Soluble mannan and Î²-glucan inhibit the uptake of<i>Malassezia furfur</i>by human monocytic cell line, THP-1

Suzuki, Tatsuya; Ohno, Naohito; Ohshima, Yukio; Yadomae, Toshiro

doi:10.1111/j.1574-695x.1998.tb01169.x

Cited by 14 publications

(2 citation statements)

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“…Although recognition of Malassezia spp. products by TLRs and CLRs has been reported, the possible role of NLRs in innate immune responses to Malassezia spp. had never been investigated to date.…”

Section: Discussionmentioning

confidence: 99%

Malassezia yeasts activate the NLRP3 inflammasome in antigen‐presenting cells via Syk‐kinase signalling

Kistowska

Fenini

Janković

et al. 2014

Experimental Dermatology

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Although being a normal part of the skin flora, yeasts of the genus Malassezia are associated with several common dermatologic conditions including pityriasis versicolour, seborrhoeic dermatitis (SD), folliculitis, atopic eczema/dermatitis (AE/AD) and dandruff. While Malassezia spp. are aetiological agents of pityriasis versicolour, a causal role of Malassezia spp. in AE/AD and SD remains to be established. Previous reports have shown that fungi such as Candida albicans and Aspergillus fumigatus are able to efficiently activate the NLRP3 inflammasome leading to robust secretion of the pro-inflammatory cytokine IL-1β. To date, innate immune responses to Malassezia spp. are not well characterized. Here, we show that different Malassezia species could induce NLRP3 inflammasome activation and subsequent IL-1β secretion in human antigen-presenting cells. In contrast, keratinocytes were not able to secrete IL-1β when exposed to Malassezia spp. Moreover, we demonstrate that IL-1β secretion in antigen-presenting cells was dependent on Syk-kinase signalling. Our results identify Malassezia spp. as potential strong inducers of pro-inflammatory responses when taken up by antigen-presenting cells and identify C-type lectin receptors and the NLRP3 inflammasome as crucial actors in this process.

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Section: Discussionmentioning

confidence: 99%

Malassezia yeasts activate the NLRP3 inflammasome in antigen‐presenting cells via Syk‐kinase signalling

Kistowska

Fenini

Janković

et al. 2014

Experimental Dermatology

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“…We have long been working on the relationship between the structure and immunomodulating activity of b-glucans, using G. frondosa, [8][9][10] P. vesiculosa, 15) S. sclerotiorum, [11][12][13] S. commune, [21][22][23] O. lapidescens, 14) G. lucidum, 42) S. cerevisiae, 17) C. albicans, 16) M. furfur, 43,44) and S. crispa. 19) During this period, in a preclinical animal study, we found that the activity was significantly dependent on the molecular weight, degree of branching, and conformation.…”

Section: Discussionmentioning

confidence: 99%

Antitumor .BETA.-Glucan from the Cultured Fruit Body of Agaricus blazei.

