The Interplay of Dental Pulp Stem Cells and Endothelial Cells in an Injectable Peptide Hydrogel on Angiogenesis and Pulp Regeneration <i>In Vivo</i>

Dissanayaka, Waruna Lakmal; Hargreaves, Kenneth M.; Jin, Lijian; Samaranayake, LP; Zhang, Chengfei

doi:10.1089/ten.tea.2014.0154

Cited by 171 publications

(159 citation statements)

References 41 publications

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“…One approach is to incorporate endothelial cell populations in the transplanted cell pool, which has been tested and studied especially in the field of bone regeneration (24). This concept has also been reported in pulp regeneration (25). However, ECs are difficult to harvest and it is certainly not feasible to obtain them from the same host as an autologous cell source.…”

Section: Discussionsupporting

confidence: 63%

Human and Swine Dental Pulp Stem Cells Form a Vascularlike Network after Angiogenic Differentiation in Comparison with Endothelial Cells: A Quantitative Analysis

Aksel

Huang

2017

Journal of Endodontics

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Introduction The aim was to quantify vascular network formation capacity after angiogenic induction of human and swine dental pulp stem cells (DPSCs) in comparison to endothelial cells. Methods Primary human (h) DPSCs or swine (s) DPSCs were induced in endothelial growth medium (EGM) for 7 days. The expression of endothelial marker, von Willebrand Factor (vWF) was determined by immunostaining. Induced (iDPSCs) and non-induced (n-iDPSCs) were seeded at different cell numbers onto Matrigel for vascular network formation assays and analyzed after 4, 8, 12, and 18 h in comparison to human endothelial cells (hMECs). Quantitative analysis of vascular tubule formation was performed using ImageJ. The vascular network formation was also conducted by co-culturing of n-iDPSCs and iDPSCs. Results vWF was detected by immunofluorescence in both n-iDPSCs and iDPSCs (human and swine). Time-lapse microscopic observation showed that the vascular network was formed by iDPSCs, but not n-iDPSCs. After 4 h, iDPSCs showed vascular network formation while FDPSCs started to aggregate and formed clusters. ihDPSCs displayed a similar capacity to form vascular networks in Matrigel compared to hMECs based on quantitative analysis. isDPSCs had a higher capacity compared to ihDPSCs or hMECs (p<0.05) in forming the network structures including segments, nodes and mesh. isDPSCs than ihDPSCs and hMECs (p<0.05). Co-culture experiment showed that n-ihDPSCs co-localized on the angiogenic tubules and vascular networks formed by ihDPSCs. Conclusions Our findings indicate that iDPSCs in combination of their non-induced counterparts may be utilized as a future clinical strategy for enhancing angiogenesis during the process of pulp-dentin regeneration.

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Section: Discussionsupporting

confidence: 63%

Human and Swine Dental Pulp Stem Cells Form a Vascularlike Network after Angiogenic Differentiation in Comparison with Endothelial Cells: A Quantitative Analysis

Aksel

Huang

2017

Journal of Endodontics

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“…[ 32,33 ] However, in vivo implantation of hDPSCs using an injectable in vivo-forming hydrogel has been little demonstrated. [34][35][36][37] Recently, we have reported an injectable in vivoforming PC hydrogel for both in vitro and in vivo applications resulted in noninvasive surgeries. [21][22][23][24][25] In addition, both in vitro-and in vivo-forming PC hydrogel maintained their structure for at least ten months with little degradation.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Osteogenic Differentiation of Human Dental Pulp Stem Cells Embedded in an Injectable In Vivo‐Forming Hydrogel

Jang

Park

et al. 2016

Macromolecular Bioscience

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In this study, human dental pulp stem cells (hDPSCs) are examined as a cellular source for bone tissue engineering using an in vivo-forming hydrogel. The hDPSCs are easily harvested in large quantities from extracted teeth. The stemness of harvested hDPSCs indicates their relative tolerance to ex vivo manipulation in culture. The in vitro osteogenic differentiation of hDPSCs is characterized using Alizarin Red S (ARS), von Kossa (VK), and alkaline phosphatase (ALP) staining. The solution of hDPSCs and a methoxy polyethylene glycol-polycaprolactone block copolymer (PC) is easily prepared by simple mixing at room temperature and in no more than 10 s it forms in vivo hydrogels after subcutaneous injection into rats. In vivo osteogenic differentiation of hDPSCs in the in vivo-forming hydrogel is confirmed by micro-computed tomography (CT), histological staining, and gene expression. Micro-CT analysis shows evidence of significant tissue-engineered bone formation in hDPSCs-loaded hydrogel in the presence of osteogenic factors. Differentiated osteoblasts in in vivo-forming hydrogel are identified by ARS and VK staining and are found to exhibit characteristic expression of genes like osteonectin, osteopontin, and osteocalcin. In conclusion, hDPSCs embedded in an in vivo-forming hydrogel may provide benefits as a noninvasive formulation for bone tissue engineering applications.

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“…DPSCs alone or primarily in coculture with Human Umbilical Vein Endothelial Cells (HUVEC) when encapsulated in three-dimensional peptide hydrogel matrices (PuraMatrix) were able to support cell survival, migration, and capillary network formation and to regenerate vascularized pulp-like tissue after transplantation in mice [126]. Iohara et al [127] were able to isolate and characterize a highly vasculogenic subfraction of side population (SP) of CD31−/CD146− porcine tooth germ-derived dental MSCs, while in later study the CD31− pulp fraction was used successfully to reconstitute blood flow and capillary density in a mouse hindlimb ischemia model, to induce neurogenesis in a cerebral ischemia model, and finally to reinstitute a vascularized pulp in an ectopic root transplantation model [116].…”

Section: Differentiation Potential and Paracrine Activity Of Dentamentioning

confidence: 99%

Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application

Bakopoulou

About

2016

Stem Cells International

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Dental Mesenchymal Stem Cells (MSCs), including Dental Pulp Stem Cells (DPSCs), Stem Cells from Human Exfoliated Deciduous teeth (SHED), and Stem Cells From Apical Papilla (SCAP), have been extensively studied using highly sophisticated in vitro and in vivo systems, yielding substantially improved understanding of their intriguing biological properties. Their capacity to reconstitute various dental and nondental tissues and the inherent angiogenic, neurogenic, and immunomodulatory properties of their secretome have been a subject of meticulous and costly research by various groups over the past decade. Key milestone achievements have exemplified their clinical utility in Regenerative Dentistry, as surrogate therapeutic modules for conventional biomaterial-based approaches, offering regeneration of damaged oral tissues instead of simply “filling the gaps.” Thus, the essential next step to validate these immense advances is the implementation of well-designed clinical trials paving the way for exploiting these fascinating research achievements for patient well-being: the ultimate aim of this ground breaking technology. This review paper presents a concise overview of the major biological properties of the human dental MSCs, critical for the translational pathway “from bench to clinic.”

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The Interplay of Dental Pulp Stem Cells and Endothelial Cells in an Injectable Peptide Hydrogel on Angiogenesis and Pulp Regeneration In Vivo

Cited by 171 publications

References 41 publications

Human and Swine Dental Pulp Stem Cells Form a Vascularlike Network after Angiogenic Differentiation in Comparison with Endothelial Cells: A Quantitative Analysis

Human and Swine Dental Pulp Stem Cells Form a Vascularlike Network after Angiogenic Differentiation in Comparison with Endothelial Cells: A Quantitative Analysis

In Vivo Osteogenic Differentiation of Human Dental Pulp Stem Cells Embedded in an Injectable In Vivo‐Forming Hydrogel

Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application

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