Solubility and Conformation of Silk Fibroin Membrane

Abstract

Transparency and insolubility of eardrum patch against exudates are important for otolaryngological surgery. The author prepared silk fibroin (SF) films with various concentrations and temperature and then examined solubility and conformation of SF films. SF films were transparent regardless of the various preparation conditions. Although most SF films are soluble in 1X PBS solution at 37 o C for 1 h, the SF film with 3.4% with 60 o C was insoluble. Scanning electron microscopy (SEM) showed that the SF films have solid and smooth surface. Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC) results showed that the conformation of SF films was influenced by the preparation conditions including SF concentration and casting temperature. In conclusion, SF membrane with transparence and insolubility against exudates could be considered as eardrum patch resources.

Silk: history, obtaining, structure and properties of an old material in the development of new technologies

“…Therefore, they acquire the shape of the surface after the solvent present in the protein solution, in this case water, is evaporated under ambient conditions (dry casting). 2,4,105 In this way, non-patterned or patterned fibroin films can be achieved by changing only the substrate where the film is prepared. The choice of substrate will depend on the target application given that this film will "load" the cha-racteristics of the substrate.…”

Preparation and Characterization of Silk Beads for Protein Delivery System

“…The cocoon was degummed twice with 0.5% (Kweon et al, 2011). All other chemicals were used as received without any further purification.…”

Tetracycline-incorporated Silk Fibroin Films

“…The SF solution was obtained after dialysis against distilled water for 4 d, and then stored in refrigerator to use (Kweon et al, 2011). All chemicals were used as received without any further purification.…”