Solid substrate fermentation of agroresidues for value added products: the Malaysian experience

Sabaratnam, Vikineswary; Kumaran, K; Ling, Shi-Cheng; Dinesh, Nirmala; Shim, Y. L.

doi:10.1007/978-94-017-1711-3_25

Cited by 7 publications

(7 citation statements)

References 3 publications

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“…15 This residue can be used to produce value-added products. The production of biogas, 16 the production of bulk enzymes, 13 bioconversion to organic fertilizer, 17 use as an animal feed supplement, 18 and degradation of PCP 19,20 are among the potential uses or products available from spent mushroom compost.…”

Section: Introductionmentioning

confidence: 99%

Optimization of extraction of bulk enzymes from spent mushroom compost

Singh

Abdullah

Sabaratnam

2003

J of Chemical Tech & Biotech

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The profiling of ligninase, hemicellulase and cellulase of Pleurotus sajor-caju after inoculation of spawn in bags containing sawdust was done at monthly intervals for a period of 6 months. Xylanase (EC 3.2.1.8) was produced throughout the 6 months studied with the productivity range from 5.60 to 7.51 U g −1 . Cellulase (EC 3.2.1.4) and β-glucosidase (EC 3.2.1.21) productivities were highest at 4 months, producing 3.31 U g −1 and 121.13 U g −1 respectively. Laccase (EC 1.10.3.2) productivity was highest at 2 months with a value of 7.59 U g −1 . Lignin peroxidase (EC 1.11.1.14) productivity was highest at 5 months with a value of 206.20 U g −1 . Total soluble proteins were highest at 4 months with a value of 0.139 mg cm −3 . The profiling of lignin peroxidase in 5-month-old spent mushroom compost was monitored over a period of 10 months. It was observed that lignin peroxidase was produced throughout the period but productivity was variable. The average lignin peroxidase productivity ranged from 30 to 110 U g −1 . The activities of the enzymes extracted in tap water at pH 8.4 were comparable to that extracted in 50 mmol sodium citrate buffer at pH 4.8 and distilled water at pH 5.2 at 4• C using an incubator shaker at 200 rpm for 18 h. The optimum extraction time was 1 h using an incubator shaker at 4• C. When an incubator shaker was used, there was no significant difference in the recovery of xylanase, cellulase and laccase at different pH values at 4• C and 28 • C. No significant difference was observed in the recovery of β-glucosidase using an incubator shaker at different pH values at 4• C although the enzyme recovery was slightly higher at pH 8.12, with a value of 29.27 U g −1 . The optimum extraction of β-glucosidase was at pH 4 at room temperature using an incubator shaker. For the lignin peroxidase enzyme, the optimum pH for extraction was 6 at 4• C and pH 7 at room temperature using an incubator shaker at 200 rpm for 1 h. Homogenization for 8 min at 8000 rpm using tap water at pH 4 had an advantage over the use of the incubator shaker for the extraction as high titers of enzymes were recovered.

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Section: Introductionmentioning

confidence: 99%

Optimization of extraction of bulk enzymes from spent mushroom compost

Singh

Abdullah

Sabaratnam

2003

J of Chemical Tech & Biotech

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“…The culture types of fungi have been classified into solid-state fermentation (SSF) and submerged fermentation (SmF) based primarily on the type of substrate they use [ 5 6 ]. SSF utilizes solid substrates such as bran, bagasse, and paper pulp and is best suited for fermentation of fungi to obtain high yield of certain bioactive compounds.…”

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confidence: 99%

“…SSF utilizes solid substrates such as bran, bagasse, and paper pulp and is best suited for fermentation of fungi to obtain high yield of certain bioactive compounds. Recent advances have also suggested that SSF produces more stable and greater quantity of antibiotics than that obtained by SmF [ 6 ]. Spent mushroom substrate (SMS) is the post-harvest substrate and retains a variety of bioactive substances such as extracellular enzymes, antibiotics, secondary metabolites, and carbohydrates produced during mycelium and fruiting-body formation [ 7 ].…”

