1989
DOI: 10.1002/bit.260340814
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Solubilization of soybean mitochondria in AOT/isooctane water‐in‐oil microemulsions

Abstract: The legend to Figure 1 1 should read as follows:The stability of Mitochondria-containing AOT micelles against temperature and water content (Wo). The AOT concentration is constant (100 mM). The water-in-oil microemulsion contained 97 micrograms of mitochondria1 proteins/mL of micellar solution.The legend to

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Cited by 2 publications
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“…Organic solubilization of proteins has also been achieved using the phase-transfer or extraction method, in which protein from an aqueous phase is extracted into an organic phase containing very low concentrations of a surfactant (Paradkar and Dordick, 1994). Much larger species, such as bacterial cells (Häring et al, 1985) and soy bean mitochondria (Hochkeoppler and Luisi, 1989), have been solubilized in reverse micelles as well, but required the addition of much more surfactant. Among the most commonly used systems for organic solubilization is Aerosol OT (AOT)/water/isooctane.…”
Section: Introductionmentioning
confidence: 99%
“…Organic solubilization of proteins has also been achieved using the phase-transfer or extraction method, in which protein from an aqueous phase is extracted into an organic phase containing very low concentrations of a surfactant (Paradkar and Dordick, 1994). Much larger species, such as bacterial cells (Häring et al, 1985) and soy bean mitochondria (Hochkeoppler and Luisi, 1989), have been solubilized in reverse micelles as well, but required the addition of much more surfactant. Among the most commonly used systems for organic solubilization is Aerosol OT (AOT)/water/isooctane.…”
Section: Introductionmentioning
confidence: 99%
“…Εκτός από ένζυμα και άλλα βιομόρια έχουν εγκλωβιστεί σε αντίστροφα μικκύλια όπως μεγάλα βιοπολυμερή π.χ. DNA [Goto et al, 2004], όργανα κυττάρων πχ μιτοχόνδρια [Hochkoeppler and Luisi, 1989], αλλά και ολόκληρα κύτταρα [Cinelli et al, 2006]. Γενικά η εισαγωγή του όρου «ένζυμο σε αντίστροφα μικκύλια» πραγματοποιήθηκε από τον Wells κάποια χρόνια αργότερα [Misiorowski and Wells, 1974].…”
Section: ένζυμα σε αντίστροφα μικκύλιαunclassified
“…Τα κύρια πλεονεκτήματα της μεθόδου αυτής παρουσιάζονται στον Πίνακα 5.4. [Hochkoeppler and Luisi, 1989;Goto et al, 2004;Cinelli et al, 2006;Anarbaev et al, 2009] σε αντίστροφα μικκύλια όπως περιγράφθηκε στην παράγραφο 3.3.6.1.2. Κατά τον σχηματισμό των αντίστροφων μικκυλίων το ένζυμο παραμένει διαλυτό στην υδατική μικροφάση ενώ τα μόρια των υποστρωμάτων και προϊόντων διαχέονται από και προς την οργανική φάση του συστήματος [Orlich and Schomacker, 2002;Dasgupta et al, 2005;Moniruzzaman et al, 2006].…”
Section: ενθυλάκωση (Encapsulation)unclassified