Ohno

Furukawa

Miura

et al. 2001

Biological & Pharmaceutical Bulletin

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192

156

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An immunomodulating substance, a biological response modifier (BRM) or biotherapy is important for the treatment of cancer and infectious diseases. b-Glucan is a well-known BRM which is widely distributed in nature and used as a medicine and food. [1][2][3][4][5] The effects of "Lentinan" from Lentinus edodes and "Sonifilan" (SPG) from Schizophyllum commune in cancer therapy have been clinically proven.6,7) We have already developed b-glucans, GRN from Grifola frondosa, [8][9][10] SSG and TSG from Sclerotinia sclerotiorum, [11][12][13] OL-2 from Omphalia lapidescens, 14) PVS and PVG from Peziza vesiculosa, 15) CSBG from Candida spp., 16) OX-ZYM from a yeast cell preparation, zymosan, 17,18) and SCG from Sparaccis crispa. 19) We have also prepared several carboxymethyl, hydroxyethyl, sulfate, and polyol derivatives of the above b-glucans.20) In addition, we have analyzed the mechanism of b-glucan mediated immunopharmacological activity and identified the conformation dependent and independent activity. [21][22][23][24][25] These rather complicated relationships between structure and activity suggest the contribution of multiple receptor-ligand interactions in b-glucan mediated immunopotentiation.The incidence of cancer is gradually increasing and the spectrum of cancer-prone organs is changing each year. In addition to surgery, irradiation, and chemotherapy, immunotherapy is believed to be an important cancer therapy. 26)Immunotherapy and biotherapy include various approaches, such as BRMs, cytokines, lymphocyte transplantation, gene therapy, and herbal and alternative medicines. Clinical trials of these therapies are being widely carried out, and clinical evidence suggests their efficacy, although the precise mechanisms are still difficult to understand. The development of additional b-glucans is still needed for better biotherapy and to understand the molecular mechanisms.Agaricus blazei is a medical mushroom widely eaten and prescribed in Japan. There are various kind of health foods containing A. blazei and/or its extracts, but the molecular mechanisms of its action is far beyond complete understanding. The most anticipated pharmacological effect of A. blazei is modulation of the immune system against cancer. In the last decade, there have been several studies which focused on an antitumor polysaccharide from A. blazei, i.e., 1,6-b-glucan, glucan-protein complex, a-glucan, and heteroglycan. 27-34) Antitumor polysaccharides were screened not only in the fruitbody but also the liquid cultured medium and mycelium. One of the best characterized components, FIII-2-b, was prepared from the sodium hydroxide extract of the fruit body, and the structure was analyzed by methylation and NMR analyses. Contribution of a protein moiety to the activity of FIII-2-b was speculated by loss of the activity by partial formolysis. In the preliminary investigation, we have also determined that the major carbohydrate component of the fruit body of A. blazei is 1,6-b-glucan; however, we have already shown that a linear 1,6-b-glucan,...

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Lectinocytochemical detection of apoptotic murine leukemia L1210 cells

Bilyy

Stoika

2003

Cytometry Pt A

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Background Various cytomorphologic and biochemical markers of apoptosis are found in different compartments (plasma membrane, cytoplasm, nucleus, and mitochondria) of target cells. Although the plasma membrane is an easily accessible cellular compartment, relatively little is known about its changes during apoptosis. We investigated whether specific changes in the expression of plasma membrane glycoproteins take place during apoptosis and whether these changes could be used for a quantitative estimation of apoptosis. Methods Lectin cytochemical study of normal and apoptotic murine leukemia cells of the L1210 line was done. Horseradish peroxidase–labeled Laburnum anagyroides bark agglutinin, Phaseolus vulgaris agglutinin, Pisum sativum lectin (PSL), Ricinus communis agglutinin (RCA‐120; 120 kDa), Solanum tuberosum agglutinin, Triticum vulgaris lectin (wheat germ agglutinin), Viscum album agglutinin, Canavalia ensiformis lectin (concanavalin A), and Helix pomatia lectin were used to compare specific glycoprotein expressions in normal and apoptotic murine leukemia cells of the L1210 line sensitive (L1210) and resistant (L1210R) to apoptosis induction by cisplatin. Results The data demonstrated significantly increased binding of α‐D‐mannose–specific PSL lectin (P < 0.01) and β‐D‐galactose–specific RCA‐120 lectin (P < 0.001) by the apoptotic cells of the L1210 and L1210R lines in comparison with the intact cells. That binding was shown to be specific because it was blocked by the corresponding inhibitory sugars. Conclusions Lectins specific to α‐D‐mannose (PSL) and β‐D‐galactose (RCA‐120) can be used to distinguish between native and apoptotic murine leukemia L1210 cells and to quantitatively estimate apoptosis in a population of these cells. Cytometry Part A 56A:89–95, 2003. © 2003 Wiley‐Liss, Inc.

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Soluble mannan and Î²-glucan inhibit the uptake ofMalassezia furfurby human monocytic cell line, THP-1

Cited by 14 publications

References 20 publications

Malassezia yeasts activate the NLRP3 inflammasome in antigen‐presenting cells via Syk‐kinase signalling

Malassezia yeasts activate the NLRP3 inflammasome in antigen‐presenting cells via Syk‐kinase signalling

Antitumor .BETA.-Glucan from the Cultured Fruit Body of Agaricus blazei.

Lectinocytochemical detection of apoptotic murine leukemia L1210 cells

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