Solid-phase extraction of ochratoxin A from wine based on a binding hexapeptide prepared by combinatorial synthesis

Evaluation of Ochratoxin Recognition by Peptides Using Explicit Solvent Molecular Dynamics

“…However, the magnitudes of K D-OTA-Pred were ~2 orders of magnitude higher than the K D-OTA-Expt estimates, and could be attributed to variations in experimental settings or the force field limitations. Experimentally derived binding data were obtained using routine biophysical techniques (like SPR, HPLC) under environmental conditions that were unique to each study design [13,14,15]. In such studies, the in-solution dynamics may have been compromised by the routine experimental practices like peptide conjugation, bio-immobilization, or ochratoxin tagging, such as by conjugation with a larger enzyme, that are essential for the detection and monitoring of the analytes [6,11].…”

“…Because of these benefits, a variety of platforms based on immobilized peptides and aptamers have been developed against OTA during the last decade [6,10,11,12]. For example, Giraudi et al were the first to report a hexapeptide receptor, raised using phage display libraries, for OTA targeting [13]. Heurich et al later reported peptide receptors with improved affinity to OTA by identifying an octamer and a 13-mer using computational modeling approaches and validated the peptides using gravimetric techniques [14].…”

Mycotoxins in fruits and their processed products: Analysis, occurrence and health implications

“…Thus, over the last few years, many efforts have been made to substitute antibodies with combinatorial peptides, low mass synthetic ligands, aptamers and molecularly imprinted polymers. Among these different approaches, well-designed combinatorial peptides have great potential as capturing agents and allow good recoveries (>95%) at limits of quantification of 2 g/L [77]. Confirmation of the presence of OTA in various matrices has frequently been achieved by LC-MS [78][79][80].…”

Aflatoxin Measurement and Analysis

“…The principle of solid phase extraction (SPE) columns is a variation of chromatographic techniques that uses a solid phase and a liquid phase to isolate one, or one type, of analyte from a solution. The columns contain different packing materials, ranging from silica gel, C-18 (octadecylsilane), florisil, phenyl, aminopropyl, ion exchange materials, both anionic and cationic, and molecular imprinted polymers (Giraudi et al, 2007;Jornet et al, 2000;Mateo et al, 2002;Vatinno et al, 2008;Yu & Lai, 2010;Zambonin et al, 2001). The generally procedure is to load the sample into column, retain the analyte, wash away impurities, and then elute the analyte.…”