Soil bacterial functional diversity as influenced by cadmium, phenanthrene and degrade bacteria application

Jiang, Wei; Wang, Jijun; Tang, Jianshe; Hou, Feng; Lu, Yitong

doi:10.1007/s12665-009-0153-y

Cited by 12 publications

(2 citation statements)

References 25 publications

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“…In our study, the POD activity was not affected by naphthalene treatment, which may be due to the formation of peroxidase in the biochemical oxidation reaction of biological respiration and organic matter. Several studies 6,30,31 have shown that naphthalene treatment did not significantly alter the soil respiration rate; thus, naphthalene treatment did not have an impact on peroxidase activity. Polyphenol oxidase activity is usually related to microbial community structure 2 .…”

Section: Discussionmentioning

confidence: 94%

Effects of naphthalene on soil fauna abundance and enzyme activity in the subalpine forest of western Sichuan, China

Liu¹,

Yang²,

Yang³

et al. 2019

Sci Rep

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Naphthalene has been widely used to study the role of soil fauna, but its potential non-target effects on soil enzyme activity remain unknown in subalpine forests. We added naphthalene for two years and determined the effect of such additions on the abundance of soil fauna and soil enzyme activities (β-glucosidase, cellobiohydrolase, invertase, peroxidase, polyphenol oxidase, N-acetyl-β-D-glucosaminidase, leucine arylamidase, urease, nitrate reductase and nitrite reductase) in a subalpine forest. Naphthalene could efficiently suppress the individual density and population of soil fauna in situ . The individual density and number of groups were decreased by 72.6–84.8% and 15.0–28.0%, respectively. Naphthalene significantly affected the activities of β-glucosidase, cellobiohydrolase, polyphenol oxidase, N-acetyl-β-D-glucosaminidase, leucine arylamidase and nitrite reductase and the activity increased in the first litter peak of naphthalene addition, and decreased at the later. The activities of β-glucosidase, cellobiohydrolase, polyphenol oxidase, peroxidase, N-acetyl-β-D-glucosaminidase, leucine arylamidase and nitrite reductase showed a negative correlation with the soil microbial PLFAs. Conversely, the activities of invertase, urease and nitrate reductase were positively correlated with the soil microbial PLFAs. Our results suggest that naphthalene is an effective method to reduce soil fauna in subalpine forest. The enzyme activity was influenced by soil fauna and microbial PLFAs.

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Section: Discussionmentioning

confidence: 94%

Effects of naphthalene on soil fauna abundance and enzyme activity in the subalpine forest of western Sichuan, China

Liu¹,

Yang²,

Yang³

et al. 2019

Sci Rep

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“…For the CLPP analysis, the carbon sources shown to be more informative regarding the effects of heavy metals on bacterial communities were selected. Other sources (such as D-xylose and D-cellobiose) identified in previous studies were also considered [50] – [52] . Canonical discriminant analysis (CDA) was performed on the selected carbon sources (β-Methyl-D-Glucoside, i-Erythritol, D-Mannitol, α-Cyclodextrin, N-Acetyl-D-Glucosamine, α-D-Lactose, and the two previously mentioned).…”

Section: Methodsmentioning

confidence: 99%

Analysis of the Diversity of Substrate Utilisation of Soil Bacteria Exposed to Cd and Earthworm Activity Using Generalised Additive Models

et al. 2014

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Biolog EcoPlates™ can be used to measure the carbon substrate utilisation patterns of microbial communities. This method results in a community-level physiological profile (CLPP), which yields a very large amount of data that may be difficult to interpret. In this work, we explore a combination of statistical techniques (particularly the use of generalised additive models [GAMs]) to improve the exploitation of CLPP data. The strength of GAMs lies in their ability to address highly non-linear relationships between the response and the set of explanatory variables. We studied the impact of earthworms (Aporrectodea caliginosa Savigny 1826) and cadmium (Cd) on the CLPP of soil bacteria. The results indicated that both Cd and earthworms modified the CLPP. GAMs were used to assess time-course changes in the diversity of substrate utilisation (DSU) using the Shannon-Wiener index. GAMs revealed significant differences for all treatments (compared to control -S-). The Cd exposed microbial community presented very high metabolic capacities on a few substrata, resulting in an initial acute decrease of DSU (i.e. intense utilization of a few carbon substrata). After 54 h, and over the next 43 h the increase of the DSU suggest that other taxa, less dominant, reached high numbers in the wells containing sources that are less suitable for the Cd-tolerant taxa. Earthworms were a much more determining factor in explaining time course changes in DSU than Cd. Accordingly, Ew and EwCd soils presented similar trends, regardless the presence of Cd. Moreover, both treatments presented similar number of bacteria and higher than Cd-treated soils. This experimental approach, based on the use of DSU and GAMs allowed for a global and statistically relevant interpretation of the changes in carbon source utilisation, highlighting the key role of earthworms on the protection of microbial communities against the Cd.

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Rapid Impact of Phenanthrene and Arsenic on Bacterial Community Structure and Activities in Sand Batches

Cébron

Arsène-Ploetze²,

Bauda³

et al. 2013

Microb Ecol

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The impact of both organic and inorganic pollution on the structure of soil microbial communities is poorly documented. A short-time batch experiment (6 days) was conducted to study the impact of both types of pollutants on the taxonomic, metabolic and functional diversity of soil bacteria. For this purpose sand spiked with phenanthrene (500 mg kg(-1) sand) or arsenic (arsenite 0.66 mM and arsenate 12.5 mM) was supplemented with artificial root exudates and was inoculated with bacteria originated from an aged PAH and heavy-metal-polluted soil. The bacterial community was characterised using bacterial strain isolation, TTGE fingerprinting and proteomics. Without pollutant, or with phenanthrene or arsenic, there were no significant differences in the abundance of bacteria and the communities were dominated by Pseudomonas and Paenibacillus genera. However, at the concentrations used, both phenanthrene or arsenic were toxic as shown by the decrease in mineralisation activities. Using community-level physiological profiles (Biolog Ecoplates™) or differential proteomics, we observed that the pollutants had an impact on the community physiology, in particular phenanthrene induced a general cellular stress response with changes in the central metabolism and membrane protein synthesis. Real-time PCR quantification of functional genes and transcripts revealed that arsenic induced the transcription of functional arsenic resistance and speciation genes (arsB, ACR3 and aioA), while no transcription of PAH-degradation genes (PAH-dioxygenase and catechol-dioxygenase) was detected with phenanthrene. Altogether, in our tested conditions, pollutants do not have a major effect on community abundance or taxonomic composition but rather have an impact on metabolic and functional bacterial properties.

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Soil bacterial functional diversity as influenced by cadmium, phenanthrene and degrade bacteria application

Cited by 12 publications

References 25 publications

Effects of naphthalene on soil fauna abundance and enzyme activity in the subalpine forest of western Sichuan, China

Effects of naphthalene on soil fauna abundance and enzyme activity in the subalpine forest of western Sichuan, China

Analysis of the Diversity of Substrate Utilisation of Soil Bacteria Exposed to Cd and Earthworm Activity Using Generalised Additive Models

Rapid Impact of Phenanthrene and Arsenic on Bacterial Community Structure and Activities in Sand Batches

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