Soluble inhibitors/deactivators of cellulase enzymes from lignocellulosic biomass

Kim, Youngmi; Ximenes, Eduardo; Mosier, Nathan S.; Ladisch, Michael R.

doi:10.1016/j.enzmictec.2011.01.007

Cited by 422 publications

(306 citation statements)

References 45 publications

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“…The effect of improved enzymatic hydrolysis on the economics of the organosolv process was studied by setting sugar conversion yield 6 percentage points higher for all carbohydrates. On the other hand, due to enzyme inhibition and deactivation caused by soluble sugars, furfural, hydroxymethyl furfural, organic acids, and phenolics at higher solids contents (see Kim et al 2011), the conversion rates in industrial scale enzymatic hydrolysis could be significantly lower than those reported by Pan et al (2006). Therefore, a conversion of both cellulose and hemicelluloses to sugars of 80% was adopted as a more conservative scenario for the enzymatic hydrolysis.…”

Section: Technical Parameters Varied In Sensitivity Analysesmentioning

confidence: 99%

Economic Analysis of an Organosolv Process for Bioethanol Production

Kautto¹,

Park²,

Ragauskas³

et al. 2014

BioResources

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In a previous paper, conceptual process design, simulation, and mass and energy balances were presented for an organosolv process with a hardwood feed of 2350 metric tons (MT) per day and ethanol, lignin, furfural, and acetic acid production rates of 459, 310, 6.6, and 30.3 MT/day, respectively. In this paper, the investment and operating costs of the process and the minimum ethanol selling price (MESP) to make the process economically feasible were estimated. The total capital investment of the plant was approximately 720 million USD. Lignin price was found to affect the MESP considerably. With a base case lignin price of 450 USD/MT, the MESP was approximately 3.1 USD per gallon (gal). Higher lignin price of 1000 USD/MT was required to equal the MESP with the December 2013 ethanol market price (2.0 USD/gal). In addition to lignin price, the MESP was found to be strongly affected by feedstock, enzyme, and investment costs. Variations in feedstock and investment costs affected the MESP by approximately 0.2 and 0.5 USD/gal, respectively. Changing the enzyme dosage and price from base case estimate of 5270 USD/MT and 0.02 g/g cellulose to more conservative 3700 USD/MT and 0.06 g/g cellulose, respectively, increased the MESP by 0.59 USD/gal.

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Section: Technical Parameters Varied In Sensitivity Analysesmentioning

confidence: 99%

Economic Analysis of an Organosolv Process for Bioethanol Production

Kautto¹,

Park²,

Ragauskas³

et al. 2014

BioResources

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show abstract

“…Phenol concentrations depend on the biomass type, the pretreatment conditions and the biomass to water ratio. Although the level of inhibition is highly dependent on the type and concentration of phenol, these compounds are potent cellulase inhibitors even at low concentrations [10,[19][20][21]. Concentrations of phenolic compounds of 1.3 g/L were reported to strongly inhibit cellulases during the hydrolysis of microcrystalline cellulosic substrate [21].…”

Section: Steam Explosion Pretreatmentmentioning

confidence: 99%

“…Although the level of inhibition is highly dependent on the type and concentration of phenol, these compounds are potent cellulase inhibitors even at low concentrations [10,[19][20][21]. Concentrations of phenolic compounds of 1.3 g/L were reported to strongly inhibit cellulases during the hydrolysis of microcrystalline cellulosic substrate [21]. Similarly, García-Aparicio et al [22] found 50% cellulase inhibition in presence of prehydrolysate from steamexploded barley straw with 4.9 g/L total phenols.…”

Section: Steam Explosion Pretreatmentmentioning

confidence: 99%

Optimization of the laccase detoxification step in hybrid hydrolysis and fermentation processes from wheat straw by K. marxianus CECT 10875

Oliva-Taravilla¹,

Tomás‐Pejó²,

Demuez³

et al. 2016

Bioethanol

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The addition of laccase enzymes reduces the amount of phenols present in lignocellulosic pretreated materials and increases their fermentability. However, laccase addition in combination with cellulases reduces hydrolysis yields. In this work, hybrid hydrolysis and fermentation (HHF) configuration allowed overcoming the negative effect of laccase treatment on enzymatic hydrolysis. Furthermore, the effects of different laccase dosages, length of detoxification time and inoculum size on ethanol production were evaluated. In the evaluated configurations, the different laccase dosages did not show any significant effect on enzymatic hydrolysis. The lowest laccase dosage (0.5 IU/g DW) removed ~70% of total phenols which was enough to reach the highest ethanol production yields (~10 g/L) using K. marxianus CECT 10875. Shorter detoxification times and larger inoculum sizes had a positive impact on both ethanol production and volumetric productivity. These optimal detoxification conditions enable the fermentation of inhibitory slurries by reducing the overall time and cost of the process.

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“…7243 reaction conditions, and substrate pore surface area or substrate accessibility to cellulose (Lan et al 2013). It is also affected by the degraded products of hemicellulose and lignin (Mussatto et al 2008;Kim et al 2011). Irreversible loss of cellulases is observed with the non-specific binding of lignin, which subsequently reduces the efficiency of hydrolysis (Gao et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Optimization and Scale-Up of Enzymatic Hydrolysis of Wood Pulp for Cellulosic Sugar Production

Hu¹,

Zhu²,

Fang³

et al. 2016

BioResources

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With the decreased demand for pulp and paper worldwide, the reorganization of pulp and paper mills for cellulosic sugar production is possible. To maximize cellulosic sugar production from the wood pulp with minimum resources, the effects of pH, buffer system, temperature, enzyme loadings, pulp concentrations, and mixing modes on enzymatic hydrolysis were investigated, one factor at a time. Temperature played an important role in enzymatic hydrolysis. When the temperature was lower than 45 °C, the sugar production declined dramatically to almost half of the maximum value. Increasing enzyme dosage, increasing pulp concentration, and adding xylanase increased sugar production. The intermittent manual mixing mode generated higher concentrations of sugars and could be used for large-scale production. At pilot-scale, the diverted pulp for the pulping process was directly hydrolyzed without any treatment, and the residue after hydrolysis was hydrolyzed by adding fresh enzymes. This study provides insight on economically feasible enzymatic hydrolysis of wood pulp at large-scale cellulosic sugar production.

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Soluble inhibitors/deactivators of cellulase enzymes from lignocellulosic biomass

Cited by 422 publications

References 45 publications

Economic Analysis of an Organosolv Process for Bioethanol Production

Economic Analysis of an Organosolv Process for Bioethanol Production

Optimization of the laccase detoxification step in hybrid hydrolysis and fermentation processes from wheat straw by K. marxianus CECT 10875

Optimization and Scale-Up of Enzymatic Hydrolysis of Wood Pulp for Cellulosic Sugar Production

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