The purpose of this investigation was to study the effects of urbanization and land‐use changes on vegetation and the concentration of carbon deposits in the Ziarat watershed, a tourism area, located in Golestan Province, in northern Iran. For this reason, we selected four land uses: forest, pasture, cultivated, and urban lands. Soil samples of from the 0‐ to 30‐cm depth were taken from a factorial nest design within two geographical aspects (north and west), and with five replications for each geographical aspect. Converting forest to urban and cultivated land uses resulted in considerable losses of organic carbon (OC) and the destruction of aggregates, which caused the mean weight diameter (MWD) of aggregates to decrease from 2.54 mm to 0.35 and 1.2 mm, respectively. Compared to forest land, the labile carbon (LC) was lower in urban, cultivated, and pasture land by 87, 66, and 54%, respectively. Also nonlabile carbon was lower in urban, cultivated, and pasture land by 76, 49, and 34%, respectively. Soil microbial respiration (SMR) was lower in urban, cultivated, and pasture land (0.09, 0.14, and 0.22 mg CO2 g–1 d–1, respectively) compared with forest land (0.22 mg CO2 g–1 d–1). Statistically significant positive correlations (P < 0.0001, with R = 0.73) between stable carbon and MWD were observed. This study quantified reductions in numerous soil quality parameters resulting from extensive land‐use changes and urbanization in Iran. Effective regional land‐use planning in Iran can integrate results of this study to facilitate land development with the fewest deleterious impacts to soil quality.