Solid phase microextraction (SPME) analysis of volatile compounds produced by in vitro shoots of Lantana camara L. under the influence of auxins and cytokinins

Affonso, Vanessa Ribeiro; Bizzo, H. R.; Lima, Sharon Santos de; Esquibel, Maria Apparecida; Sato, Alice

doi:10.1590/s0103-50532007000800009

Cited by 18 publications

(13 citation statements)

References 4 publications

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“…These data agree with those reported for leaf volatiles from A. zerumbet cultured in medium containing cytokinin, where data evidenced a large number of hydrocarbons in leaf volatiles when KIN and BAP were applied . Effects of cytokinins on the level of volatile compounds have been reported in tissue cultures of medicinal plants (Affonso et al, 2007). However, in this study, the addition of cytokinin did not improve methyl salicylate production in vitro cultures of P. paniculata, conversely a reduction in its content was observed in growing concentrations of BAP (Figure 3).…”

Section: Resultscontrasting

confidence: 50%

“…Tissue culture techniques have some advantages as physical and biological manipulation of medicinal plants under standardized conditions, use of elicitors and plant growth regulators to optimize bioactive compounds and subculturing of elite genotypes (Affonso et al, 2007;Victório et al, 2008;Victório et al, 2009;. The purpose of this study was to evaluate in vitro organogenesis of P. paniculata under standardized conditions and volatiles production using the growth regulator BAP (6-benzylaminopurine).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Polygala paniculata: um recurso de salicilato de metila produzido por cultura de tecidos vegetais

Victório

Carriço²,

Lage³

2011

Rev. Ceres

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RESUMOPolygala paniculata: um recurso de salicilato de metila produzido por cultura de tecidos vegetais O estabelecimento de cultura in vitro de Polygala paniculata L. permitiu a propagação rápida de matéria-prima vegetal sob condições assépticas e padronizadas. As plantas in vitro foram submetidas à extração e destilação simultâneas (EDS) e a composição química do óleo essencial foi analisada por CG/DIC e CG/EM. Foram avaliados os efeitos de BAP (6-benzilaminopurina) sobre o desenvolvimento in vitro e produção de voláteis em P. paniculata. A principal característica do óleo essencial consistiu na presença marcante de salicilato de metila. Plantas cultivadas em meio controle MS (Murashige & Skoog) produziram mais do que 80% de salicilato, enquanto o uso de BAP, reduziu a produção de salicilato de metila a 71% e 21% para as concentrações de 2 e 4 mg L -1 , respectivamente. A cultura de tecidos de P. paniculata pode ser indicada como fonte de salicilato de metila. The establishment of Polygala paniculata L. tissue culture allowed a rapid propagation of raw plant material under aseptic and standardized conditions. Plantlets were subjected to simultaneous distillation-extraction (SDE) and volatiles composition analyzed by GC/FID and GC/MS. The effect of BAP (6-benzylaminopurine) on in vitro development and volatiles production was evaluated. The main feature of volatile compounds was the significant amount of methyl salicylate. Plantlets cultured on control MS (Murashige & Skoog) medium showed more than 80% of salicylate, while the addition of BAP at 2 and 4 mg L -1 concentrations reduced methyl salicylate production to 71% and 21%, respectively. Tissue culture of P. paniculata proved to be a potential source of methyl salicylate. Palavras

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Section: Resultscontrasting

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Polygala paniculata: um recurso de salicilato de metila produzido por cultura de tecidos vegetais

Victório

Carriço²,

Lage³

2011

Rev. Ceres

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“…Currently, volatile organic compounds (VOCs) have received special attention from the scientific community, as they have a wide range of biological and pharmacological uses and there are many publications related to the emission of volatiles [12]. Affonso et al [13] treated Lantana camara plants with high concentrations (0.44 and 4.4 µmol/L) of BA and showed an increase in the production of volatile compounds detected by solid phase microextraction (SPME). These include myrcene, α-phellandrene, α-copaene, trans-caryophyllene and β-gurjunene.…”

