Sodium dodecyl sulfate-induced rapid gelation of silk fibroin

Wu, Xi; Hou, Jing; Li, Mingzhong; Wang, Jiangnan; Kaplan, David L.; Lu, Shenzhou

doi:10.1016/j.actbio.2012.03.007

Cited by 141 publications

(151 citation statements)

References 36 publications

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“…This strategy, however, cannot be easily used for naturally derived biopolymers without chemical modification to link specific reactive groups on the polymer chains. Silk fibroin forms hydrogels when the solution was mixed with acids, salts, alcohols, surfactants and polymers [12][13][14][15]. However, toxicity of these chemicals is a significant concern for medical applications.…”

Section: Introductionmentioning

confidence: 99%

Injectable silk-polyethylene glycol hydrogels

et al. 2015

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Section: Introductionmentioning

confidence: 99%

Injectable silk-polyethylene glycol hydrogels

et al. 2015

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“…However, all experiments conrm that HA does not promote the folding of silk broin but at best it can speed up the b-sheet formation when an annealing treatment is employed. 7,10 Besides, other studies revealed how amphiphilic molecules such as surfactants can trigger a faster bsheet formation in silk broin aqueous solutions, 8,9 thus suggesting how they can be used as gelling additives. This effect has been attributed to the interaction of the surfactant with broin, through hydrogen bonding and hydrophobic interactions that accelerate protein a-helix unfolding and hydrophobic coil aggregation into a b-sheet conformation.…”

Section: Resultsmentioning

confidence: 98%

Interpenetrated 3D porous scaffolds of silk fibroin with an amino and octadecyl functionalized hyaluronic acid

et al. 2015

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An ethylenediamine (EDA) and octadecylamine (-C 18 ) hyaluronic acid (HA) derivative, named HA-EDA-C 18 , has been used for the production of interpenetrated composite biomaterials with silk fibroin. The peculiar ionic strength sensibility of this HA derivative allows the production of porous matrices without the need for chemical crosslinking. Scaffolds have been produced through a salt leaching procedure by exploiting the properties of silk fibroin and HA-EDA-C 18 to physically crosslink when forced through a syringe loaded with NaCl. The porosity of the sponges, comprised between 70-80%, was dependent on the amount of each polymer and NaCl size distribution. Moreover, through FT-IR analysis, it has been evaluated how the blend composition influences the conformational changes of fibroin. This study demonstrates that HA-EDA-C 18 induces the transition of silk fibroin to its b-sheet conformation. Moreover, the HA-EDA-C 18 weight fraction in composite sponges regulates the scaffold susceptibility to protease and hyaluronidase. FT-IR analysis and enzymatic hydrolysis studies suggest that the homogeneous interpenetration of polymers occurs. In vitro studies indicate that the presence of fibroin improves the viability and attachment of bovine chondrocytes.

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“…For each type, a minimum of six samples were measured and the OD values were averaged to determine the progression of the OD changes with time. [18,19,[23][24][25][26] The gelation of the mixed solution was non-instantaneous, requiring some time before changes in OD were observed. The time required to reach the point at which the most rapid OD changes were observed was regarded as the corresponding GT.…”

Section: Preparation Of Silk Protein Fibroin (Sf) Solutionmentioning

confidence: 98%

“…The degummed silk fibers were dissolved in a 9.3 M LiBr solution at 60°C for 1 h and dialyzed with cellulose tubular membranes (MWCO, 8000-14000) against distilled water for at least three days. [7,18,19,22] The concentration of SF was ascertained by weighing the remaining solid of a known volume of silk solution after drying at 105°C. SF solution was kept aside in a 4°C refrigerator for future use.…”

Section: Preparation Of Silk Protein Fibroin (Sf) Solutionmentioning

confidence: 99%

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Potential of biocompatible regenerated silk fibroin/sodium N-lauroyl sarcosinate hydrogels

Fang

Zhu

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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Hydrogels are becoming widely used in biomaterial applications. The available methods for the preparation of these materials are continually growing. The gelation time (GT) of silk protein fibroin is difficult to control by physical methods. The cross-linkers used in available chemical techniques are likely to impact the biocompatibility of the resultant materials. In this paper, we demonstrate that the addition of sodium N-lauroyl sarcosinate (an amino-acid-based surfactant) accelerates the formation of hydrogels from fibroin. GT, turbidity variations, changes of viscoelasticity during the gelation process, and the mechanical properties of the products are measured. The secondary structure was probed by Fourier transform infrared spectroscopy, X-ray diffraction and the morphologies of the products were investigated by scanning electron microscopy. Transformations in the β-sheet content were monitored by the fluorescence of Thioflavine T and circular dichroism measurements. The relationship between the surface tension of sodium N-lauroyl sarcosinate and the GT was also explained. To investigate cell compatibility, fibroblast cells were seeded onto the surface of the hydrogels. The results indicate that the sodium N-lauroyl sarcosinate/fibroin GT can be controlled. This blend-hydrogel demonstrates excellent cell compatibility, good compression strength, and outstanding compression-recovery characteristics. Sodium N-lauroyl sarcosinate/silk fibroin hydrogels containing β-sheets have considerable potential as replacement materials in addressing the tissue defects involved with repair surgery.

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Sodium dodecyl sulfate-induced rapid gelation of silk fibroin

Cited by 141 publications

References 36 publications

Injectable silk-polyethylene glycol hydrogels

Injectable silk-polyethylene glycol hydrogels

Interpenetrated 3D porous scaffolds of silk fibroin with an amino and octadecyl functionalized hyaluronic acid

Potential of biocompatible regenerated silk fibroin/sodium N-lauroyl sarcosinate hydrogels

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