Soil propagule bank of ectomycorrhizal fungi associated with Masson pine (Pinus massoniana) grown in a manganese mine wasteland

Abstract

Ectomycorrhizal (ECM) fungal propagule bank could facilitate the regeneration and plantation of seedlings in disturbed area. In this study, Masson pine (Pinus massoniana) seedlings were used to bait the ECM fungal propagule bank buried in the soils collected from a manganese (Mn) mine wasteland and a non-polluted area in China. After 6-month growth, we found the seedlings grown in the Mn mine soil (Mn:3200 mg kg-1) did not display any toxicity symptoms. Based on morphotyping and ITS-PCR sequencing, we identified a total of 16 ECM fungal OTUs (operative taxonomic units) at 97% similarity threshold, among which 11 OTUs were recovered in the Mn mine soils and 14 in the non-polluted soil. Two soil types shared 9 OTUs and both of them were dominated by a Tylospora sp. Based on those soil propagule banks in Masson pine forests reported in previous, we speculated that some Atheliaceae species may be preferred in the soil propagule bank of some pine species, such as Masson pine. In addition, NMDS ordination displayed geographical position effects on soil propagule banks in five Masson pine forest from three sites at regional scale. In conclusion, Masson pine ECM seedlings could grow well in the Mn wasteland as a suitable tree species used for reforestation application in Mn mineland, in addition, Mn pollution did not alter the dominant ECM fungal species in the soil propagule banks.

Proteomic Insight into the Symbiotic Relationship of Pinus massoniana Lamb and Suillus luteus towards Developing Al-Stress Resistance

“…The symbiotic role of ectomycorrhizal fungi in Pinus species is also well known to naturally cope with the different biotic and abiotic stresses and increase immune response towards Al toxicity [ 5 , 44 , 57 , 58 , 59 , 60 ]. S. luteus , an ectomycorrhizal fungus, is well-known for its growth habit in adverse soil conditions, i.e., saline soils, drought, and metal toxicity [ 27 , 44 , 61 , 62 , 63 , 64 , 65 ]. S. luteus has been previously described to actively stimulate resistance towards high concentrations of Al by facilitating plant growth through reduced reactive oxygen species and accumulation of antioxidants [ 66 , 67 , 68 , 69 , 70 , 71 ].…”

Selection of efficient ectomycorrhizal fungi for improved growth, biomass and nutrient uptake of Shorea robusta seedlings

“…Sal seeds exhibit precocious germination from the onset, right before they detach from the parent tree (Phartyal et al 2013). Owing to their recalcitrant nature, sal seeds have to be sown immediately after shedding from the trees, and they require sufficient moisture along with appropriate potential mycorrhizal fungal symbionts to improve seedling establishment and performance (Murata et al 2017, Huang et al 2018, Kumar and Atri 2018). The post‐recruitment drought in summer before the shedding of sal seeds is another reason underlying the poor seedling regeneration, as reduction of hydration to 30% causes them to lose viability (Phartyal et al 2013).…”

“…The inoculation of roots with ECM fungi facilitates seedling growth and uptake of nutrients and water (Smith and Read 2008, Stuart and Plett 2019, Frank and Garcia 2021). In addition, ECM fungi protect the seedlings and plants from various biotic and abiotic stresses (Huang et al 2018, Guerrero‐Galan et al 2019, Zwiazek et al 2019). In Europe and North America, Quercus seedlings inoculated with ECM fungi are being used for the reforestation of contaminated and disturbed sites (Núñez et al 2006, Bermúdez‐Contreras et al 2022).…”

Effect of composite amendments on physicochemical properties of copper tailings repaired by herbaceous plants

“…In this case, the ecological restoration and management of mining areas gradually attract more attention (Valentín-Vargas et al 2014). At present, the direct pollution area caused by tailings in China alone has reached 100,000 hm 2 , and the indirectly polluted land is more than 1 million hm 2 (Huang et al 2018).…”