Solid-phase UV spectrophotometric method for determination of ciprofloxacin

Pascual-Reguera, M. I.; Parras, Gertrudis Pérez; Dı́az, A Molina

doi:10.1016/j.microc.2004.01.003

Cited by 66 publications

(27 citation statements)

References 32 publications

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“…33 Before the drug release study, the PCL-CIP-NP microspheres were purified by resuspension with PBS (1 M, pH 7.4) and centrifuged three times. A linear relationship between concentration and absorbance at 270 nm of CIP in PBS was detected with a correlation coefficient of ∼0.99.…”

Section: Determination Of Cipmentioning

confidence: 99%

Magnetically stimulated ciprofloxacin release from polymeric microspheres entrapping iron oxide nanoparticles

Sirivisoot¹,

Harrison²

2015

IJN

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To extend the external control capability of drug release, iron oxide nanoparticles (NPs) encapsulated into polymeric microspheres were used as magnetic media to stimulate drug release using an alternating magnetic field. Chemically synthesized iron oxide NPs, maghemite or hematite, and the antibiotic ciprofloxacin were encapsulated together within polycaprolactone microspheres. The polycaprolactone microspheres entrapping ciprofloxacin and magnetic NPs could be triggered for immediate drug release by magnetic stimulation at a maximum value of 40%. Moreover, the microspheres were cytocompatible with fibroblasts in vitro with a cell viability percentage of more than 100% relative to a nontreated control after 24 hours of culture. Macrophage cell cultures showed no signs of increased inflammatory responses after in vitro incubation for 56 hours. Treatment of Staphylococcus aureus with the magnetic microspheres under an alternating (isolating) magnetic field increased bacterial inhibition further after 2 days and 5 days in a broth inhibition assay. The findings of the present study indicate that iron oxide NPs, maghemite and hematite, can be used as media for stimulation by an external magnetic energy to activate immediate drug release.

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Section: Determination Of Cipmentioning

confidence: 99%

Magnetically stimulated ciprofloxacin release from polymeric microspheres entrapping iron oxide nanoparticles

Sirivisoot¹,

Harrison²

2015

IJN

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“…A survey of literature has revealed several analytical methods for the determination of CIP in pharmaceutical dosage form and biological fluids, including spectrophotometry, [5][6][7][8] spectrofluorimetry, 9-11) HPLC, 12-16) capillary electrophoresis 17,18) and HPTLC. 19) CIP in admixtures with MET and ampicillin has been determined by NMR.…”

Section: )mentioning

confidence: 99%

“…Since CIP has a reduced activity against anaerobic pathogens, CIP and MET are coformulated together in pharmaceutical dosage forms for the treatment of mixed aerobic/anaerobic infections. 4) A survey of literature has revealed several analytical methods for the determination of CIP in pharmaceutical dosage form and biological fluids, including spectrophotometry, [5][6][7][8] spectrofluorimetry, [9][10][11] HPLC, [12][13][14][15][16] capillary electrophoresis 17,18) and HPTLC. 19) CIP in admixtures with MET and ampicillin has been determined by NMR.…”

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confidence: 99%

Validated Spectrophotometric Methods for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in Tablets

Mahrouse

Elkady

2011

CHEMICAL & PHARMACEUTICAL BULLETIN

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Regular ArticleCiprofloxacin (CIP, Fig. 1a) [1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-(piperazinyl)-quinolone-3-carboxylic acid] belongs to the second generation of fluoroquinolone antibacterial agents. These quinolones are effective against Grampositive and Gram-negative bacteria through inhibiting bacterial DNA gyrase (topoisomerase), the enzyme responsible for maintaining the superhelical twists in the bacterial DNA, leading to inhibition of bacterial DNA synthesis.1) Similar to other fluoroquinolones, CIP has shown clinical efficacy in the treatment of a wide range of systemic infections such as urinary tract, respiratory, gastrointestinal and cutaneous infections. Because of its low toxicity, very wide spectrum of antibacterial effect and low ability to cause bacterial resistance, CIP has been widely used in clinical practice.2) Metronidazole (MET, Fig. 1b) is used as an antiprotozoal, antiamebic and antibacterial drug.3) It is chemically designated as 2-methyl-5-nitroimidazole-1-ethanol. Since CIP has a reduced activity against anaerobic pathogens, CIP and MET are coformulated together in pharmaceutical dosage forms for the treatment of mixed aerobic/anaerobic infections. 4)A survey of literature has revealed several analytical methods for the determination of CIP in pharmaceutical dosage form and biological fluids, including spectrophotometry, [5][6][7][8] spectrofluorimetry, 9-11) HPLC, 12-16) capillary electrophoresis 17,18) and HPTLC. 19) CIP in admixtures with MET and ampicillin has been determined by NMR. 20) Recently, HPLC methods either with fluorescence detection or coupled with mass spectrometry (LC/MS) for determination of ciprofloxacin in human plasma 21,22) have also been published. MET has been determined by several methods involving spectrophotometry [23][24][25][26] and HPLC. [27][28][29][30][31][32] MET in intravenous admixture with CIP was determined by first-derivative spectrophotometry 33) and LC. 34) Literature reports only one article for the simultaneous determination of CIP and MET in tablet form by the same authors of the present work. In this article, a reversed phase ion-pair HPLC and TLC densitometry have been adopted for the simultaneous determination of both drugs in tablets.35) However, due to lack of such equipments in many resources-limited countries and the high costs of HPLC grade solvents and columns, alternative methods are needed to facilitate and increase the speed of analysis, with relatively few costs. To the best of the authors' knowledge, no previous article concerning simultaneous spectrophotometric determination of the two drugs was published. Spectrophotometry continues to be very popular, because of its simplicity, versatility and low cost. Therefore, the aim of the present work was to develop and validate different UV spectrophotometric and chemometric techniques for the simultaneous determination of CIP and MET in tablets without previous separation. Both derivative and derivative ratio spectrophotometry are useful technique for resolving two overlapping...

