Sodium sulphite yields improved DNA of higher stability for PCR detection of Citrus yellow mosaic virus from citrus leaves

Baranwal, V. K.; Majumder, S.; Ahlawat, Y. S.; Singh, R. P.

doi:10.1016/s0166-0934(03)00188-5

Cited by 38 publications

(21 citation statements)

References 14 publications

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“…The quantity of the DNA obtained from six samples was more CTAB method followed by sodium sulphite method. Similar results were obtained by Baranwal et al (2003) where commercial kit and CTAB method were used while working with potato and a cherry virus and with citrus yellow mosaic virus. However, the yield of DNA by com- mercial kit (159.60 ng/ml) was much less as against sodium sulphate method (1216.16 ng/ml).…”

Section: Determination Of Quality Of Dna Isolated By Three Methodssupporting

confidence: 78%

Rapid molecular detection of Candidatus Liberibacter asiaticus, the bacterium associated with citrus huanglongbing (greening) disease of Mandarin using polymerase chain reaction

Mahajan¹,

Jagtap²,

Dey³

2013

Afr. J. Biotechnol.

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Huanglongbing (HLB or citrus greening or yellow shoot disease) is a devastating disease of citrus caused by nonculturable, fastidious phloem limited bacterium, Candidatus Liberibacter asiaticus and threatens the citrus industry in Asian countries. The putative causal agent of the disease is transmitted through insect vector or grafting with diseased budwood. The polymerase chain reaction (PCR) diagnosis is a more reliable and sensitive diagnostic tool for detecting greening bacterium than other conventional approaches like electron microscopy, DNA-DNA hybridization and immunofluorescence (IF) for detection of citrus greening. Results reveal that sodium sulphite method of DNA isolation provided higher yield and better quality DNA than other methods. To confirm the reliability of PCR, the greening bacterium was also detected in graft-inoculated plants, which showed typical greening symptoms. Results show amplification of 450 bp in PCR suggesting sampling in March is more suitable for PCR detection of greening bacterium. The methods validated in this study will be very useful for regulatory response, effective management of infected trees, and development of a Candidatus Liberibacter asiaticus free nursery system.

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Section: Determination Of Quality Of Dna Isolated By Three Methodssupporting

confidence: 78%

Rapid molecular detection of Candidatus Liberibacter asiaticus, the bacterium associated with citrus huanglongbing (greening) disease of Mandarin using polymerase chain reaction

Mahajan¹,

Jagtap²,

Dey³

2013

Afr. J. Biotechnol.

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“…In order to prevent interference of the polyphenolics in nucleic acid extraction the addition of sodium sulfi te to the extraction buffer has reduced the degradation of DNA and improved the yields, quality and stability of DNA (Byrne et al, 2001). Sodium sulfi te-extracted DNA survived at various temperatures much longer than that extracted without addition of sodium sulfi te (Baranwal et al, 2003). Jagoueix et al (1996), Nakashima et al (1996) and Krishna Reddy (2000) Brought to you by | MIT Libraries Authenticated Download Date | 5/9/18 9:10 PM PCR method for amplifying the 16S rDNA fragment of the HLB pathogen by using HLB-specifi c primers.…”

Section: Resultsmentioning

confidence: 99%

Detection of Huanglongbing (Citrus Greening) Disease by Nucleic Acid Spot Hybridization

Gopal

Sudarsan²,

Gopi

et al. 2009

Zeitschrift Für Naturforschung C

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Polymerase chain reaction (PCR) amplifi cation with primers specifi c to the rDNA region successfully amplifi ed the 1160-bp DNA fragment from a Huanglongbing (HLB)-infected sweet orange sample with mottling symptoms leaves, but not from healthy sweet orange plants. The PCR product of 1160-bp was used as probe labeled with biotin for detection of the HLB pathogen in the nucleic acid spot hybridization (NASH) test. It was found that the HLB pathogen could be detected up to 1:100 dilution in HLB-infected tissue. Total DNA extracted from HLB-infected tissue was diluted 2-fold as 900 ng in TE buffer and spotted on a nitrocellulose membrane. Strong signals were observed up to 225 ng of DNA dilution, whereas a moderate signal was recorded at 112 ng. No hybridization signal was observed in the healthy samples, while strong signals were observed in the positive control.

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“…Symptomless nature of a few badnaviruses such as Banana streak virus in banana (Lockhart 1995), Citrus yellow mosaic virus (CYMV) in citrus (Baranwal et al 2003) and Sugarcane mosaic virus in sugarcane (Lockhart and Autrey 1988) are reported. However, so far there is no experimental evidence as to …”

Section: Archives Of Phytopathology and Plant Protection 1003mentioning

confidence: 99%

Occurrence of symptomless source ofPiper yellow mottle virusin black pepper (Piper nigrumL.) varieties and a wild Piper species

Bhat

Siljo

Devasahayam

2012

Archives Of Phytopathology And Plant Protection

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Symptomless nature of Piper yellow mottle virus (PYMoV) infection in three varieties of black pepper (Piper nigrum) (Panniyur 1, Panniyur 5 and Panchami) and a wild species of Piper (Piper colubrinum) was confirmed by polymerase chain reaction (PCR) using PYMoV specific primers. The virus could be transmitted from these PYMoV-infected symptomless plants onto symptom producing black pepper cv. Karimunda through mealybug vector, Ferrisia virgata and by graft transmission. About 20-50% seedlings showed typical symptoms of the PYMoV at 30 days after mealybug inoculations while it was 75-94% at 90 days after inoculation. PCR test of the inoculated seedlings confirmed the presence of PYMoV in 50-64%, 76-100% and 80-100% of plants in 30, 60 and 90 days after inoculation, respectively. Similarly, 50-66%, 91-100% and 100% of grafttransmitted plants showed typical symptoms of the disease at 30, 60 and 90 days after grafting. PCR test of the graft-transmitted plants showed 100% PYMoV infection at 60 days after grafting. The results clearly demonstrated the existence of PYMoV-infected symptomless plants that can act as source for secondary spread of the virus in the field.

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Sodium sulphite yields improved DNA of higher stability for PCR detection of Citrus yellow mosaic virus from citrus leaves

Cited by 38 publications

References 14 publications

Rapid molecular detection of Candidatus Liberibacter asiaticus, the bacterium associated with citrus huanglongbing (greening) disease of Mandarin using polymerase chain reaction

Rapid molecular detection of Candidatus Liberibacter asiaticus, the bacterium associated with citrus huanglongbing (greening) disease of Mandarin using polymerase chain reaction

Detection of Huanglongbing (Citrus Greening) Disease by Nucleic Acid Spot Hybridization

Occurrence of symptomless source ofPiper yellow mottle virusin black pepper (Piper nigrumL.) varieties and a wild Piper species

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