Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plants

Gomez, Federico José Vicente; Hernández, Ismaél Gatica; Cerutti, Soledad; Silva, María Fernanda

doi:10.1016/j.microc.2015.05.013

Cited by 24 publications

(16 citation statements)

References 42 publications

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“…Gas chromatography coupled to mass spectrometry (GC-MS) has also been used to the qualitative and quantitative analysis of MLT, although it has improved sensitivity and specificity, the requirement for sample volatile derivatization presents a disadvantage for its application to MLT determination (Badria, 2002; Ragab et al, 2006). The sensitivity of capillary electrophoresis (CE) methods has also been reported to be similar to the high performance liquid chromatography (HPLC) approaches (Gomez et al, 2015). HPLC is the most commonly used technique for the analysis of MLT.…”

Section: Introductionmentioning

confidence: 98%

“…To overcome the difficulty, different sample purification techniques such as liquid–liquid extraction (LLE) (Arnao and Hernández-Ruiz, 2009; Huang and Mazza, 2011a,b; Byeon and Back, 2014), solid phase extraction (SPE) (Huang and Mazza, 2011a; Gomez et al, 2015), microextraction by packed sorbent (MEPS) (Mercolini et al, 2012), HPLC purification (Pape and Lüning, 2006) were commonly employed to enrich MLT from plant tissues. As the classical techniques, LLE and SPE are the most used methods for purification of MLT (Huang and Mazza, 2011a,b; Gomez et al, 2015). At the previous stage, a large volume of solvent and a large amount of plant tissues were needed when LLE was utilized.…”

Section: Introductionmentioning

confidence: 99%

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A Simple, Rapid Method for Determination of Melatonin in Plant Tissues by UPLC Coupled with High Resolution Orbitrap Mass Spectrometry

Yan

et al. 2017

Front. Plant Sci.

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Melatonin (MLT) was involved in regulating various stages of plant growth and development. However, due to the low concentration and complex matrixes of plant, the analysis of MLT is a challenging task. In this study, we developed a rapid and efficient method with simplified sample preparation by employing UPLC coupled with a high resolution Orbitrap mass spectrometry, and stable isotope-labeled MLT (MLT-d4) was first used as internal standard in the developed analytical method. In the developed method, we used one-step liquid–liquid extraction to purify the crude extracts both from shoot and root of rice for the analysis, which remarkably simplify the sample preparation process. The method exhibits high specificity and recovery yield (>96.4%). Good linearities were obtained for MLT ranging from 0.01 to 20 ng/ mL with determination coefficient (R2) of 0.9991. The limit of detection for MLT was 0.03 pg. Reproducibility of the method was evaluated by intra-day and inter-day measurements and the results showed that relative standard deviations were less than 7.2%. Moreover, MLT quantification was accomplished by using only 100 mg fresh plant tissues. Additionally, the established method was successfully applied to investigate the spatiotemporal distributions of MLT in rice under cadmium (Cd) stress condition. We found that the content of MLT in shoot and root of rice increased under Cd stress, suggesting that MLT would play a crucial role in modulating the responses to Cd stress in different plant tissues.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

A Simple, Rapid Method for Determination of Melatonin in Plant Tissues by UPLC Coupled with High Resolution Orbitrap Mass Spectrometry

Yan

et al. 2017

Front. Plant Sci.

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“…In turn, catechins and methylxanthines in green tea samples and coumarins in Aesculus hippocastanum and Heracleum sphondyliu [ 81 ]. Moreover, amino acids in black, jasmine, green tee [ 82 ], and indoleamines (melatonine) in Camelia sinensis and Tilia cordata [ 83 ]. The full characteristics of the method used are presented in the Table 5 .…”

Section: Resultsmentioning

confidence: 99%

Application of Capillary Electrophoresis to the Analysis of Bioactive Compounds in Herbal Raw Materials

2021

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The article is a summary of scientific reports from the last 16 years (2005–2021) on the use of capillary electrophoresis to analyze polyphenolic compounds, coumarins, amino acids, and alkaloids in teas or different parts of plants used to prepare aqueous infusions, commonly known as “tea” or decoctions. This literature review is based on PRISMA guidelines and articles selected in base of criteria carried out using PICOS (Population, Intervention, Comparison, Outcome, Study type). The analysis showed that over 60% of articles included in this manuscript comes from China. The literature review shows that for the selective electrophoretic separation of polyphenolic and flavonoid compounds, the most frequently used capillary electromigration technique is capillary electrophoresis with ultraviolet detection. Nevertheless, the use of capillary electrophoresis-mass spectrometry allows for the sensitive determination of analytes with a lower limit of detection and gives hope for routine use in the analysis of functional foods. Moreover, using the modifications in electrochemical techniques allows methods sensitivity reduction along with the reduction of analysis time.

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“…Table lists previous reports of analysis of indoles using liquid extraction method, together with the instrumental techniques, linear range, and limit of detection (LOD)/limit of quantitation (LOQ). Some other methods employ solid‐phase extraction (SPE) . Liquid extracting with organic solvents (including dispersive liquid–liquid micro‐extraction, DLLME) is a simple method but involves time consuming multi‐step procedures including an evaporation step.…”

Section: Introductionmentioning

confidence: 99%

Rapid measurement of indole levels in Brassica vegetables using one millilitre binary organic extraction solvent and capillary electrophoresis‐UV analysis

Suparman

Inpota

Phonchai

et al. 2020

Phytochemical Analysis

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Introduction Brassica vegetables contain high levels of indole compounds which have been found to provide health benefits, especially as cancer‐preventive agents. An efficient and rapid method using solvent extraction with capillary electrophoresis (CE) and ultraviolet (UV) detection was developed for the determination of four major indoles from four types of Brassica vegetables. Materials and Methods Freeze‐dried samples of four Brassica vegetables, i.e. broccoli, cauliflower, Chinese cabbage and cabbage, were selected. Hence, 1 mL of the binary solvent dimethylformamide (DMF)–methanol, 4:1 (v/v), was used for sample extraction. The extracts were diluted with the running buffer and directly analysed using CE with UV detection of four indole compounds. Results The binary solvent DMF–methanol, 4:1 (v/v) was selected from studies of the extraction efficiency of standard indoles spiked in ivy gourd (as the negative control sample) and using diphenylamine as the internal standard. Recovery was 80(±10)–120(±3)% for the four indoles: indole‐3‐carbinol (I3C), indole‐3‐acetonitrile (I3A), indole‐3‐acetic acid (IAA), and 3,3′‐diindolylmethane (DIM). For direct analysis suitable dilution of the extract with the running buffer was required. The linear range of the quantitation is 0.75–25.0 μg/mL, limit of detection (LOD) of 0.14–0.52 μg/mL and r2 > 0.998. The amount of indole in the Brassica vegetables are in the order I3C > > IAA, I3A > DIM. Conclusion A rapid method for extraction and quantitation of four indoles in four Brassica vegetables using CE with UV detection was developed. It has the potential as an efficient technique for generating data for use in agricultural and nutritional studies.

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Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plants

Cited by 24 publications

References 42 publications

A Simple, Rapid Method for Determination of Melatonin in Plant Tissues by UPLC Coupled with High Resolution Orbitrap Mass Spectrometry

A Simple, Rapid Method for Determination of Melatonin in Plant Tissues by UPLC Coupled with High Resolution Orbitrap Mass Spectrometry

Application of Capillary Electrophoresis to the Analysis of Bioactive Compounds in Herbal Raw Materials

Rapid measurement of indole levels in Brassica vegetables using one millilitre binary organic extraction solvent and capillary electrophoresis‐UV analysis

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