Soft Tissue Engineering

Raia, Nicole; McGill, Meghan; Marcet, Tyler; Yucha, Sarah E. Vidal; Kaplan, David L.

doi:10.1016/b978-0-12-816137-1.00087-8

Cited by 6 publications

(10 citation statements)

References 136 publications

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“…Soft tissues or specialized tissues exhibit high flexibility and a very low mechanical stiffness with a maximum of 1 MPa [ 5 ], such physiological features endow the topographical, mechanical and biochemical properties of a tissue [ 62 ]. As mentioned earlier the primary goal of this study is to design a promising model for soft tissue engineering which matches the native tissue.…”

Section: Discussionmentioning

confidence: 99%

“…The fibre reinforced composite ECM of soft tissues is mainly composed of collagen, elastin and ground substance (gelatinous amorphous material). Various probing techniques have revealed that the mechanical strength of soft tissues ranges from 0.1 kPa to 1 MPa [ 5 ]. The heterotypic fibrils of Collagen I and Collagen III present in the microenvironment of soft tissues were found to affect the mechanical properties by regulating fibril dimensions [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Adipose tissue derived stromal cells in a gelatin-based 3D matrix with exclusive ascorbic acid signalling emerged as a novel neural tissue engineering construct: an innovative prototype for soft tissue

Martin

Radhakrishnan²,

Ribelles

et al. 2022

Regenerative Biomaterials

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The current study investigated a triad, which comprises of adipose tissue derived stem cells isolated from infrapatellar fat pad and gelatin/polyvinyl alcohol (PVA)-based matrix with exclusive ascorbic acid signalling. Though, the bio-mechanical properties of the gelatin–PVA blended scaffolds in wet condition are equivalent to the ECM of soft tissues in general, in this study, the triad was tested as a model for neural tissue engineering. Apart from being cytocompatible and biocompatible, the porosity of the scaffold has been designed in such a manner that it facilitates the cell signalling and enables the exchange of nutrients and gases. The highly proliferative stem cells from Passage 2 were characterized using both, mesenchymal and embryonic stem cell markers. As an initial exploration the mesenchymal stem cells at Passage 4 were exposed to ascorbic acid and basic fibroblast growth factor signalling for neuronal differentiation in 2D environment independently. The MSCs successfully differentiated and acquired neuron specific markers related to cytoskeleton and synapses. Subsequently, three phases of experiments have been conducted on the 3D gelatin/PVA matrix to prove their efficacy, the growth of stem cells, growth of differentiated neurons and the in situ growth and differentiation of MSCs. The scaffold was conducive and directed MSCs to neuronal lineage under specific signalling. Overall, this organotypic model triad could open a new avenue in the field of soft tissue engineering as a simple and effective tissue construct.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Adipose tissue derived stromal cells in a gelatin-based 3D matrix with exclusive ascorbic acid signalling emerged as a novel neural tissue engineering construct: an innovative prototype for soft tissue

Martin

Radhakrishnan²,

Ribelles

et al. 2022

Regenerative Biomaterials

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show abstract

“…, hyaluronic acid, chondroitin sulfate), and proteogly-cans ( i.e. , aggrecan, versican), which contribute to the elasticity of these tissues[ 24 ]. Regeneration of the skin, orbit, cardiac, nerves, lung, and many other examples of soft tissues requires the introduction of several biomaterials that meet the required criteria, especially in immunocompromised models and critical-sized defects[ 23 ].…”

Section: Bioceramics In Soft Tissue Regenerationmentioning

confidence: 99%

“…Bioactive glass repaired induced ulcerative colitis wounds in rats through significant upregulation of some inflammatory pathways[ 34 ]. Despite the wide range of applications of bioceramics in soft tissue regeneration, the introduction of bioceramics in lung and cardiac tissue regeneration research is still uncommon[ 24 ].…”

Section: Bioceramics In Soft Tissue Regenerationmentioning

confidence: 99%

Advances in clinical applications of bioceramics in the new regenerative medicine era

Elshazly,

Nasr,

Hamdy

et al. 2024

World J Clin Cases

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In this editorial, we comment on the hard and soft tissue applications of different ceramic-based scaffolds prepared by different mechanisms such as 3D printing, sol-gel, and electrospinning. The new concept of regenerative medicine relies on biomaterials that can trigger in situ tissue regeneration and stem cell recruitment at the defect site. A large percentage of these biomaterials is ceramic-based as they provide the essential requirements of biomaterial principles such as tailored multisize porosity, antibacterial properties, and angiogenic properties. All these previously mentioned properties put bioceramics on top of the hierarchy of biomaterials utilized to stimulate tissue regeneration in soft and hard tissue wounds. Multiple clinical applications registered the use of these materials in triggering soft tissue regeneration in healthy and diabetic patients such as bioactive glass nanofibers. The results were promising and opened new frontiers for utilizing these materials on a larger scale. The same results were mentioned when using different forms and formulas of bioceramics in hard defect regeneration. Some bioceramics were used in combination with other polymers and biological scaffolds to improve their regenerative and mechanical properties. All this progress will enable a larger scale of patients to receive such services with ease and decrease the financial burden on the government.

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“…This means, that hydrogels made of recombinant spider silk proteins, without additional modifications, are well suited for but also limited to the biofabrication of soft tissues. Mechanical properties can, however, be increased to reach the range of muscle, skin, and cartilage tissue, [27] using chemical crosslinking and increasing the protein concentration, which is feasible up to 7% (w/v) in aqueous solution, to strengthen the hydrogel to elastic moduli up to 110 kPa. [18] The presence of salts, by adding cell culture media to the protein solutions, resulted in faster gelation and stiffer hydrogels.…”

Section: Introductionmentioning

confidence: 99%

Impact of Cell Loading of Recombinant Spider Silk Based Bioinks on Gelation and Printability

Lechner

Trossmann

Scheibel

2021

Macromolecular Bioscience

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Printability of bioinks encompasses considerations concerning rheology and extrudability, characterization of filament formation, shape fidelity, cell viability, and post-printing cellular development. Recombinant spider silk based hydrogels might be a suitable material to be used in bioinks, that is, a formulation of cells and materials to be used for bioprinting. Here, the high shape fidelity of spider silk ink is shown by bioprinting the shape and size of a human aortic valve. Further the influence of the encapsulation of cells has been evaluated on spider silk hydrogel formation, hydrogel mechanics, and shape fidelity upon extrusion based bioprinting. It is shown that the presence of cells impacts the gelation of spider silk proteins differently, depending on the used silk variant. RGD-modified spider silk hydrogels are physically crosslinked by the cells, while there is no active interaction between cells and un-tagged spider silk proteins. Strikingly, even at cell densities up to ten million cells per milliliter, cell viability is high after extrusion-based printing, which is a significant prerequisite for future applications. Shape fidelity of the printed constructs is demonstrated using a filament collapse test in the absence and presence of human cells.

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Soft Tissue Engineering

Cited by 6 publications

References 136 publications

Adipose tissue derived stromal cells in a gelatin-based 3D matrix with exclusive ascorbic acid signalling emerged as a novel neural tissue engineering construct: an innovative prototype for soft tissue

Adipose tissue derived stromal cells in a gelatin-based 3D matrix with exclusive ascorbic acid signalling emerged as a novel neural tissue engineering construct: an innovative prototype for soft tissue

Advances in clinical applications of bioceramics in the new regenerative medicine era

Impact of Cell Loading of Recombinant Spider Silk Based Bioinks on Gelation and Printability

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