2020
DOI: 10.1364/optica.399600
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SOFISM: Super-resolution optical fluctuation image scanning microscopy

Abstract: Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging sub-wavelength-sized objects, leaving its mark in multiple branches of biology and technology. While several super-resolution optical microscopy methods have become a common tool in life science imaging, new methods, supported by cutting-edge technology, continue to emerge. One rather recent addition to the super-resolution toolbox, image scanning microscopy (ISM), achieves up to twofold lateral resolution enhanceme… Show more

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Cited by 46 publications
(35 citation statements)
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“…This fluctuation time scale is far below the minimal exposure time for the cameras discussed in the previous section (roughly 1 ms). These results, together with recent work on SOFI in scanning mode [57] (SOFISM, see section 3.1.3), demonstrate the great potential of SOFI microscopy performed with high-temporal resolution SPAD arrays.…”
Section: Upcoming Technologies-spad Arrays For Imaging Fluctuationssupporting
confidence: 62%
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“…This fluctuation time scale is far below the minimal exposure time for the cameras discussed in the previous section (roughly 1 ms). These results, together with recent work on SOFI in scanning mode [57] (SOFISM, see section 3.1.3), demonstrate the great potential of SOFI microscopy performed with high-temporal resolution SPAD arrays.…”
Section: Upcoming Technologies-spad Arrays For Imaging Fluctuationssupporting
confidence: 62%
“…Conceptually, the combination of SOFI analysis with a CLSM system provides a robust super-resolution imaging method that maintains the capacity of CLSM to perform 3D imaging [57]. However, due to the point-by-point scanning mode, the acquisition time of such a combination is substantially longer than that of conventional SOFI.…”
Section: Sofi In Scanning Modementioning
confidence: 99%
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“…Furthermore, when the detector array also provides a high temporal resolution (more than one megahertz), the combined spatiotemporal information can be used to augment the information content provided by a single FFS experiment ( 15 , 16 ), i.e., the so-called comprehensive correlation analysis. The same spatiotemporal information combined with a similar correlation analysis was used to implement nanoscopy imaging ( 17 ). Finally, by combining the spatial and timing information, super-resolution fluorescence lifetime imaging ( 14 ) and nanoscopy techniques based on photon coincidences (quantum microscopy) become feasible ( 18 ).…”
Section: Introductionmentioning
confidence: 99%
“…In the process of imaging, the useful information can be contained not only in the light field but also in the light intensity fluctuations [1,2,3]. The role of light intensity fluctuations in improving the resolution of optical image has been proved [4,5,6,7]. The impact of light intensity fluctuations on the visibility of ghost imaging, ghost interference, and ghost diffraction were reported in [8,9,10,11,12].…”
Section: Introductionmentioning
confidence: 99%