Soil bacteria Cupriavidus sp. mediates the extracellular synthesis of antibacterial silver nanoparticles

Ameen, Fuad; Alyahya, Sami A.; Govarthanan, Muthusamy; Aljahdali, Nesreen; Al‐Enazi, Nouf M.; Alsamhary, Khawla; Alshehri, Wafa A.; Alwakeel, Suaad S.; Alharbi, Sulaiman Ali

doi:10.1016/j.molstruc.2019.127233

Cited by 151 publications

(38 citation statements)

References 33 publications

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“…Using different concentrations of nanoparticles, the IC50 value showed different values of (% cell viability) and (% cell inhibition) for the different cells’ lines depending on the time of incubation [ 25 , 26 , 27 ]. There are several biological bases of AgNPs synthesis, and the difference between the current prepared materials compared to the others is the source of the biological route [ 28 , 29 , 30 , 31 , 32 ]. Here, the difference between the current work and previous ones is that we intended to prepare AgNPs via the biological route using Ganoderma enigmaticum as well as Trametes ljubarskyi white rot fungus materials.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Biogenic synthesis and cytotoxic effects of silver nanoparticles mediated by white rot fungi

Gudikandula

Srileka

Charya

et al. 2021

Heliyon

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Silver nanoparticles (AgNPs) were successfully synthesized using silver nitrate via the biological route using the culture filtrate of Ganoderma enigmaticum as well as Trametes ljubarskyi white rot fungi materials at room temperature. The proposed synthetic technique was applied for the first time for AgNPs preparation via the biological route through a low-cost pathway, which considered as an adequate direction of preparation compared to the commercial methods. This study reports the in vitro cytotoxic effect of biologically synthesized AgNPs in disposing of the human lung cancer cell line (A549) and human breast cancer cell (MCF-7) by using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. In addition, the viability of the tested cell lines was tested after treatment for 24 h in the presence of the prepared nanoparticles. The obtained results indicated the reduced viability of cancer cell lines with improving concentrations of AgNPs (40–120 μg/mL) at 24 h. Furthermore, at 120 μg/mL concentration, the fungal nanoparticles showed substantial cytotoxic effects toward the treated cells. Consequently, the results designated that the biologically synthesized silver nanoparticles have effective behavior for treating A549 and MCF-7 cancer cells from the laboratory experiment approach; however, additional studies are required to validate these results in vivo models as anticancer agents depending on their cytotoxic activity.

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Section: Introductionmentioning

confidence: 99%

RETRACTED: Biogenic synthesis and cytotoxic effects of silver nanoparticles mediated by white rot fungi

Gudikandula

Srileka

Charya

et al. 2021

Heliyon

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“…AgNPs synthesized using A. baumannii were tested against β-lactams-, aminoglycosides-, and quinolones-resistant E. coli, P. aeruginosa, and K. pneumoniae, and the results showed that the nanoparticles were effective against those microbes with MIC values of 3.1, 1.56, and 3.1 µg/mL, respectively [60]. However, a concrete comparison with other study reports describing different levels of ZOIs and MICs is difficult due to the differences in procedures and concentrations used [29,42,57,59,61] and biological strains used [28,41,42,47,61]. Besides, the shape and size of the synthesized AgNPs may affect their antimicrobial activity against both Gram-positive and Gram-negative bacteria and fungi [62].…”

Section: Antimicrobial Activitymentioning

confidence: 94%

Endophytic Bacteria Enterobacter hormaechei Fabricated Silver Nanoparticles and Their Antimicrobial Activity

