SrCu(1-x) CrxO2 nanoparticles (x=0.0, 0.6, 1.2, and 1.8) nanoparticles were synthesized using sol-gel processing and subjected to calcination at 650°C. The investigation delved into the influence of varying chromium concentrations on the structural, FTIR, electrical, and antimicrobial characteristics. The microstructure of the SrCu(1-x)CrxO2 nanoparticles (x=0.0, 0.6, 1.2, and 1.8) nanoparticles were analyzed through X-ray diffraction and transmission electron microscopy. Additionally, changes in chemical bonds were observed as the Cr content increased. The dielectric parameters exhibit an increase with higher Cr ratios. Additionally, there is a decrease in these parameters as the frequency rises, which could be attributed to the mobility of charged particles within the SrCu(1-x)CrxO2 nanostructure. Our investigation underscores the significant role of Cr incorporation in influencing both the dielectric properties and antimicrobial activity of Cr, emphasizing its relevance for diverse applications.