Sodium arsenate and/or urea differently affect clinical attributes, hemato-biochemistry and DNA damage in intoxicated commercial layer birds

Ghaffar, Abdul; Hussain, Riaz; Abbas, Ghulam; Ahmad, Maqsood; Abbas, Akhtar; Rahim, Yasir; Younus, Muhammad; Shahid, Muhammad; Mohiuddin, Mudassar

doi:10.1080/15569543.2017.1342096

Cited by 13 publications

(12 citation statements)

References 32 publications

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“…In present experiment, the observed lower hematological values of total red blood cell population, hematocrit and hemoglobin suggesting anemia might be cause of toxic effects of glyphosate on hematopoietic tissues. The noticeable lower red blood values have also been reported due to pesticides in cockerels and commercial layers (Hussain et al, 2017;Ghaffar et al, 2018b). The lower values of hemoglobin in cockerels appeared to be from increased oxidation of methaemoglobin or it could be due to toxic stress by the herbicide.…”

Section: Discussionmentioning

confidence: 78%

“…Previously, no reports are available about the toxic effects of glyphosate on these parameters in birds. The increased concentrations of these serum parameters in birds due to different toxic compound have been reported (Ghaffar et al, 2018b). The higher values of serum cardiac isoenzyme, cholesterol and triglycerides might be due to toxic effects of glyphosate on cardiac tissues.…”

Section: Discussionmentioning

confidence: 91%

“…For hematological and serum biochemical investigations about 5 ml blood was collected from wing vein of each cockerels on day 15, 30 and 45 of the trial and kept separately in glass test tube containing anticoagulant (EDTA; 1 mg/ml). For serum separation all the blood samples were collected without an anticoagulant and were placed on ice (Ghaffar et al, 2018b).…”

Section: Hematology and Serum Biochemistrymentioning

confidence: 99%

“…Different blood parameters (Total erythrocyte counts, hematocrit, hemoglobin concentration and total and differential leukocyte counts) were measured according to the previously described procedures (Hussain et al, 2017). Different serum biochemical parameters including aspartate aminotransferase (Cat# 12021150), alanine aminotransferase (Cat# 2022150), alkaline phosphatase (Cat# 12027150), urea (Cat# 10500400), creatinine (Cat# 389143), cardiac isoenzyme creatinine phosphokinase monobasic (Cat# 339333), cholesterol (Cat# 10150100), triglycerides (Cat # TR1697A) were measured commercial Kits with the help of chemistry analyzer (Ghaffar et al, 2018b).…”

Section: Hematology and Serum Biochemistrymentioning

confidence: 99%

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Exposure to Sub-Acute Concentrations of Glyphosate Induce Clinico-Hematological, Serum Biochemical and Genotoxic Damage in Adult Cockerels

Hussain¹

2019

PVJ

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Current experimental study was aimed to find out the clinico-hematological, serum biochemical and DNA damage impacts of commonly used herbicide (glyphosate) on non-target adult cockerels. Therefore, a total of 25 cockerels were randomly placed in wire cages in five different groups each containing five birds. After seven days of acclimatization, glyphosate-based herbicide was administered to cockerels of groups (B-E) @ 50, 75, 100 and 125 mg/kg BW respectively except group A (control) for 45 days. Blood and serum was collected at 15, 30 and 45 day of the trial from each cockerel. The treated birds at higher doses indicated different clinical signs such as ruffled feather, dullness, tremors, anemic wattle and comb, depression and reduced frequency of crowing. Feed consumption and body mass was significantly (P≤0.05) lowered in cockerels exposed to higher doses of herbicide. The hematological parameters including red blood cell counts, hematocrit and hemoglobin was significantly lower in treated cockerels. Results on different biochemical parameters showed significantly lower quantity of total proteins and albumin while significantly higher concentrations of liver function tests (alanine aminotransferase, aspartate aminotransferase, alkaline phosphate), kidney function tests (urea and creatinine), cardiac biomarkers (CK-Mb, triglycerides, cholesterol) and oxidative stress parameter (malondialdehyde) of treated cockerels in dose and time dependent manner. Results indicate that the frequency of cells with tail DNA was significantly (P≤0.001) in exposed cockerels. The findings of the study suggested that long term exposure of glyphosate induces adverse clinicohematological, serum biochemical and genotoxic effects on birds. A, 2019. Exposure to sub-acute concentrations of glyphosate induce clinicohematological, serum biochemical and genotoxic damage in adult cockerels. Pak Vet J, 39(2): 181-186. http://dx.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 91%

