Sodium and chloride transport in tadpoles of the bullfrog Rana catesbeiana

Alvarado, RH; Moody, A

doi:10.1152/ajplegacy.1970.218.5.1510

Cited by 58 publications

(23 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While there is a multitude of studies on amphibian osmoregulatory physiology and the effects of road deicing salts on amphibian survival, we did not include species whose tolerance had been physiologically tested but never reported, even anecdotally, in saline habitats in the field (e.g., Ambystoma gracile, Alvarado and Dietz 1970a). We did include some physiological studies of species observed by others in saline habitats, even if the authors had not collected their study subjects from these habitats (e.g., Lithobates catesbeianus, Alvarado and Moody 1970). In summary, our criteria for inclusion in this review were that at least one author had found at least one life-history stage of the species alive and healthy in a saline environment, and that the account had been published.…”

Section: Methodsmentioning

confidence: 99%

Occurrence of Amphibians in Saline Habitats: A Review and Evolutionary Perspective

Hopkins

Brodie

2015

Herpetological Monographs

114

145

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Occurrence of Amphibians in Saline Habitats: A Review and Evolutionary Perspective

Hopkins

Brodie

2015

Herpetological Monographs

114

145

View full text Add to dashboard Cite

“…In this connexion it is interesting that Taylor & Barker (1965) have shown the inner face of the skin of Rana cantesbiana tadpoles to be several millivolts negative to the external solution, with the characteristic 'frog skin potential' not appearing until late metamorphosis. Alvarado & Moody (1970) found that active uptake of sodium and chloride from the bathing solution does not occur until the 'frog skin potential' appears.…”

Section: Membrane Potentials In Xenopus Embryos 327mentioning

confidence: 99%

Intracellular and intercellular potentials in the early amphibian embryo

Slack¹,

Warner²

1973

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. The sensitivity of the membrane potential of cells of early embryos of Xenopus laevis to variations in the extracellular concentrations of sodium and potassium has been determined.2. Alterations in the extracellular sodium concentration have little effect on the membrane potential at all pregastrular stages tested.3. Up to the 32 cell stage an increase in the concentration of potassium in the bathing medium causes a fall in cell membrane potential only when membrane newly synthesized in the furrow during cleavage is exposed at the embryo surface, during the second half of the cell division cycle.4. Beyond the morula stage (48 cells) a fall in membrane potential on raising external potassium can only be demonstrated when the seal which isolates the intercellular fluid from the bathing medium is broken so that cells lining the inner face of the embryo come into contact with the high potassium solution.5. The results suggest that the egg membrane has little selective permeability whereas membrane synthesized after fertilization is highly potassium permeable.6. No evidence could be obtained for any potential difference between the intercellular fluid and the external bathing medium.7. Dinitrophenol, sodium azide and cyanide prevented normal development only if they were injected into the intercellular cavity. Embryos reared in solutions containing these poisons turned into normal tadpoles.8. The formation of the intercellular cavity could be halted by injecting

show abstract

“…In the early stage of the tadpole, Na is actively transported by the gills (ALVARADO and MOODY, 1970), which begin to degenerate from stage XX (ATKINSON,1981). The active transport of Na observed in the skin appeared directly after that time.…”

Section: Discussionmentioning

confidence: 99%

Differentiation of the active sodium transport system during metamorphosis in Rana catesbeiana skin in relation to cadmium- and amiloride-induced responses.

Takada

1985

Jpn.J.Physiol

View full text Add to dashboard Cite

The differentiation of the sodium active transport system across Rana catesbeiana skin during metamorphosis was investigated and the system was analyzed by the effects of Cd2+ and amiloride. Active transport of Na in the frog skin first appeared at stage XXI of the tadpole, indicated by the appearance of a potential difference (PD) and a short circuit current (SCC) across the skin. The effects of epidermal application of Cd2+ and amiloride on the various indicators of active Na transport were as follows: (1) Cd2+ increased PD and SCC after stage XXII; (2) Cd2+ also increased the skin resistance (RM) from stage B to XXIII, but decreased it after stage XXV; and (3) amiloride decreased PD and SCC but increased RM after stage XXI. The adult frog skin can be expressed as an equivalent circuit by three parameters : ENa, the electromotive force of the active Na current, and RNa and R~, corresponding to the resistance to the active Na current and the resistance of the shunt pathway, respectively. These three parameters were calculated from the amiloride effect on PD and SCC. ENa was almost null and RNa was infinite until stage XX. ENa then gradually increased, while RNa was fairly constant between 10 and 20 kQ .cm2 after stage XXI. RE gradually increased during metamorphosis. These data suggest that there is not an active Na pathway but a passive one in the early stage of metamorphosis of the tadpole, and that the active Na transport system suddenly appears at stage XXI and gradually develops thereafter.

show abstract

Sodium and chloride transport in tadpoles of the bullfrog Rana catesbeiana

Cited by 58 publications

References 0 publications

Occurrence of Amphibians in Saline Habitats: A Review and Evolutionary Perspective

Occurrence of Amphibians in Saline Habitats: A Review and Evolutionary Perspective

Intracellular and intercellular potentials in the early amphibian embryo

Differentiation of the active sodium transport system during metamorphosis in Rana catesbeiana skin in relation to cadmium- and amiloride-induced responses.

Contact Info

Product

Resources

About