Soft scrubbing off of zona pellucida on disposable microfluidic chip

Yamanishi, Yoko; Mizunuma, Takehito; Inomata, Naoki; Kudo, Shogo; Arai, F

doi:10.1109/memsys.2010.5442516

Cited by 5 publications

(1 citation statement)

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“…The MMT driven by a permanent magnet can be applied to wide range of cell manipulations such as loader, sorter, droplet generation, etc. [9][10][11].Enucleation by MMT was conducted previously; MMT driven by the HPD with ultrasonic vibration applied to the microchip could obtain 1.1 μm in position accuracy, and also by fabricated riblet type of MMT, which could achieve high performance in speed [12][13][14]. However it is difficult to handle the oocyte to control its posture and remove only the nucleus part since the oocyte is operated in an unconfined space.…”

Section: Introductionmentioning

confidence: 99%

Continuous enucleation of bovine oocyte by microrobot with local flow distribution control

Feng

Ichikawa

Arai

et al. 2012

2012 International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3m-Nano)

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We present a continuous enucleation process of bovine oocytes on a microfluidic chip to achieve the continuous cutting of the oocytes and increasing the potential viability of the enucleated oocyte. By combining microfluidic chip and micororobotics, the flow in a channel can be actively controlled and we achieved successive operations of 1) loading oocyte, 2) control cutting volume and 3) removing nucleus. The magnetically actuated microrobot can control local fluid flow by changing its position like gate valve and control fluid force distributions in a microchip which govern oocyte movement in a chip. The optimally designed microchannel for enucleation enables continuous operation and cutting bovine oocyte with smooth manner by hydrodynamic force. The new system is propose here to show advantages by the means of the continuous operation on oocytes in a short term, cutting precision and great potentiality on continuous enucleation process for clone technology.

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