Solid-phase microextraction technology for in vitro and in vivo metabolite analysis

Zhang, Qi‐Hui; Zhou, Ling; Chen, Hua; Wang, Chong‐Zhi; Xia, Zhining; Yuan, Chun‐Su

doi:10.1016/j.trac.2016.02.017

Cited by 87 publications

(41 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The SPE technique has been used successfully for separating organic compounds from complex samples (Zhang et al, 2016;Otles and Kartal, 2016).…”

Section: Results Of α-Mangostin γ-Mangostin and Gartanin Analysis Inmentioning

confidence: 99%

Validation Analysis Methods of a-Mangostin, ?-Mangostin and Gartanin Mixture in Mangosteen (Garcinia mangostana L.) Fruit Rind Extract from West Java with HPLC

2017

J App Pharm Sci

View full text Add to dashboard Cite

The quantitative analysis methods for bioactive compounds in mangosteen pericarp have been reported. The purpose of this study is to validate the methods of analysis for α-mangostin, γ-mangostin, and gartanin in mangosteen rind extract derived from Bogor, Purwakarta, Subang and Tasikmalaya using High Performance Liquid Chromatography (HPLC) for routine analysis. The methods employed were Enduro C-18 reverse-phase (250 mm x 4.6 mm) column chromatography systems with Photo Diode Array detector 375 nm and acetonitrile: water containing 0.1% phosphoric acid (95:5) as the mobile phase at the flow rate of 1.0 mL/min and validation methods with parameters of linearity, limit of detection and quantification, precision, and accuracy. Mangosteen rind extracts were pre-treated with the technique of solid-phase extraction (SPE). The results of this study show that the validation results meet the requirements of the standard retention time of α-mangostin at 5.801 minutes, γ-mangostin at 4.707 minutes and gartanin at 5.290 minutes. The correlation coefficient (R) for each standard were 0.999, 0.999, and 0.999, respectively. The value of recovery for the α-mangostin, γ-mangostin, and gartanin were 100.32%, 102.31%, and 101.48%, respectively. The analysis shows that the levels of α -mangostin, γ-mangostin, and gartanin from Bogor are 13.87%, 8.28% and 10.44%, respectively. The results from mangosteen pericarp extract from Purwakarta are 10.07% for α-mangostin, 6.33% for γ-mangostin, and 8.76% gartanin. Mangosteen pericarp extract from Subang has concentrations of α-mangostin at 10.88%, γ-mangostin at 6.01%, and gartanin at 8.08%. The contents of α-mangostin, γ-mangostin, and gartanin from Tasikmalaya are 8.53%, 6.07%, 17.28% respectively. This study concludes that the methods are valid and can be used for routine analysis.

show abstract

“…The SPE technique has been used successfully for separating organic compounds from complex samples (Zhang et al, 2016;Otles and Kartal, 2016).…”

Section: Results Of α-Mangostin γ-Mangostin and Gartanin Analysis Inmentioning

confidence: 99%

Validation Analysis Methods of a-Mangostin, ?-Mangostin and Gartanin Mixture in Mangosteen (Garcinia mangostana L.) Fruit Rind Extract from West Java with HPLC

2017

J App Pharm Sci

View full text Add to dashboard Cite

show abstract

“…Major technological advancements in sample pretreatment methods and mass spectrometric methods in recent years has substantially facilitated plant hormone analysis [117,[145][146][147][148]. To date, MS studies have identified many plant hormones and their metabolites that might be involved in the molecular mechanisms and physiological functions of plant development, but the analysis at unicellular level has remained a challenge.…”

Section: Discussionmentioning

confidence: 99%

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

et al. 2020

View full text Add to dashboard Cite

Plant hormones are naturally occurring small molecule compounds which are present at trace amounts in plant. They play a pivotal role in the regulation of plant growth. The biological activity of plant hormones depends on their concentrations in the plant, thus, accurate determination of plant hormone is paramount. However, the complex plant matrix, wide polarity range and low concentration of plant hormones are the main hindrances to effective analyses of plant hormone even when state-of-the-art analytical techniques are employed. These factors substantially influence the accuracy of analytical results. So far, significant progress has been realized in the analysis of plant hormones, particularly in sample pretreatment techniques and mass spectrometric methods. This review describes the classic extraction and modern microextraction techniques used to analyze plant hormone. Advancements in solid phase microextraction (SPME) methods have been driven by the ever-increasing requirement for dynamic and in vivo identification of the spatial distribution of plant hormones in real-life plant samples, which would contribute greatly to the burgeoning field of plant hormone investigation. In this review, we describe advances in various aspects of mass spectrometry methods. Many fragmentation patterns are analyzed to provide the theoretical basis for the establishment of a mass spectral database for the analysis of plant hormones. We hope to provide a technical guide for further discovery of new plant hormones. More than 140 research studies on plant hormone published in the past decade are reviewed, with a particular emphasis on the recent advances in mass spectrometry and sample pretreatment techniques in the analysis of plant hormone. The potential progress for further research in plant hormones analysis is also highlighted.

show abstract

“…However, SPME is not a completely solvent-free technique when combined with HPLC because small volumes of the organic solvent are necessary for desorption of the analytes from the fiber prior to HPLC separation. Numerous reviews have presented the applications of SPME in the analysis of various samples, e.g., [91][92][93][94][95][96][97][98][99][100][101][102][103][104]. Table 2 illustrates some representative examples of SPME applications.…”

Section: Solid Phase Microextraction (Spme)mentioning

confidence: 99%

Green Approaches to Sample Preparation Based on Extraction Techniques

Aly

Górecki

2020

Molecules

View full text Add to dashboard Cite

Preparing a sample for analysis is a crucial step of many analytical procedures. The goal of sample preparation is to provide a representative, homogenous sample that is free of interferences and compatible with the intended analytical method. Green approaches to sample preparation require that the consumption of hazardous organic solvents and energy be minimized or even eliminated in the analytical process. While no sample preparation is clearly the most environmentally friendly approach, complete elimination of this step is not always practical. In such cases, the extraction techniques which use low amounts of solvents or no solvents are considered ideal alternatives. This paper presents an overview of green extraction procedures and sample preparation methodologies, briefly introduces their theoretical principles, and describes the recent developments in food, pharmaceutical, environmental and bioanalytical chemistry applications.

show abstract

Solid-phase microextraction technology for in vitro and in vivo metabolite analysis

Cited by 87 publications

References 85 publications

Validation Analysis Methods of a-Mangostin, ?-Mangostin and Gartanin Mixture in Mangosteen (Garcinia mangostana L.) Fruit Rind Extract from West Java with HPLC

Validation Analysis Methods of a-Mangostin, ?-Mangostin and Gartanin Mixture in Mangosteen (Garcinia mangostana L.) Fruit Rind Extract from West Java with HPLC

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

Green Approaches to Sample Preparation Based on Extraction Techniques

Contact Info

Product

Resources

About