g were significantly associated with eggshell weight while only SCNN1g explained 2.04% of phenotypic variance. All the alleles of the members of SCNN1 gene family were associated with eggshell percentage and eggshell thickness, and others members had an association with eggshell strength except for SCNN1a. The contribution of different haplotypes of the SCNN1 gene family to eggshell phenotypic variance ranged from 0.09% to 5.74%.ConclusionsOur study indicated that the SCNN1 gene family showed tissue expression specificity and was significantly associated with eggshell traits in chicken. This study provides evidence that genetic variation in members of the sodium channel can influence eggshell quality.

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Anatomy of the female reproductive tract organs of the brown anole (Anolis sagrei)

Kircher

Stanley

Behringer

2023

The Anatomical Record

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Female reproduction in squamate reptiles (lizards and snakes) is highly diverse and mode of reproduction, clutch size, and reproductive tract morphology all vary widely across this group of ~11,000 species. Recently, CRISPR genome editing techniques that require manipulation of the female reproductive anatomy have been developed in this group, making a more complete understanding of this anatomy essential. We describe the adult female reproductive anatomy of the model reptile the brown anole (Anolis sagrei). We show that the brown anole female reproductive tract has three distinct anterior‐to‐posterior regions, the infundibulum, the glandular uterus, and the nonglandular uterus. The infundibulum has a highly ciliated epithelial lip, a region where the epithelium is inverted so that cilia are present on the inside and outside of the tube. The glandular uterus has epithelial ducts that are patent with a lumen as well as acinar structures with a lumen. The nonglandular uterus has a heterogeneous morphology from anterior to posterior, with a highly folded, ciliated epithelium transitioning to a stratified squamous epithelium. This transition is accompanied by a loss of keratin‐8 expression and together, these changes are similar to the morphological and gene expression changes that occur in the mammalian cervix. We recommend that description of the nonglandular uterus include the regional sub‐specification of a “cervix” and “vagina” as this terminology change more accurately describes the morphology. Our data extend histological studies of reproductive organ morphology in reptiles and expand our understanding of the variation in reproductive system anatomy across squamates and vertebrates.

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Sodium and anion transport across the avian uterine (shell gland)epithelium

Abstract: transport were coupled under basal and cAMP stimulated conditions and basal anion transport decreased with age and during molting.

Cited by 16 publications

References 28 publications

Regulation of anion transport across the uterine epithelium ofGallus domesticus

Regulation of anion transport across the uterine epithelium ofGallus domesticus

The sodium channel gene family is specifically expressed in hen uterus and associated with eggshell quality traits

Anatomy of the female reproductive tract organs of the brown anole (Anolis sagrei)

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