Soft-tissue Wound Healing by Anti-advanced Glycation End-products Agents

Abstract

The blocking of advanced glycation end-products (AGE) has been shown to reduce diabetic complications and control periodontitis. This study investigated the pattern of palatal wound-healing after graft harvesting under the administration of aminoguanidine (AG), an AGE inhibitor, or N-phenacylthiazolium bromide (PTB), a glycated cross-link breaker. Full-thickness palatal excisional wounds (5.0 x 1.5 mm(2)) were created in 72 Sprague-Dawley rats. The rats received daily intraperitoneal injections of normal saline (control), AG, or PTB and were euthanized after 4 to 28 days. The wound-healing pattern was assessed by histology, histochemistry for collagen matrix deposition, immunohistochemistry for AGE and the AGE receptor (RAGE), and the expression of RAGE, as well as inflammation- and recovery-associated genes. In the first 14 days following AG or PTB treatments, wound closure, re-epithelialization, and collagen matrix deposition were accelerated, whereas AGE deposition, RAGE-positive cells, and inflammation were reduced. RAGE and tumor necrosis factor-alpha were significantly down-regulated at day 7, and heme oxygenase-1 was persistently down-regulated until day 14. The levels of vascular endothelial growth factor, periostin, type I collagen, and fibronectin were all increased at day 14. In conclusion, anti-AGE agents appeared to facilitate palatal wound-healing by reducing AGE-associated inflammation and promoting the recovery process.

bFGF Promotes the Migration of Human Dermal Fibroblasts under Diabetic Conditions through Reactive Oxygen Species Production via the PI3K/Akt-Rac1- JNK Pathways

“…In addition, advanced glycation end-products (AGEs) mediate the activation of ROS and blocks wound healing through impairing dermal fibroblast proliferation and migration 42. Anti-AGE agents appear to facilitate wound healing by reducing AGE-associated inflammation and promoting the recovery process 43, 44. However, there is no difference in ROS production between normal glucose and high glucose group in our study.…”

Light‐Emitting Diode Irradiation Promotes Donor Site Wound Healing of the Free Gingival Graft

“…A total of 72 male Sprague‐Dawley rats (250 to 300 g) were used. In each rat, bilateral 5 × 1.5 mm 2 palatal wounds parallel to the gingival margin of maxillary molars with 1 mm distance were created using a double‐bladed scalpel (1.5 mm interblade distance) with two number‐15 surgical blades as described previously, 21 and the LED light irradiation was started immediately after the wound creation. Animals were divided equally into three groups: 1) the control (Ctrl) group (no LED light irradiation); 2) the low‐dose (LD) group (daily 10 J/cm 2 LED light irradiation); and 3) the high‐dose (HD) group (daily 20 J/cm 2 LED light irradiation).…”

pH‐Responsive Hydrogel With an Anti‐Glycation Agent for Modulating Experimental Periodontitis

“…All of the images were acquired using a digital imaging system §§ and quantified using imaging software ‖‖ as described previously 21 . The lamina propria of the gingiva was selected as the region of interest.…”

“…All of the images were acquired using a digital imaging system § § and quantified using imaging software ii as described previously. 21 The lamina propria of the gingiva was selected as the region of interest. The inflammatory cell infiltration was quantified using the slides stained with H&E at ·400 magnification, and the result is presented as the number of inflammatory cells per square millimeter area.…”