Sodium bisulfite enhances photosynthesis in rice by inducing Rubisco activase gene expression

Abstract

To investigate how bisulfite promotes photosynthesis, a pot experiment was conducted with rice (Oryza sativa L.) plants to determine Rubisco activity and content, and Rubisco activase (RCA) gene expression after spraying NaHSO 3 on rice leaves. The NaHSO 3 treatment promoted significantly net photosynthetic rate (P N ), carboxylation efficiency, maximum carboxylation rate, ribulose-1,5-bisphosphate regeneration rate, initial Rubisco activity, and RCA protein and mRNA concentrations. Therefore, the NaHSO 3 enhancement of P N could be directly attributed to induction of RCA gene expression both at the transcription and translation levels. Thus, the increased RCA regulated the initial Rubisco activity in vivo.

Salicylic acid-induced photosynthetic adaptability of Zea mays L. to polyethylene glycol-simulated water deficit is associated with nitric oxide signaling

“…The activation of Rubisco by RCA serves an important regulatory function in linking the rate of CO 2 fixation to the rate of electron transport activity via the production of ATP (Dias et al 2010, Parry et al 2013. Rubisco catalyzes the first step in the net photosynthetic CO 2 assimilation (Chen et al 2014b), and its activation state is regulated by RCA (Boex-Fontvieille et al 2014). In this study, water loss caused de-activation of Rubisco and RCA, which in turn caused a metabolic limitation to photosynthesis, resulting in a drop of net formation of carbohydrates, such as dry mass.…”

“…In the present study, Rbc S showed some upregulation, but Rbc L was significantly up-regulated by SA, suggesting that SA could enhance Rubisco biosynthesis under WD. Two RCA genes, ZmRCAα and ZmRCAβ, encoding larger and smaller polypeptides of approximately 46 and 43 kD were found in maize (Yin et al 2014), which ensure a stable RCA structure and maintain initial Rubisco activity under stress conditions (Wang et al 2010, Chen et al 2014b). Different RCA subunits may play different roles in photosynthetic acclimation.…”

“…It is reported that to adapt to WD stress, plants have evolved several photosynthetic mechanisms, such as, down-regulating the activity of enzymes related to the Calvin cycle, particularly Rubisco and Rubisco activase (RCA) involved in photochemistry (Dias et al 2010, Xu et al 2013). Thus, it is well known that the increase in photosynthesis can be achieved by enhancing the Rubisco and RCA activities (Carmo-Silva et al 2013, Chen et al 2014b). However, the role of SA in inducing photosynthetic ability in response to WD through activation of Rubisco and RCA is not clear.…”

Comparative proteomic analysis of autotetraploid and diploid Paulownia tomentosa reveals proteins associated with superior photosynthetic characteristics and stress adaptability in autotetraploid Paulownia

“…ATPase family, which utilizes ATP hydrolysis to remove tight-binding inhibitors and activate the catalytic activity of Rubisco (Henderson et al 2013). It was reported that photosynthesis in rice can be improved by inducing RCA gene expression (Chen et al 2014). Hence, the increased RCA may regulate the initial Rubisco activity, which will be beneficial to carbon fixation.…”

Low concentrations of glycine inhibit photorespiration and enhance the net rate of photosynthesis in Caragana korshinskii

“…HSO 3 -could block photorespiration process by forming α-hydroxylsulphonates with glyoxylate to inhibit GO activity (Zelitch 1957). Numerous researches indicated that the application of NaHSO 3 to plants was shown to increase P N in many species (Wang et al 2003, Guo et al 2006, Yang et al 2008, Chen et al 2014, Liu et al 2015. In this experiment, all NaHSO 3 treatments depressed P R .…”