Section: Hematology and Serum Biochemistrymentioning

confidence: 99%

Section: Hematology and Serum Biochemistrymentioning

confidence: 99%

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Exposure to Sub-Acute Concentrations of Glyphosate Induce Clinico-Hematological, Serum Biochemical and Genotoxic Damage in Adult Cockerels

Hussain¹

2019

PVJ

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“…Biomarkers of exposure are early physiological and biochemical alterations that occur as a response of exposure to a contaminant (Hook et al, 2014;Hussain et al, 2018). Oxidative stress is one of the important biomarker of exposure and mostly used for toxicological assessments (Hameed et al, 2017;Ghaffar et al, 2018;Jaffar et al, 2019). Oxidative stress is caused by excess production of free radicles and reactive oxygen species (ROS) which readily reacts with the cellular macromolecules resulting in their impaired structures and functions (Regoli and Giuliani, 2014;Hameed et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Study of Oxidative Stress and Histo-Biochemical Biomarkers of Diethyl Phthalate Induced Toxicity in a Cultureable Fish, Labeo rohita

Latif¹,

Faheem²,

Asmatullah³

2020

PVJ

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Diethyl phthalate (DEP) is a widely used low-molecular weight phthalate which is ubiquitously detected in almost all kind of the environmental matrices. The aim of the present study was to investigate the impact of DEP-induced toxicity on a suite of biomarker responses in Labeo rohita (rohu). The median lethal concentration of DEP was found to be 4.38 mg L-1 for 96 h. Fingerlings were exposed to a sub-lethal concentration of DEP i.e. 0.51 mg L-1 (1/10 th LC50) for a period of 21 day of the experiment. Oxidative stress and histo-biochemical biomarkers were studied using gills, liver, kidney and brain tissues on 7, 14 and 21 day. The results indicated that DEP-exposure has damaged the antioxidant status by inhibiting the activities of enzymatic stress markers (catalase, glutathione-s-transferase, glutathione peroxidase, reduced glutathione) in all the studied tissues of rohu throughout the study period. Moreover, a significant increase in the levels of lipid peroxidation was observed in all the studied tissues in a time-dependent manner. The levels of hepatic-nephric biomarkers (ALT, AST, ALP, Urea and Creatinine) were found to be significantly elevated for DEP-exposed rohu when compared to control (P<0.05) throughout the study period. The histopathological marker showed severe lesions in the gills (hypertrophy, fusion and curling of lamellae,), liver (pyknotic nuclei, leukocytes infiltration and vacuolization) and kidney tissues (glomerulus expansion, narrowing and elongation of renal tubules) of DEP-exposed rohu. In conclusion, the above mentioned histo-biochemical parameters could be used as biomarkers for DEP toxicity monitoring in the aquatic ecosystem.

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Fipronil (Phenylpyrazole) induces hemato-biochemical, histological and genetic damage at low doses in common carp, Cyprinus carpio (Linnaeus, 1758)

et al. 2018

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Sodium arsenate and/or urea differently affect clinical attributes, hemato-biochemistry and DNA damage in intoxicated commercial layer birds

Cited by 13 publications

References 32 publications

Exposure to Sub-Acute Concentrations of Glyphosate Induce Clinico-Hematological, Serum Biochemical and Genotoxic Damage in Adult Cockerels

Exposure to Sub-Acute Concentrations of Glyphosate Induce Clinico-Hematological, Serum Biochemical and Genotoxic Damage in Adult Cockerels

Study of Oxidative Stress and Histo-Biochemical Biomarkers of Diethyl Phthalate Induced Toxicity in a Cultureable Fish, Labeo rohita

Fipronil (Phenylpyrazole) induces hemato-biochemical, histological and genetic damage at low doses in common carp, Cyprinus carpio (Linnaeus, 1758)

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