Section: Introductionmentioning

confidence: 99%

Influence of Benzyladenine on Metabolic Changes in Different Rose Tissues

Ibrahim

Agarwal

et al. 2018

Plants

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Two modern rose varieties, Floribunda and Hybrid Tea, were used to analyze and identify metabolic changes after foliar application with benzyladenine (BA). Volatile organic compounds (VOCs) as metabolites were detected. Two pairs of doses of BA, at 11.16 and 17.87 mg/cm2, and 7.17 and 12.26 mg/cm2 were applied to the foliage of Hybrid Tea and Floribunda, respectively. Sampling time was optimized and treatment duration was 4 weeks. After treatment, the volatiles from the treated and untreated control roses were extracted using headspace solid-phase microextraction (HS-SPME) technology by three-phase fiber 50/30 µm divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) and analyzed by gas chromatography (GC) coupled with a flame ionization detector (FID), and with mass spectrometry (GC-MS).The results showed that BA and its dose rate led to metabolic changes of treated roses in comparison with untreated controls. The number of VOCs extracted and detected from leaves, stem, rhizosphere and whole plants from the two rose varieties at doses rate of 17.87 and 12.26 mg/cm2 were 43, 65, 40 and 68 compounds for each plant material, respectively, for both rose varieties. Whilst the VOCs extracted and detected from both rose varieties for leaves, stem, rhizosphere and whole plants were 38, 61, 34 and 66 compounds for each plant material, respectively. The results demonstrate that some volatiles, such as 4-Heptyn-2-ol, Phenyl methyl ether and 3-Methyl-apopinene, increased with increasing doses of BA; these compounds are aroma chemicals with a very powerful smell. This study shows that BA treatments can have a significant effect on metabolite changes in different rose tissues. This method could be applied to other floriculture plants.

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“…On another study by Marotti et al (1996) who found that the high economic value of basil oil is attributable to the existence of phenyl propanoids, like eugenol, chavicol and their derivatives or terpenoids like monoterpen alchole linalool, methyl cinnamate, and limonene. Affonso et al (2007) mentioned that BA has also been used to enhance the growth and expansion of aromatic plants. Utilization Lantana camara L. plants with BA at 0.44 and 4.4 μmol/L raised volatile organic ingredient (VOC) production, which was detected by solid phase micro-extraction.…”

Section: Volatile Oil Componentsmentioning

confidence: 99%

“…BA has additionally been used to enhance the growth and development of aromatic flowers. Treating Lantana camara L. flora with BA increased unstable natural compound production as volatile oil contents (Affonso et al, 2007). Similarly, Zielińska et al (2011) indicated that the production of volatile oil contents elevated with the utility of various PGRs, such as indole-3acetic acid (IAA), thidiazuron, kinetin, and BA, when applied to Agastache rugosa flowers grown in vitro.…”

Section: Introductionmentioning

confidence: 99%

Effect of Benzyl Adenine, Indole Acetic Acid and Gibberellic Acid on Vegetative Growth, Chemical Constituents and Volatile Oil Attributes of Sweet Basil Plants

Nazmy

2020

Egyptian Journal of Horticulture

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B ENZYL ADENINE, indole acetic acid and gibberellic acid at different concentrations were sprayed to study their effects on growth, chemical components and volatile oils of sweet basil in 2018 and 2019 seasons Plant height, number of leaves/plant, average leaf area, and herb fresh and dry weights/ plant greatly improved with the applied treatments especially with IAA treatments followed by GA 3 treatments. However, the number of lateral branches/plant and stem diameter were increased with BA treatments especially with 10 ppm than 5 ppm. Generally, the second cut was superior in the studied vegetative growth parameters than the first cut. The highest values of N, P, K, Fe, and Mn were recorded with BA at 10 ppm, whereas higher Zn values were recorded with BA at 5 ppm. Active ingredients including total phenols, total flavones, total chlorophyll, and L-ascorbic acid greatly increased with IAA at 50 or 100 than the other treatments or the control. However, volatile oil percentage and volatile oil content/plant were high in IAA at 50 or 100 ppm followed by GA3 at 200 ppm. The second cut was superior to the first cut in promoting volatile oil percentage and volatile oil content/ plant in both studied seasons. The main components in volatile oil were 1,8-cineole, linalool, á-trans-bergamotene, germacrene D and á-muurolol. The highest values of linalool, the main component in the volatile oil of sweet basil (50.90%) was recorded by IAA at 100 ppm.

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Solid phase microextraction (SPME) analysis of volatile compounds produced by in vitro shoots of Lantana camara L. under the influence of auxins and cytokinins

Cited by 18 publications

References 4 publications

Polygala paniculata: um recurso de salicilato de metila produzido por cultura de tecidos vegetais

Polygala paniculata: um recurso de salicilato de metila produzido por cultura de tecidos vegetais

Influence of Benzyladenine on Metabolic Changes in Different Rose Tissues

Effect of Benzyl Adenine, Indole Acetic Acid and Gibberellic Acid on Vegetative Growth, Chemical Constituents and Volatile Oil Attributes of Sweet Basil Plants

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