Solid-Phase Capture of Pathogenic Bacteria by Using Gangliosides and Detection with Real-Time PCR

Desai, Prerak; Walsh, Marie K.; Weimer, Bart C.

doi:10.1128/aem.02601-07

Cited by 19 publications

(9 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, after addition of CTB to paired lactosomes, the magnitude in fluorescence was comparable to that of the negative controls (Figure 6b). These data indicate that MFGs, known to contain gangliosides (25) bind pathogenic toxins (30, 31) and hence are capable of intercepting pathogenic toxins specific to cholera. Lactosomes, although rich in phospholipids, do not contain appreciable concentrations of gangliosides and may provide biological protection through immuno-modulation rather than as a vector to thwart pathogen or toxin binding.…”

Section: Resultsmentioning

confidence: 79%

See 1 more Smart Citation

Lactosomes: Structural and Compositional Classification of Unique Nanometer-Sized Protein Lipid Particles of Human Milk

Argov-Argaman

Smilowitz

Bricarello³

et al. 2010

J. Agric. Food Chem.

View full text Add to dashboard Cite

Milk fat globules (MFG) are accepted primarily as triacylglycerol delivery systems. The identification of nanometer-sized lipid-protein particles termed ‘lactosomes’ that do not contain triacylglycerol raises the question of their possible functions. MFGs were isolated by slow centrifugation and lactosomes were isolated by ultracentrifugation at a density equivalent to plasma HDL (d > 1.063 g/ml) from human milk obtained from six volunteers at different lactation stages. Isolated lactosomes were analyzed and compared with MFGs for their size distribution, lipidome, proteome, and functional activity. Lactosomes from early milk—day 8, were found to be similar in size as those from mature milk >28 days averaging ~25 nm in diameter. In total, 97 non-redundant proteins were identified in the MFG and lactosome fractions, 46 of which were unique to the MFG fraction and 29 of which were unique to the lactosome fraction. The proteins identified in the lactosome and MFG fractions were enriched with proteins identified with immunomodulatory pathways. Unlike MFGs and GM1 laden reconstituted high- density lipoprotein (rHDL) that served as a positive control, lactosomal binding capacity to cholera toxin was weak. Lipidomic analyses found that lactosomes were devoid of triacylglycerol and gangliosides, unlike MFGs, but rich in a variety of phospholipid species. The data found differences in structure, composition and function between lactosomes and MFG suggesting these two particles are derived from different biosynthetic and/or secretory pathways. The results reveal a bioactive lipid-protein, nanometer-length scale particle that is secreted into milk not to supply energy to the infant, but to play unique, protective and regulatory roles.

show abstract

Section: Resultsmentioning

confidence: 79%

“…Lipid protein assemblies from plasma (i.e. lipoproteins) (43, 44) and from milk (45) exert protection against endotoxemia by binding and neutralizing bacterial-secreting toxins. In spite of their low abundance in milk as part of the MFG membrane, gangliosides play an important role in the prevention of infection and immunity (31, 46).…”

Section: Discussionmentioning

confidence: 99%

Lactosomes: Structural and Compositional Classification of Unique Nanometer-Sized Protein Lipid Particles of Human Milk

Argov-Argaman

Smilowitz

Bricarello³

et al. 2010

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Ganglioside composition of milk is implicated in binding and neutralizing various pathogens and their toxins such as enteropathogenic E. coli , C. jejuni , L. monocytogenes , Salmonella enterica (Typhi), S. sonnei , Helicobacter pylori vacuolating toxin, and cholera toxin (36, 105). The interaction between the membrane-associated ganglioside GM1 and cholera toxin protein subunit B is a well-studied system and is frequently employed for assessing the capabilities of any new analytical technique (20, 36). For example, gangliosides from pooled human milk exhibited the ability to reduce the binding of cholera toxin to GM1 by 93%, and structures from goat milk completely inhibited the binding of botulinum type A neurotoxin to trisialoganglioside-GT1b (67).…”

Section: Establishing Structure-function Relationships Of Human Milk mentioning

confidence: 99%

Breast Milk Oligosaccharides: Structure-Function Relationships in the Neonate

Smilowitz¹,

Lebrilla²,

Mills

et al. 2014

Annu. Rev. Nutr.

350

View full text Add to dashboard Cite

In addition to providing complete postnatal nutrition, breast milk is a complex biofluid that delivers bioactive components for the growth and development of the intestinal and immune systems. Lactation is a unique opportunity to understand the role of diet in shaping the intestinal environment including the infant microbiome. Of considerable interest is the diversity and abundance of milk glycans that are energetically costly for the mammary gland to produce yet indigestible by infants. Milk glycans comprise free oligosaccharides, glycoproteins, glycopeptides, and glycolipids. Emerging technological advances are enabling more comprehensive, sensitive, and rapid analyses of these different classes of milk glycans. Understanding the impact of inter- and intraindividual glycan diversity on function is an important step toward interventions aimed at improving health and preventing disease. This review discusses the state of technology for glycan analysis and how specific structure-function knowledge is enhancing our understanding of early nutrition in the neonate.

show abstract

“…( 23 , 24 ), Bacillus cereus ( 24 ), Campylobacter jejuni ( 25 ). The different forms of PCR based on their methods are real-time PCR ( 25 - 28 ), multiplex PCR, and reverse transcriptase PCR (RT-PCR) ( 23 ). RT-PCR is also found as multiplex RT-PCR ( 29 - 31 ) and real-time RT-PCR ( 29 , 32 - 34 ).…”

Section: Resultsmentioning

confidence: 99%

A Review of Approaches to Identify Enteric Bacterial Pathogens

Amani

Mirhosseini

Fooladi

2014

Jundishapur J Microbiol

View full text Add to dashboard Cite

Context:Diarrhea is a common disease across the world. According to WHO, every year about two billion cases of diarrhea are reported in the world. It occurs mainly in the tropical regions and is a main cause of morbidity and mortality, particularly in young children and adults.Evidence Acquisition:One of the major causes of diarrheal diseases is bacteria; detection of pathogenic bacteria is a global key to the prevention and identification of food-borne diseases and enteric infections (like diarrhea).Conclusions:Therefore, development of rapid diagnostic methods with suitable sensitivity and specificity is very important about this infectious disease. In this review, we will discuss some of the important diagnostic methods.

show abstract

Solid-Phase Capture of Pathogenic Bacteria by Using Gangliosides and Detection with Real-Time PCR

Cited by 19 publications

References 25 publications

Lactosomes: Structural and Compositional Classification of Unique Nanometer-Sized Protein Lipid Particles of Human Milk

Lactosomes: Structural and Compositional Classification of Unique Nanometer-Sized Protein Lipid Particles of Human Milk

Breast Milk Oligosaccharides: Structure-Function Relationships in the Neonate

A Review of Approaches to Identify Enteric Bacterial Pathogens

Contact Info

Product

Resources

About