In this paper, results of the flight‐testing of an unmanned aerial vehicle (UAV) flight control system are presented. APC‐4 “SkyGuide” autonomous navigation and control system, designed and developed by the research team of the Department of Avionics and Control at Rzeszów University of Technology, has been tested. Properties of this flight control system, as well as selected results of the in‐flight tests conducted on board of the PZL‐110 “Koliber” aircraft, are presented. Results obtained confirm that design assumptions of the navigation and control system and research methodology have been appropriate and APC‐4 autopilot can be used on UAVs board.