Soluble proteins in insect chemical communication

Pelosi, Paolo; Zhou, Jing‐Jiang; Ban, Liping; Calvello, M.

doi:10.1007/s00018-005-5607-0

Cited by 762 publications

(771 citation statements)

References 145 publications

(237 reference statements)

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“…Although CSPs have been identified in insect chemosensory sensilla, there is no clear evidence that they participate in olfaction or gustation. Nevertheless, several CSPs are highly expressed in the sensillar lymph and, in vitro, are capable to bind different components of the pheromonal blends (Pelosi et al, 2006). It is again worth emphasising that not all CSPs are restricted to chemosensory organs, and it has been postulated that they are involved in carbon dioxide detection, larval development and leg regeneration (reviewed in Wanner et al, 2004).…”

Section: Insect Chemosensory Gene Family Evolutionmentioning

confidence: 99%

Molecular evolution of the major chemosensory gene families in insects

2009

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Chemoreception is a crucial biological process that is essential for the survival of animals. In insects, olfaction allows the organism to recognise volatile cues that allow the detection of food, predators and mates, whereas the sense of taste commonly allows the discrimination of soluble stimulants that elicit feeding behaviours and can also initiate innate sexual and reproductive responses. The most important proteins involved in the recognition of chemical cues comprise moderately sized multigene families. These families include odorant-binding proteins (OBPs) and chemosensory proteins (CSPs), which are involved in peripheral olfactory processing, and the chemoreceptor superfamily formed by the olfactory receptor (OR) and gustatory receptor (GR) families. Here, we review some recent evolutionary genomic studies of chemosensory gene families using the data from fully sequenced insect genomes, especially from the 12 newly available Drosophila genomes. Overall, the results clearly support the birth-and-death model as the major mechanism of evolution in these gene families. Namely, new members arise by tandem gene duplication, progressively diverge in sequence and function, and can eventually be lost from the genome by a deletion or pseudogenisation event. Adaptive changes fostered by environmental shifts are also observed in the evolution of chemosensory families in insects and likely involve reproductive, ecological or behavioural traits. Consequently, the current size of these gene families is mainly a result of random gene gain and loss events. This dynamic process may represent a major source of genetic variation, providing opportunities for FUTURE specific adaptations.

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Section: Insect Chemosensory Gene Family Evolutionmentioning

confidence: 99%

Molecular evolution of the major chemosensory gene families in insects

2009

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“…Our data raises interesting question about the function of the expanded CSP genes in ants. Generally, insect CSPs are highly expressed in the sensillar lymph and, in vitro, capable of binding different components of pheromonal blends (Pelosi et al, 2006), but not all CSPs are restricted to chemosensory organs. Basal to ant-specific expansions is the orthologous CSP7, which in both L. humile and C. japonicus is conserved in the function of binding cuticular hydrocarbons used in ant chemical communication.…”

Section: Two Groups Of Genes With Different Evolutionary Patternsmentioning

confidence: 99%

Comparative genomics of chemosensory protein genes reveals rapid evolution and positive selection in ant-specific duplicates

2013

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Gene duplications can have a major role in adaptation, and gene families underlying chemosensation are particularly interesting due to their essential role in chemical recognition of mates, predators and food resources. Social insects add yet another dimension to the study of chemosensory genomics, as the key components of their social life rely on chemical communication. Still, chemosensory gene families are little studied in social insects. Here we annotated chemosensory protein (CSP) genes from seven ant genomes and studied their evolution. The number of functional CSP genes ranges from 11 to 21 depending on species, and the estimated rates of gene birth and death indicate high turnover of genes. Ant CSP genes include seven conservative orthologous groups present in all the ants, and a group of genes that has expanded independently in different ant lineages. Interestingly, the expanded group of genes has a differing mode of evolution from the orthologous groups. The expanded group shows rapid evolution as indicated by a high dN/dS (nonsynonymous to synonymous changes) ratio, several sites under positive selection and many pseudogenes, whereas the genes in the seven orthologous groups evolve slowly under purifying selection and include only one pseudogene. These results show that adaptive changes have played a role in ant CSP evolution. The expanded group of ant-specific genes is phylogenetically close to a conservative orthologous group CSP7, which includes genes known to be involved in ant nestmate recognition, raising an interesting possibility that the expanded CSPs function in ant chemical communication.

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“…The key function of OBPs is their ability to bind semiochemicals and measured mainly by fluorescence displacement binding assay (Pelosi et al, 2006;Zhou, 2010). The most widely used fluorescent probe is N-phenyl-1-naphthylamine (1-NPN), which has been employed in the binding of Drosophila LUSH (Zhou et al, 2004), the Locusta migratoris LmigOBP1-3 (Jiang et al, 2009;Yu et al, 2009), the social wasp Polistes dominulus OBP1 (Calvello et al, 2003) and Bombyx mori OBPs (Zhou et al, 2009;He et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Functional characterization and immunolocalization of odorant binding protein 1 in the lucerne plant bug, Adelphocoris lineolatus (GOEZE)

Wang

et al. 2011

Arch Insect Biochem Physiol

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In the insect phylum, the relationships between individuals and their environment are often modulated by chemical communication. Odorant binding proteins (OBPs) are widely and robustly expressed in insect olfactory organs and play a key role in chemosensing and transporting hydrophobic odorants across the sensillum lymph to the olfactory receptor neuron. In this study, a novel OBP gene (AlinOBP1) in the lucerne plant bug, Adelphocoris lineolatus was identified, cloned and expressed. Real-time PCR results indicated that the expression level of AlinOBP1 gene differed in each developmental stage (from first instar to adult) and was predominantly expressed in the antennae of adults. The expression level of AlinOBP1 was 1.91 times higher in male antennae than in female antennae. The binding properties of AlinOBP1 with 114 odorants were measured using a fluorescence probe, N-phenyl-1-naphthylamine (1-NPN), with fluorescence competitive binding. The results revealed that AlinOBP1 exhibits high binding abilities with two major putative pheromone components, ethyl butyrate and trans-2-hexenyl butyrate. In addition, it was observed that six volatiles released from cotton, octanal, nonanal, decanal, 2-ethyl-1-hexanol, b-caryophyllene and b-ionone also bind to AlinOBP1. Immunocytochemistry analysis showed that AlinOBP1 was expressed in the sensillum lymph of sensilla trichodica and sensilla basiconca. Our results demonstrate that AlinOBP1 may function as a carrier in the chemoperception of the lucerne plant bug. C 2011 Wiley Periodicals, Inc.

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Soluble proteins in insect chemical communication

Cited by 762 publications

References 145 publications

Molecular evolution of the major chemosensory gene families in insects

Molecular evolution of the major chemosensory gene families in insects

Comparative genomics of chemosensory protein genes reveals rapid evolution and positive selection in ant-specific duplicates

Functional characterization and immunolocalization of odorant binding protein 1 in the lucerne plant bug, Adelphocoris lineolatus (GOEZE)

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