Software for muscle fibre type classification and analysis

Karen, Petr; Števanec, Milan; Smerdu, Vika; Cvetko, Erika; Kubínová, Lucie; Eržen, Ida

doi:10.4081/ejh.2009.e11

Cited by 19 publications

(6 citation statements)

References 31 publications

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“…The manual analysis of the different sections is laborious and time consuming, that is why several authors developed semi-automatic image analysis softwares. 15,16 …”

Section: Introductionmentioning

confidence: 99%

A simplified immunohistochemical classification of skeletal muscle fibres in mouse

et al. 2014

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The classification of muscle fibres is of particular interest for the study of the skeletal muscle properties in a wide range of scientific fields, especially animal phenotyping. It is therefore important to define a reliable method for classifying fibre types. The aim of this study was to establish a simplified method for the immunohistochemical classification of fibres in mouse. To carry it out, we first tested a combination of several anti myosin heavy chain (MyHC) antibodies in order to choose a minimum number of antibodies to implement a semi-automatic classification. Then, we compared the classification of fibres to the MyHC electrophoretic pattern on the same samples. Only two anti MyHC antibodies on serial sections with the fluorescent labeling of the Laminin were necessary to classify properly fibre types in Tibialis Anterior and Soleus mouse muscles in normal physiological conditions. This classification was virtually identical to the classification realized by the electrophoretic separation of MyHC. This immuno-histochemical classification can be applied to the total area of Tibialis Anterior and Soleus mouse muscles. Thus, we provide here a useful, simple and time-efficient method for immunohistochemical classification of fibres, applicable for research in mouse.

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“…The manual analysis of the different sections is laborious and time consuming, that is why several authors developed semi-automatic image analysis softwares. 15,16 …”

Section: Introductionmentioning

confidence: 99%

A simplified immunohistochemical classification of skeletal muscle fibres in mouse

et al. 2014

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“…Average minimal muscle fiber diameters, average fiber cross-sectional areas, and numerical proportions of muscle fibers were calculated for each individual sample (right and left side of the costal diaphragm and vastus lateralis) by software for muscle fiber type classification and analysis [10]. Statistical analysis was performed with the statistical package SYSTAT version 5.0 for Windows.…”

Section: Methodsmentioning

confidence: 99%

Size and Proportions of Slow-Twitch and Fast-Twitch Muscle Fibers in Human Costal Diaphragm

Meznarič

Cvetko

2016

BioMed Research International

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Smaller diaphragmatic motor unit potentials (MUPs) compared to MUPs of limb muscles lead to the hypothesis that diaphragmatic muscle fibers, being the generators of MUPs, might be also smaller. We compared autopsy samples of costal diaphragm and vastus lateralis of healthy men with respect to fibers' size and expression of slow myosin heavy chain isoform (MyHC-1) and fast 2A isoform (MyHC-2A). Diaphragmatic fibers were smaller than fibers in vastus lateralis with regard to the mean minimal fiber diameter of slow-twitch (46.8 versus 72.2 μm, p < 0.001), fast-twitch (45.1 versus 62.4 μm, p < 0.001), and hybrid fibers (47.3 versus 65.0 μm, p < 0.01) as well as to the mean fiber cross-sectional areas of slow-twitch (2376.0 versus 5455.9 μm2, p < 0.001), fast-twitch (2258.7 versus 4189.7 μm2, p < 0.001), and hybrid fibers (2404.4 versus 4776.3 μm2, p < 0.01). The numerical proportion of slow-twitch fibers was higher (50.2 versus 36.3%, p < 0.01) in costal diaphragm and the numerical proportion of fast-twitch fibers (47.2 versus 58.7%, p < 0.01) was lower. The numerical proportion of hybrid fibers did not differ. Muscle fibers of costal diaphragm have specific characteristics which support increased resistance of diaphragm to fatigue.

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“…We identified fibre types from the series of variously stained sections and estimated their numerical and area fraction as well as diameter applying our software (23). On average, 220 fibres were analysed per muscle (2·640 totally).…”

Section: Methodsmentioning

confidence: 99%

Wearing of complete dentures reduces slow fibre and enhances hybrid fibre fraction in masseter muscle

Cvetko

Karen

Eržen

2012

J of Oral Rehabilitation

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Edentulous conditions and use of complete dentures alter the function of jaw muscles, which is presumably reflected in the myosin heavy chain (MyHC) isoform composition. This study is the first dealing with MyHC isoforms expression in edentulous persons with the aim to clarify to which extent the decreased functional load following teeth loss contributes to the changed muscle phenotype during ageing. We analysed MyHC expression in old masseter muscle at decreased and full functional load by comparing age-matched edentulous and dentate subjects. Edentulous subjects had upper and lower complete dentures. Dentate subjects had at least 24 natural teeth in continuous dental arches with two molars present in each quadrant and normal intermaxillary relationship. The adaptive response to the reduced masticatory load was lower numerical and area proportion of MyHC-1 expressing fibres and higher numerical proportion of hybrid fibres in edentulous compared with dentate subjects with no significant difference in the proportion of MyHC-neo-expressing fibres between both groups. We conclude that the observed differences in the proportion of fibre types between denture wearers and dentate subjects cannot be ascribed to degenerative changes intrinsic to the ageing muscle, but to functional differences in muscle activity and to morphological alterations of stomatognathic system accompanying the complete teeth loss.

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Software for muscle fibre type classification and analysis

Cited by 19 publications

References 31 publications

A simplified immunohistochemical classification of skeletal muscle fibres in mouse

A simplified immunohistochemical classification of skeletal muscle fibres in mouse

Size and Proportions of Slow-Twitch and Fast-Twitch Muscle Fibers in Human Costal Diaphragm

Wearing of complete dentures reduces slow fibre and enhances hybrid fibre fraction in masseter muscle

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