Monowar

Rahman²,

Bhore

et al. 2021

Pharmaceutics

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Antimicrobial resistance (AMR), one of the greatest issues for humankind, draws special attention to the scientists formulating new drugs to prevent it. Great emphasis on the biological synthesis of silver nanoparticles (AgNPs) for utilization in single or combinatorial therapy will open up new avenues to the discovery of new antimicrobial drugs. The purpose of this study was to synthesize AgNPs following a green approach by using an endophytic bacterial strain, Enterobacter hormaechei, and to assess their antimicrobial potential against five pathogenic and four multidrug-resistant (MDR) microbes. UV-Vis spectroscopy, fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX), and zeta potential (ζ) were used to characterize the synthesized AgNPs. Endophytic E. hormaechei-mediated AgNPs (Eh-AgNPs) were represented by a strong UV-Vis absorbance peak at 418 nm within 5 min, forming spherical and polydispersed nanoparticles in the size range of 9.91 nm to 92.54 nm. The Eh-AgNPs were moderately stable with a mean ζ value of −19.73 ± 3.94 mV. The presence of amine, amide, and hydroxyl functional groups was observed from FTIR analysis. In comparison to conventional antibiotics, the Eh-AgNPs were more effective against Bacillus cereus (ATCC 10876) and Candida albicans (ATCC 10231), exhibiting 9.14 ± 0.05 mm and 8.24 ± 0.05 mm zones of inhibition (ZOIs), respectively, while displaying effective inhibitory activity with ZOIs ranging from 10.98 ± 0.08 to 13.20 ± 0.07 mm against the MDR bacteria. Eh-AgNP synthesis was rapid and eco-friendly. The results showed that Eh-AgNPs are promising antimicrobial agents that can be used in the development and formulation of new drugs to curb the menace of antimicrobial resistance in pathogenic and MDR microbes.

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“…Hence the utilization of biological or green approach methods for developing nanomaterials play a vital role. 2 The biological synthesis incorporates use of plants, microbes, biomolecules and biopolymers etc. among the different strategies of biosynthesis, and plant mediated approaches have many potential applications due to eco-friendly, cost-effective, high stability, controlled nucleation and easy process.…”

Section: Introductionmentioning

confidence: 99%

One Pot Green Synthesis and Structural Characterisation of Bio-Silver Nanoparticles Using Prunus Persica L.

Shamina¹,

Suriyaraj

Senjiskhan³

2022

Int J Life Sci Pharm Res

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The Plant mediated green synthesis of nanoparticles has attracted more attention among the researchers due to their physicochemical and biological properties and their immense applications in environmental, biomedical, agriculture, textile, and energy storage. The biosynthesis of nanoparticles using plants provides more advantages such as easy operation, eco-friendly, utilization of non-hazardous chemicals, less temperature or energy usage, etc. when compared to physical and chemical synthesis methods. In the present study, we propose aqueous extracts from the endemic-medicinal fruit Prunus persica L. as an efficient bioproduct for the green synthesis of silver-nanoparticles (AgNPs). The biosynthesized AgNPs were characterized using UV-Visible spectroscopy, Fourier transforms Infrared spectroscopy (FTIR) High-Resolution Transmission electron microscopy (HR-TEM), Scanning electron microscopy, Atomic Force Microscopy (AFM), and X-ray diffractometer (XRD). HR-TEM gives the formation of monodispersed spherical shape with a mean diameter of 12.6 ± 3.8 nm. The SAED pattern of the nanoparticles showed that the particles were highly crystalline in nature. The purity of the synthesized AgNPs samples was studied by EDS and confirmed the presence of Ag elements corresponding to TEM images. The 2D and 3D structure images of the nanoparticle were studied and the average particle size calculation has been performed using NOVA-TX software in AFM. X-ray diffraction analysis showed that the particles were crystalline in nature with face-centered cubic structure. The antimicrobial efficacy was evaluated using the resazurin assay against both Escherichia coli and Bacillus subtilis bacteria. The antimicrobial assay of the silver nanoparticles showed higher activity against Bacillus subtilis than Escherichia coli. Results confirmed this protocol as rapid, simple, eco-friendly and alternative to conventional physical, and chemical methods.

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Soil bacteria Cupriavidus sp. mediates the extracellular synthesis of antibacterial silver nanoparticles

Cited by 151 publications

References 33 publications

RETRACTED: Biogenic synthesis and cytotoxic effects of silver nanoparticles mediated by white rot fungi

RETRACTED: Biogenic synthesis and cytotoxic effects of silver nanoparticles mediated by white rot fungi

Endophytic Bacteria Enterobacter hormaechei Fabricated Silver Nanoparticles and Their Antimicrobial Activity

One Pot Green Synthesis and Structural Characterisation of Bio-Silver Nanoparticles Using Prunus Persica L.

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