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“…1 Quality control analyses in the pharmaceutical industry involve the determination of multiple parameters for both raw materials and end products. The analytical techniques that have been used in quality control analyses of FQs in pharmaceutical products are high performance liquid chromatography (HPLC), [2][3][4][5] UV spectrophotometry 6 and titrimetry. 7 Within the context of capillary electrophoresis (CE), some methodologies have been described for FQs analysis in pharmaceutical formulations.…”

Section: Introductionmentioning

confidence: 99%

Validation of a capillary zone electrophoresis method for the determination of ciprofloxacin, gatifloxacin, moxifloxacin and ofloxacin in pharmaceutical formulations

Faria¹,

Souza²,

Oliveira³

2008

J. Braz. Chem. Soc.

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Um método alternativo por eletroforese capilar de zona (CZE) para determinação de ciprofloxacina (CPFLX), gatifloxacina (GTFLX), moxifloxacina (MFLX) e ofloxacina (OFLX) foi validado. O sistema de eletrólito utilizado consistiu da mistura de 25 mmol L -1 de TRIS/ HCl e 15 mmol L -1 de tetraborato de sódio em meio aquoso resultando em pH 8,87. A análise foi realizada sob detecção direta por UV em 282 nm com tempo de análise de 3 min. Os parâmetros analíticos de validação avaliados foram: linearidade (r > 0,998), seletividade (comparação entre a inclinação da curva de calibração de padronização externa e curva de calibração de adição de padrão), repetitividade em área para amostras (RSD %: < 3,94% para CPFLX, < 3,87% para GTFLX, 1,30% para MFLX e < 1,88% para OFLX), precisão intermediária em área para amostras (RSD%: < 3,59% para CPFLX, < 3,09% para GTFLX, 2,67% para MFLX e < 2,25% para OFLX), exatidão (média da faixa de recuperação: 101,2% para CPFLX, 101,0% para GTFLX, 101,3% para MFLX e 99,9% para OFLX), limite de detecção (mg L -1 : 2,72 para CPFLX, 1,92 para GTFLX, 0,795 para MFLX e 1,05 para OFLX), limite de quantificação (mg L -1 : 9,06 para CPFLX, 6,40 para GTFLX, 2,65 para MFLX e 3,50 para OFLX) e robustez. Devido à simplicidade, seletividade, precisão, exatidão e rapidez, o método pode ser uma alternativa interessante para auxiliar o controle da qualidade dessas drogas na indústria farmacêutica.An alternative capillary zone electrophoresis (CZE) method for the determination of ciprofloxacin (CPFLX), gatifloxacin (GTFLX), moxifloxacin (MFLX) and ofloxacin (OFLX) through a simple aqueous electrolyte system consisting of 25 mmol L -1 of TRIS/ hydrochloride and 15 mmol L -1 of sodium tetraborate buffer mixture (pH 8.87) using direct UV detection at 282 nm within 3 min was validated. The analytical parameters of validation evaluated were: linearity (r > 0.998), selectivity (comparison between slope of the calibration curve of external standard and calibration curve of standard addition), repeatability in area for sample (RSD%: < 3.94% for CPFLX, < 3.87% for GTFLX, 1.30% for MFLX and < 1.88% for OFLX), intermediate precision in area for sample (RSD%: < 3.59% for CPFLX, < 3.09% for GTFLX, 2.67% for MFLX and < 2.25% for OFLX), accuracy (mean of recovery range: 101.2% for CPFLX, 101.0% for GTFLX, 101.3% for MFLX and 99.9% for OFLX), limit of detection (mg L -1 : 2.72 for CPFLX, 1.92 for GTFLX, 0.795 for MFLX and 1.05 for OFLX), limit of quantification (mg L -1 : 9.06 for CPFLX, 6.40 for GTFLX, 2.65 for MFLX and 3.50 for OFLX) and robustness. Due to its simplicity, selectivity, precision, accuracy and rapidity, the methodology can be an interesting alternative for quality assurance in the pharmaceutical industry of these drugs.

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Solid-phase UV spectrophotometric method for determination of ciprofloxacin

Cited by 66 publications

References 32 publications

Magnetically stimulated ciprofloxacin release from polymeric microspheres entrapping iron oxide nanoparticles

Magnetically stimulated ciprofloxacin release from polymeric microspheres entrapping iron oxide nanoparticles

Validated Spectrophotometric Methods for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in Tablets

Validation of a capillary zone electrophoresis method for the determination of ciprofloxacin, gatifloxacin, moxifloxacin and ofloxacin in pharmaceutical formulations

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Solid-phase UV spectrophotometric method for determination of ciprofloxacin

Cited by 66 publications

References 32 publications

Magnetically stimulated ciprofloxacin release from&nbsp;polymeric microspheres entrapping iron oxide nanoparticles

Magnetically stimulated ciprofloxacin release from&nbsp;polymeric microspheres entrapping iron oxide nanoparticles

Validated Spectrophotometric Methods for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in Tablets

Validation of a capillary zone electrophoresis method for the determination of ciprofloxacin, gatifloxacin, moxifloxacin and ofloxacin in pharmaceutical formulations

Contact Info

Product

Resources

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Magnetically stimulated ciprofloxacin release from polymeric microspheres entrapping iron oxide nanoparticles

Magnetically stimulated ciprofloxacin release from polymeric microspheres entrapping iron oxide nanoparticles