Sodium arsenite-induced stress-related gene expression in normal human epidermal, HaCaT, and HEL30 keratinocytes.

Trouba, Kevin J.; Geisenhoffer, Kristen; Germolec, Dori R.

doi:10.1289/ehp.02110s5761

Cited by 13 publications

(15 citation statements)

References 66 publications

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“…However, for those cosmetic constituents with mild irritancy (PEG-400, PG), the rankings were incoherent between the different cell types. A similar discrepancy was also observed in another study of sodium arsenite [18]. We think this may be related with the genetic difference including the signal transduction pathway.…”

Section: Discussionsupporting

confidence: 84%

Application of in vitro Monolayer and Three-Dimensional Culture Systems to Evaluate the Irritancy of Cosmetic Constituents

Suh¹,

Han²,

Park³

et al. 2003

Exog Dermatol

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Background: For testing skin irritancy, many cytotoxicity tests have been carried out and evaluated. It is evident that human subjects cannot be used as the initial experimental subjects. Therefore, several models, including monolayer and three-dimensional cultures, have been tested to measure skin irritancy. Objective: This study was done to compare several alternative in vitro methods used for evaluating the irritancy of cosmetic constituents. Methods: Cytotoxicity was measured by checking ED50 values with the MTT assay. The irritancy ranking of the five irritants was analyzed using the ED50 values. Results: In terms of irritancy ranking, good correlations were obtained between the monolayer and three-dimensional culture systems, although the concentrations of irritants needed to obtain the ED50 values differed considerably. Conclusion: These findings suggest that caution is necessary in the evaluation of the results of such tests, because the concentration of irritants needed to obtain the ED50 values may differ depending on the culture model used.

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Section: Discussionsupporting

confidence: 84%

Application of in vitro Monolayer and Three-Dimensional Culture Systems to Evaluate the Irritancy of Cosmetic Constituents

Suh¹,

Han²,

Park³

et al. 2003

Exog Dermatol

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“…Among the elevated genes, serine/ threonine 25 (STK25) is the oxidate stress-activated serine/ threonine kinase that may play a role in the environmental stress. For example, it was reported that STK25 can be induced by sodium arsenite, which is an environmental important carcinogen that poses a significant health risk in humans [25]. Because low oxygen tension is also the negative factor for mammalian cells, hypoxia stress is expected to induce the expression of stress responsive genes such as STK25.…”

Section: Discussionmentioning

confidence: 99%

Hypoxia induces the activation of human hepatic stellate cells LX‐2 through TGF‐β signaling pathway

et al. 2006

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Hypoxia is a common environmental stress factor and is also associated with various physiological and pathological conditions such as fibrogenesis. The activation of hepatic stellate cells (HSCs) is the key event in the liver fibrogenesis. In this study, the behavior of human HSCs LX-2 in low oxygen tension (1% O 2 ) was analyzed. Upon hypoxia, the expression of HIF-1a and VEGF gene was induced. The result of Western blotting showed that the expression of a-SMA was increased by hypoxic stimulation. Furthermore, the expression of MMP-2 and TIMP-1 genes was increased. Hypoxia also elevated the protein expression of the collagen type I in LX-2 cells. The analysis of TGF-b/ Smad signaling pathway showed that hypoxia potentiated the expression of TGF-b1 and the phosphorylation status of Smad2. Gene expression profiles of LX-2 cells induced by hypoxia were obtained by using cDNA microarray technique.

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“…Low doses of LPS (10 ng/mL) and sodium arsenite (5M), which are both oxidative stress inducers, [13][14][15] were used to treat human PBMCs. The transcriptional expression fold changes of VNN1 and PPAR␥ in treated cells compared with nontreated cells after 12 hours of treatment are shown in Figure 4.…”

Section: Expression Of Vnn1 In Human Blood Cells and The Reciprocal Ementioning

confidence: 99%

The role of vanin-1 and oxidative stress–related pathways in distinguishing acute and chronic pediatric ITP

Zhang¹,

Shen³

et al. 2011

Blood

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Sodium arsenite-induced stress-related gene expression in normal human epidermal, HaCaT, and HEL30 keratinocytes.

Cited by 13 publications

References 66 publications

Application of in vitro Monolayer and Three-Dimensional Culture Systems to Evaluate the Irritancy of Cosmetic Constituents

Application of in vitro Monolayer and Three-Dimensional Culture Systems to Evaluate the Irritancy of Cosmetic Constituents

Hypoxia induces the activation of human hepatic stellate cells LX‐2 through TGF‐β signaling pathway

The role of vanin-1 and oxidative stress–related pathways in distinguishing acute and chronic pediatric ITP

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