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confidence: 99%

Water Extract from Spent Mushroom Substrate of Hericium erinaceus Suppresses Bacterial Wilt Disease of Tomato

et al. 2015

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Culture filtrates of six different edible mushroom species were screened for antimicrobial activity against tomato wilt bacteria Ralstonia solanacearum B3. Hericium erinaceus, Lentinula edodes (Sanjo 701), Grifola frondosa, and Hypsizygus marmoreus showed antibacterial activity against the bacteria. Water, n-butanol, and ethyl acetate extracts of spent mushroom substrate (SMS) of H. erinaceus exhibited high antibacterial activity against different phytopathogenic bacteria: Pectobacterium carotovorum subsp. carotovorum, Agrobacterium tumefaciens, R. solanacearum, Xanthomonas oryzae pv. oryzae, X. campestris pv. campestris, X. axonopodis pv. vesicatoria, X. axonopodis pv. citiri, and X. axonopodis pv. glycine. Quantitative real-time PCR revealed that water extracts of SMS (WESMS) of H. erinaceus induced expressions of plant defense genes encoding β-1,3-glucanase (GluA) and pathogenesis-related protein-1a (PR-1a), associated with systemic acquired resistance. Furthermore, WESMS also suppressed tomato wilt disease caused by R. solanacearum by 85% in seedlings and promoted growth (height, leaf number, and fresh weight of the root and shoot) of tomato plants. These findings suggest the WESMS of H. erinaceus has the potential to suppress bacterial wilt disease of tomato through multiple effects including antibacterial activity, plant growth promotion, and defense gene induction.

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“…In a previous study, culture filtrates of Coprinus comatus and C. nuda showed that protective effects more than 80% differed from the 10% of the control, while the culture filtrate of L. edodes did show low protective effect less than 20% ( Chen and Huang, 2010 ). It was reported that SSF exhibited higher activity of diverse bioactive substances including antibiotics than that of submerged fermentation of liquid cultural type ( Subramaniyam and Vimala, 2012 ; Vikineswary et al, 1997 ). Therefore, the higher protective effect in SMS of L. edodes than that in its culture filtrate may be attributed to the similar cultural type to SSF.…”

Section: Resultsmentioning

confidence: 99%

Defense Response and Suppression of Phytophthora Blight Disease of Pepper by Water Extract from Spent Mushroom Substrate of Lentinula edodes

Kang

Min

Kwak

et al. 2017

The Plant Pathology Journal

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The spent mushroom substrate (SMS) of Lentinula edodes that was derived from sawdust bag cultivation was used as materials for controlling Phytophthora blight disease of pepper. Water extract from SMS (WESMS) of L. edodes inhibited mycelial growth of Phytophthora capsici, suppressed Phytophthora blight disease of pepper seedlings by 65% and promoted growth of the plant over 30%. In high performance liquid chromatography (HPLC) analysis, oxalic acid was detected as the main organic acid compound in WESMS and inhibited the fungal mycelium at a minimum concentration of 200 mg/l. In quantitative real-time PCR, the transcriptional expression of CaBPR1 (PR protein 1), CaBGLU (β-1,3-glucanase), CaPR-4 (PR protein 4), and CaPR-10 (PR protein 10) were significantly enhanced on WESMS and DL-β-aminobutyric acid (BABA) treated pepper leaves. In addition, the salicylic acid content was also increased 4 to 6 folds in the WESMS and BABA treated pepper leaves compared to water treated leaf sample. These findings suggest that WESMS of L. edodes suppress Phytophthora blight disease of pepper through multiple effects including antifungal activity, plant growth promotion, and defense gene induction.

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Solid substrate fermentation of agroresidues for value added products: the Malaysian experience

Cited by 7 publications

References 3 publications

Optimization of extraction of bulk enzymes from spent mushroom compost

Optimization of extraction of bulk enzymes from spent mushroom compost

Water Extract from Spent Mushroom Substrate of Hericium erinaceus Suppresses Bacterial Wilt Disease of Tomato

Defense Response and Suppression of Phytophthora Blight Disease of Pepper by Water Extract from Spent Mushroom Substrate of Lentinula edodes

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