Solid-state fermentation for xylanase production by Thermoascus aurantiacus using response surface methodology

Souza, Miriam Coelho; Roberto, Inês Conceição; Milagres, Adriane M. F.

doi:10.1007/s002530051589

Cited by 81 publications

(10 citation statements)

References 14 publications

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“…After diafiltration, none of the examined chemicals significantly increased enzyme recovery (extraction efficiency) suggesting that sodium acetate buffer alone was most effective. In previous studies, acetate buffer at about pH 5 was used for enzyme extraction [29, 36]; however, since extraction buffer optimization was not the goal for these studies, effectiveness of acetate buffer was not justified on enzyme recovery and extraction.…”

Section: Resultsmentioning

confidence: 99%

“…This is in agreement with our finding in switchgrass amendment studies. Both sodium acetate buffer and water have been introduced as preferable buffers for xylanase extraction from SSF [29, 36, 38–40]; however, inhibition of enzymes by buffer components was not examined. It should be mentioned that fungal enzymes were used in those studies in contrast with present study where bacterial enzyme was produced, therefore, it is likely to observe some differences in the nature of the enzyme and consequently in the extraction optimization.…”

Section: Resultsmentioning

confidence: 99%

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Selection of Conditions for Cellulase and Xylanase Extraction from Switchgrass Colonized by Acidothermus cellulolyticus

Rezaei

Joh

Kashima

et al. 2011

Appl Biochem Biotechnol

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Solid-state fermentation has been widely used for enzyme production. However, secreted enzymes often bind to the solid substrate preventing their detection and recovery. A series of screening studies was performed to examine the role of extraction buffer composition including NaCl, ethylene glycol, sodium acetate buffer, and Tween 80, on xylanase and cellulase recovery from switchgrass. Our results indicated that the selection of an extraction buffer is highly dependent on the nature and source of the enzyme being extracted. While a buffer containing 50 mM sodium acetate at pH 5 was found to have a positive effect on the recovery of commercial fungal-derived cellulase and xylanase amended to switchgrass, the same buffer had a significant negative effect on enzyme extraction from solid fermentation samples colonized by the bacterium Acidothermus cellulolyticus. Xylanase activity was more affected by components in the extraction buffers compared to cellulase. This study demonstrated that extraction followed by diafiltration is important for assessing enzyme recovery from solid fermentation samples. Reduction in activity due to compounds present in the switchgrass extracts is reversible when the compounds are removed via diafiltration.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Selection of Conditions for Cellulase and Xylanase Extraction from Switchgrass Colonized by Acidothermus cellulolyticus

Rezaei

Joh

Kashima

et al. 2011

Appl Biochem Biotechnol

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“…It is well known that T. aurantiacus is an organism which can produce all three cellulases [1], [14], [15], [20], [29]. Specially the major component secreted by T. auranticaus , the endoglucanase exhibits high rates of substrate hydrolysis, superior thermostability, remarkable stability over a wide range of pH values and has tremendous commercial potential [3], [31], [7].…”

Section: Introductionmentioning

confidence: 99%

Purification and properties of an endoglucanase from Thermoascus aurantiacus

Dave

Sudhir

Subramanian

2015

Biotechnology Reports

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An Endo-cellulase was purified to homogeneity using ammonium sulfate precipitation, ion exchange and size exclusion chromatography from newly isolated strain of Thermoascus aurantiacus RBB-1. The recovery and purification fold were 13.3% and 6.6, respectively, after size exclusion chromatography. The purified cellulase has a molecular mass (M) of 35 kDa. Optimum temperature for the enzyme was found to be 70 °C and stability was upto 80 °C for 1 h. Along with higher stability at 80 °C, enzyme showed half lives of 192 h and 144 h at 50 and 70 °C respectively. The purified cellulase was optimally active at pH 4.0 and was stable over a broad pH range of 3.0–7.0. The enzyme purified showed apparent Km and Vmax values of 37 mg/ml and 82.6 U/min/mg protein respectively with higher salt tolerance of 10% for 1 h.

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“…Response surface methodology, described first by Box and Wilson (1951), an experimental strategy for seeking the optimum conditions for a multivariable system, is a much more efficient technique for optimization (Box and Hunter, 1978). This method had been successfully applied in the optimization of medium compositions (Roseiro, 1992;de O. Souza et al, 1999), conditions of enzymatic hydrolysis (Ma and Ooraikul, 1986), parameters of food preservation (King, 1983), and fermentation processes (Rosi et al, 1987;Kalil et al, 2000;Ramírez et al, 2001). A central composite experimental design was also used for the medium optimization reported here.…”

Section: Introductionmentioning

confidence: 99%

Enhanced production of elastase by Bacillus licheniformis ZJUEL31410: optimization of cultivation conditions using response surface methodology

Chen

Ruan

Zhang

et al. 2007

J. Zhejiang Univ. - Sci. B

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Sequential methodology based on the application of three types of experimental designs was used to optimize the fermentation conditions for elastase production from mutant strain ZJUEL31410 of Bacillus licheniformis in shaking flask cultures. The optimal cultivation conditions stimulating the maximal elastase production consist of 220 r/min shaking speed, 25 h fermentation time, 5% (v/v) inoculums volume, 25 ml medium volume in 250 ml Erlenmeyer flask and 18 h seed age. Under the optimized conditions, the predicted maximal elastase activity was 495 U/ml. The application of response surface methodology resulted in a significant enhancement in elastase production. The effects of other factors such as elastin and the growth factor (corn steep flour) on elastase production and cell growth were also investigated in the current study. The elastin had no significant effect on enzyme-improved production. It is still not clear whether the elastin plays a role as a nitrogen source or not. Corn steep flour was verified to be the best and required factor for elastase production and cell growth by Bacillus licheniformis ZJUEL31410.

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Solid-state fermentation for xylanase production by Thermoascus aurantiacus using response surface methodology

Cited by 81 publications

References 14 publications

Selection of Conditions for Cellulase and Xylanase Extraction from Switchgrass Colonized by Acidothermus cellulolyticus

Selection of Conditions for Cellulase and Xylanase Extraction from Switchgrass Colonized by Acidothermus cellulolyticus

Purification and properties of an endoglucanase from Thermoascus aurantiacus

Enhanced production of elastase by Bacillus licheniformis ZJUEL31410: optimization of cultivation conditions using response surface methodology

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