Sodium Nitroprusside and Putrescine Mitigate PEG-Induced Drought Stress in Seedlings of Solanum lycopersicum

Abstract

Crop plants all over the world face an array of environmental threats among which drought is found to be the most deleterious. The present investigation was carried to test the efficacy of exogenously supplemented sodium nitroprusside (SNP), a nitric oxide donor, and polyamine (PA) putrescine (PUT) in mitigating the effects of polyethylene glycol (PEG)-imposed drought stress in in vitro grown seedlings of Solanum lycopersicum. PEG treatments at different concentrations (1, 2, 5, 10, and 15%) were assessed for their impact on plant growth parameters based on which 10% PEG was chosen as effective drought treatment. Varying concentrations of SNP (100, 150, and 200 µM) and PUT (0.1, 0.3, and 0.5 mM) at different combinations were studied for their mitigating effects. The results revealed that among the combinations tested, SNP (150 µM) + PUT (0.3 mM) resulted in effective mitigation of drought stress in S. lycopersicum seedlings. SNP and PUT supplementation enhanced the growth parameters such as shoot and root length and plant fresh weight (FW). Further, the relative water content (RWC), chlorophyll, and proline contents were significantly enhanced in the experimental plants as compared to the control. Effective reduction in the malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ), a significant increase in the antioxidant enzymes, and the transcriptional level upregulation of antioxidant genes and drought-responsive genes were observed in the treated plants as compared to the control. These results suggest that exogenous supplementation of SNP and PUT has a positive influence on alleviating the drought stress in S. lycopersicum.

Effects of Exogenous Sodium Nitroprusside Spraying on Physiological Characteristics of Soybean Leaves at the Flowering Stage under Drought Stress

“…Hogg et al [42] showed that NO effectively reduces membrane lipid peroxidation. When Sundararajan et al [43] carried out drought stress on seedlings of Solanum lycopersicum, it was found that the intervention of NO reduced the content of MDA in plants and improved the ability of plants to cope with drought stress, which was consistent with the results of this experiment. To sum up, spraying SNP treatment can reduce the MDA content in the soybean body and reduce the peroxidation degree of soybean membrane lipid, and the drought-resistant soybean varieties with different degrees of drought sensitivity can reduce the MDA content more.…”

Effects of Irrigation with Sodium Nitroprusside on Physiological Characteristics of Soybean under Drought Stress

“…U nder different concentrations of SNP treatment, the MDA content of the two soybean leaves decreased to varying degrees. This study's findings are in line with a study by Sundararajan et al (2022) that demonstrated sodium nitroprusside's ability to lower MDA levels in tomato seedlings and increase drought tolerance in tomatoes. W hen soybeans were treated with varying concentrations of SNP, the MDA level of the two soybean leaves first decreased and subsequently increased as treatment duration increased.…”

Physiological and Biochemical Effects of Drought Stress in Stevia (Stevia rebaudiana Bertoni)

“…Kuraklık stresi altında H2O2 miktarı, kontrol bitkileri ile kıyaslandığında, STF-4 çeşidinde %5,5 artarken Yalova çeşidinde %42,7 azalmıştır. Literatürde, kuraklık stresinin Phaseolus vulgaris [52], Ginkgo biloba [53], Vigna mungo [54] ve Solanum lycopersicum [55] bitkilerinde H2O2 miktarında artışa neden olduğu rapor edilmiştir. STF-4 çeşidinde kuraklık stresi altında H2O2 miktarındaki artış, membran kararlılığının korunduğunu ve stresin meydan getirdiği hasarı tolere edebildiğini göstermektedir.…”