Soft lithography detects partial mechano-sensoric blindness to micrometre topography in cultured aged and diseased cells

Kaufmann, Dieter; Su, Yi; Biela, Sarah; Jungbauer, Simon; Spatz, Joachim P.; Kemkemer, Ralf

doi:10.3139/146.110533

Cited by 3 publications

(2 citation statements)

References 40 publications

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“…Since many details about cellular mechanosensoric responses to geometry and topography are not understood, we can only speculate about the underlying reasons for this altered behavior of the NF1 , 2010]. This hypothesis is supported by the observation of actin stress fiber alignment along the grooves [Teixeira et al, 2003;Gerecht et al, 2007;Kaufmann et al, 2011]. Moreover, experiments by others demonstrated that actin filament dynamics is affected by a reduced neurofibromin dose in human fibrosarcoma cell line via changes in the Rho-ROCK-LIMK2-Cofilin pathway [Ozawa et al, 2005].…”

Section: Discussionmentioning

confidence: 57%

Partial Blindness to Submicron Topography in NF1 Haploinsufficient Cultured Fibroblasts Indicates a New Function of Neurofibromin in Regulation of Mechanosensoric

Kaufmann

Hoesch

Su³

et al. 2012

Mol Syndromol

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Cells sense physical properties of their extracellular environment and translate them into biochemical signals. In this study, cell responses to surfaces with submicron topographies were investigated in cultured human NF1 haploinsufficient fibroblasts. Age-matched fibroblasts from 8 patients with neurofibromatosis type 1 (NF1^+/–) and 9 controls (NF1^+/+) were cultured on surfaces with grooves of 200 nm height and lateral distance of 2 µm. As cellular response indicator, the mean cell orientation along microstructured grooves was systematically examined. The tested NF1 haploinsufficient fibroblasts were significantly less affected by the topography than those from healthy donors. Incubation of the NF1^+/– fibroblasts with the farnesyltransferase inhibitor FTI-277 and other inhibitors of the neurofibromin pathway ameliorates significantly the cell orientation. These data indicate that NF1 haploinsufficiency results in an altered response to specific surface topography in fibroblasts. We suggest a new function of neurofibromin in the sensoric mechanism to topographies and a partial mechanosensoric blindness by NF1 haploinsufficiency.

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Section: Discussionmentioning

confidence: 57%

Partial Blindness to Submicron Topography in NF1 Haploinsufficient Cultured Fibroblasts Indicates a New Function of Neurofibromin in Regulation of Mechanosensoric

Kaufmann

Hoesch

Su³

et al. 2012

Mol Syndromol

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“…Only few studies, show age-dependent differences in the cellular response to different physical stimuli. Kaufmann et al investigated how human fibroblasts, derived from skin samples of different old donors, respond to micro-sized grooves on poly(dimethylsiloxane) (PDMS) [5]. They found that cells from old donors adapted their morphology to a less degree than cells from young donors.…”

Section: Introductionmentioning

confidence: 99%

Track D

Finocchiaro

Engwicht

Dittrich

et al. 2014

Biomedical Engineering / Biomedizinische Technik

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Increasing number of studies are focused on how adherent cells respond, in vitro, to different properties of a material. Typical properties are the surface chemistry, topographical cues (at the nano-and micro-scale) of the surface, and the substrate stiffness. Cell response studies are of importance for designing new biomaterials with applications in cell culture technologies, regenerative medicine, or for medical implants. However, only very few studies take the cell age factor, respectively the donor age, into account. In this work, we tested two types of human vascular cells (smooth muscle and endothelial cells) from old and young donors on (a) micro-structured surfaces made of poly(dimethylsiloxane) or on (b) flat polyacrylamide hydrogels with varying stiffnesses. These experiments reveal age-dependent and cell typedependent differences in the cell response to the topography and stiffness, and may establish the basis for further studies focusing on cell age-dependent responses.

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Age-dependent migratory behavior of human endothelial cells revealed by substrate microtopography

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Picart

Kemkemer

2019

Experimental Cell Research

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Soft lithography detects partial mechano-sensoric blindness to micrometre topography in cultured aged and diseased cells

Cited by 3 publications

References 40 publications

Partial Blindness to Submicron Topography in <b><i>NF1</i></b> Haploinsufficient Cultured Fibroblasts Indicates a New Function of Neurofibromin in Regulation of Mechanosensoric

Partial Blindness to Submicron Topography in <b><i>NF1</i></b> Haploinsufficient Cultured Fibroblasts Indicates a New Function of Neurofibromin in Regulation of Mechanosensoric

Track D

Age-dependent migratory behavior of human endothelial cells revealed by substrate microtopography

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