In this study, the effect of environmentally friendly R290 refrigerant on the performance of the refrigerator was investigated, instead of R404A refrigerant, which negatively affects global warming and is the most frequently used gas in supermarket cabinets. The boundary conditions in the refrigeration cycle were designed to be -10°C evaporation and +45°C condensation, and the performance of the system referenced in the TS EN ISO 23953-2 standard was experimentally compared for both refrigerants. The temperature and energy consumption values of the M-packs, whose location and quantity are specified in the relevant standard, have been evaluated in terms of the cooling performance of the cabinet. As a result of the study, the heat removal performance of the system using R290 fluid in the condenser showed better results compared to R404A. According to the relevant standard, the system using R290 is in the C and D classes in the energy consumption evaluation.