Solanum dulcamara L. (Bittersweet): Accumulation of Steroidal Alkaloids in the Plant and in Different in Vitro Systems

Ethylene treatment improves diosgenin accumulation in in vitro cultures of Dioscorea zingiberensis via up-regulation of CAS and HMGR gene expression

“…It was showed that diosgenin content was significantly and positively correlated with soluble protein (p < 0.01), as well as chlorophyll (p < 0.01). Ehmke and Eilert (1993) correlation between solasodine level and chlorophyll content in suspension cultures of Solanum dulcamara, which is in agreement with our results. Many studies show that transcriptional modulation of related genes is a common response to pathogens or elicitor signals.…”

Transformed Root Cultures of Solanum dulcamara L.: A Model for Studying Production of Secondary Metabolites

“…For example, Ehmke and Eilert (1993) did not detect any alkaloids in cell suspension cultures of S. dulcamara L. grown in dark. These alkaloids started to be synthesized when the cultures were grown in light and began to show some differentiation and rooting, but their level was not comparable to the original intact plant.…”

“…Several Solanum species including S. dulcamara were the subject of many in vitro manipulations, but attempts, which involved techniques like cell suspension and callus cultures failed to achieve the target. Secondary metabolites production, in general, needs a certain degree of tissue differentiation, something that is obviously lacking in those in vitro systems (Ehmke & Eilert, 1993;Rhodes et al, 1987). A more promising technique has been introduced as an alternative to the classical cell…”