Sodium hyaluronate/chitosan polyelectrolyte complex scaffolds for dental pulp regeneration: Synthesis and characterization

The Interplay of Dental Pulp Stem Cells and Endothelial Cells in an Injectable Peptide Hydrogel on Angiogenesis and Pulp Regeneration In Vivo

“…7 Although pulp regeneration studies have described several types of scaffolds, most of this work has focused on the odonto/osteogenic differentiation capacity of progenitor/ stem cells. 21,22 An increasing number of studies have suggested that odonto/osteogenic and angiogenic processes are interdependent through the close interaction of stem/progenitor cells and ECs or their progenitor cells. 23,24 We describe here, for the first time, the feasibility of using a peptide hydrogel scaffold to regulate the functions of DPSCs and HUVECs in angiogenesis and odontogenic differentiation in mono-and coculture systems.…”

Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications

“…2C and D for PEC-US and PEC, respectively) of scaffolds were observed, being the more homogenous and less open in the case of PEC-US than PEC scaffolds. For tissue engineering applications, the existence of pores in the polymeric structure is crucial for cell proliferation since they provide adequate conditions for cell migration as well as efficient transport of nutrients and metabolic wastes [36,37]. The quantitative determination of the pore size showed that the mean pore diameter of the scaffolds was 82 ± 7 lm and 115 ± 9 lm for PEC-US and PEC, respectively.…”

Scaffolds in Regenerative Endodontics: A Review.

“…In a 2:1 blend (HA/chitosan), cells were viable for 72 hours and no cytotoxicity was apparent. [93] The same group used chitosan/pectin scaffolds for bone regeneration with similarly positive results. [94] Chitosan/collagen scaffolds adsorbed with BMP7 were seeded with human adult dental pulp cells and stained positive for dentin matrix proteins DSPP and DMP1, whereas scaffolds without BMP7 were negative.…”

“…[86] Several studies using alginate and alginate/HA mixtures have been performed in bone and cartilage tissue engineering. [87,88] Alginate has been used in dental engineering to deliver cells and/or growth factors. The alginate hydrogel with either transforming growth factor (TGF)-b1 or acid treatment was applied to slices of human teeth with vital dentin-pulp complex tissues and maintained in culture.…”