Solid-state fermentation of Bacillus thuringiensis var kurstaki HD-73 maintains higher biomass and spore yields as compared to submerged fermentation using the same media

Lima-Pérez, Jorge; López-Pérez, Marcos; Viniegra‐González, Gustavo; Loera, Octavio

doi:10.1007/s00449-019-02150-5

Cited by 22 publications

(11 citation statements)

References 39 publications

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“…The substrates after SSF could be utilized as feed additives directly without the treatment of separation and purification, which helps to improve the production efficiency. Meanwhile, Lima-Pérez et al reported that SSF of Bacillus thuringiensis maintained higher biomass and spore yields as compared to submerged fermentation using the same media [14]. Lu optimized the compositions of solid medium and found that wheat bran and soybean meal were the optimal carbon and nitrogen sources for B. subtilis Y31.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Bacillus subtilis Growth and Sporulation by Two-Stage Solid-State Fermentation Strategy

Zhao

et al. 2019

Processes

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Two-stage solid-state fermentation strategy was exploited and systematically optimized to enhance Bacillus subtilis growth and sporulation for increasing effective cell number in B. subtilis microbial ecological agents. The first stage focused on improving cell growth followed by the second stage aiming to enhance both cell growth and sporulation. The optimal fermentation condition was that temperature changed from 37 °C to 47 °C at a fermentation time of 48 h and Mn2+ content in medium was 4.9 mg MnSO4/g dry medium. Solid medium properties were improved by the optimal two-stage fermentation. HPLC results demonstrated that glucose utilization was facilitated and low-field nuclear magnetic resonance (LF-NMR) results showed that more active sites in medium for microbial cells were generated during the optimal two-stage fermentation. Moreover, microbial growth and sporulation were enhanced simultaneously during the second stage of fermentation through delaying microbial decline phase and increasing sporulation rate. As a result, effective cell number of B. subtilis reached 1.79 × 1010/g dry medium after fermentation for 72 h, which was 29.7% and 8.48% higher than that of conventional fermentation for 72 h and 48 h, respectively. Therefore, the optimal two-stage fermentation could increase the effective cell number of B. subtilis microbial ecological agents efficiently.

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Section: Introductionmentioning

confidence: 99%

Enhancement of Bacillus subtilis Growth and Sporulation by Two-Stage Solid-State Fermentation Strategy

Zhao

et al. 2019

Processes

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“…Cell lysis has also been associated with the production of esterases by Bacillus strains with lipolytic activity [ 59 , 60 , 61 , 62 ]. Therefore, while observing the death of vegetative cells after 10 h of cultivation in the presence of inulin and tributyrin, the sporulation process is likely to be initiated as a survival response [ 1 , 14 , 67 ]. Furthermore, Mallozzi and Handtke et al stated that sporulation is an alternative mechanism for B. pumilus cells to cope with exposure to non-glycolytic carbon sources and stress, which could be attributed to an adaptation process [ 62 , 63 , 65 ].…”

Section: Resultsmentioning

confidence: 99%

Physiological and Genomic Analysis of Bacillus pumilus UAMX Isolated from the Gastrointestinal Tract of Overweight Individuals

Reyes-Cortes

Lozano-Aguirre²,

Ponce‐Alquicira

2021

Microorganisms

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The study aimed to evaluate the metabolism and resistance to the gastrointestinal tract conditions of Bacillus pumilus UAMX (BP-UAMX) isolated from overweight individuals using genomic tools. Specifically, we assessed its ability to metabolize various carbon sources, its resistance to low pH exposure, and its growth in the presence of bile salts. The genomic and bioinformatic analyses included the prediction of gene and protein metabolic functions, a pan-genome and phylogenomic analysis. BP-UAMX survived at pH 3, while bile salts (0.2–0.3% w/v) increased its growth rate. Moreover, it showed the ability to metabolize simple and complex carbon sources (glucose, starch, carboxymethyl-cellulose, inulin, and tributyrin), showing a differentiated electrophoretic profile. Genome was assembled into a single contig, with a high percentage of genes and proteins associated with the metabolism of amino acids, carbohydrates, and lipids. Antibiotic resistance genes were detected, but only one beta-Lactam resistance protein related to the inhibition of peptidoglycan biosynthesis was identified. The pan-genome of BP-UAMX is still open with phylogenetic similarities with other Bacillus of human origin. Therefore, BP-UAMX seems to be adapted to the intestinal environment, with physiological and genomic analyses demonstrating the ability to metabolize complex carbon sources, the strain has an open pan-genome with continuous evolution and adaptation.

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“…The cultivation and sporulation of B. thuringiensis was compared in SmF and SSF by Lima-Pérez et al Interestingly, catabolic repression by glucose occurred in SmF with 25 gL −1 , but in SSF at 50 gL −1 . In SSF the sporulation started at just 15 h and gained the highest spore yield at 36 h [104]. LB (lysogeny broth) medium supplemented with raw potato flour was used for the endospore production of B. thuringiensis in a mixed cultivation of SmF and SSF.…”

Section: 6mentioning

confidence: 99%

Endospore production of Bacillus spp. for industrial use

Biermann,

Beutel

2023

Engineering in Life Sciences

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The increased occurrence of antibiotic resistance and the harmful use of pesticides are a major problem of modern times. A ban on the use of antibiotics as growth promoters in animal breeding has put a focus on the probiotics market. Probiotic food supplements are versatile and show promising results in animal and human nutrition. Chemical pesticides can be substituted by biopesticides, which are very effective against various pests in plants due to increased research. What these fields have in common is the use of spore‐forming bacteria. The endospore‐forming Bacillus spp. belonging to this group offer an effective solution to the aforementioned problems. Therefore, the biotechnological production of sufficient qualities of such endospores has become an innovative and financially viable field of research. In this review, the production of different Bacillus spp. endospores will be reviewed. For this purpose, the media compositions, cultivation conditions and bioprocess optimization methods of the last 20 years are presented and reflected.

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Solid-state fermentation of Bacillus thuringiensis var kurstaki HD-73 maintains higher biomass and spore yields as compared to submerged fermentation using the same media

Cited by 22 publications

References 39 publications

Enhancement of Bacillus subtilis Growth and Sporulation by Two-Stage Solid-State Fermentation Strategy

Enhancement of Bacillus subtilis Growth and Sporulation by Two-Stage Solid-State Fermentation Strategy

Physiological and Genomic Analysis of Bacillus pumilus UAMX Isolated from the Gastrointestinal Tract of Overweight Individuals

Endospore production of Bacillus spp. for industrial use

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