Soluble starch synthase I: a major determinant for the synthesis of amylopectin in <i>Arabidopsis thaliana</i> leaves

Delvallé, David; Dumez, Sylvain; Wattebled, Fabrice; Roldán, Isaac; Planchot, Véronique; Berbezy, Pierre; Colonna, Paul; Vyas, Darshna; Chatterjee, Manash; Ball, Steven; Mérida, Ángel; d’Hulst, Christophe

doi:10.1111/j.1365-313x.2005.02462.x

Cited by 172 publications

(190 citation statements)

References 56 publications

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“…This mutant line was shown previously to contain 23% less starch in leaves at the end of the illumination period (Delvallé et al, 2005). The loss-of-function insert in this line was located in the intron of the genomic sequence, but a protein could not be detected.…”

Section: Infection Testmentioning

confidence: 89%

“…In the step for amylose formation, in addition to GBSS1, another gene involved in amylopectin synthesis, SS1, encoding a plastidial enzyme (Delvallé et al, 2005), was also up-regulated. Whereas Delvallé et al (2005) reported that SS1 mRNA levels increased at the onset of the light period, SS1 transcript levels were found by Smith et al (2004) to be almost constant during circadian rhythm.…”

Section: Discussionmentioning

confidence: 99%

“…It is synthesized mainly during photosynthesis in the light period buffering CO 2 fixation or in storage organs like tubers, bulbs, or seeds (Dennis and Blakeley, 2000). In recent years, numerous studies have been performed to increase knowledge of starch metabolism, including in the model plant Arabidopsis (Arabidopsis thaliana; Zeeman et al, 2002Zeeman et al, , 2004Siedlecka et al, 2003;Smith et al, 2004;Delvallé et al, 2005;Lloyd et al, 2005;Dumez et al, 2006). In most of these studies, plant leaf material of the wild type and mutant lines was used to investigate starch metabolism under different light regimes or altered sugar concentrations.…”

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confidence: 99%

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Starch Serves as Carbohydrate Storage in Nematode-Induced Syncytia

et al. 2007

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The plant parasitic nematode Heterodera schachtii induces specific syncytial feeding sites in the roots of Arabidopsis thaliana from where it withdraws all required nutrients. Therefore, syncytia have to be well supplied with assimilates and generate strong sinks in the host plant's transport system. Import mechanisms and consequent accumulation of sucrose in syncytia were described recently. In this work, we studied the starch metabolism of syncytia. Using high-performance liquid chromatography and microscopic analyses, we demonstrated that syncytia store carbohydrates by starch accumulation. Further, we monitored the expression of genes involved in the starch metabolic pathway by gene chip analysis and quantitative reverse transcription-PCR. Finally, we provide functional proof of the importance of starch synthesis for nematode development using T-DNA insertion lines. We conclude that syncytia accumulate starch as a carbohydrate buffer to compensate for changing solute uptake by the nematode and as long-term storage during juvenile development.

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Section: Infection Testmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Starch Serves as Carbohydrate Storage in Nematode-Induced Syncytia

et al. 2007

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“…Although these enzymes may have different activities depending on the plant species and tissues, they are all present in starchsynthesising cells. SSI appears to be primarily responsible for the synthesis of short glucan chains, as evidenced from SSI mutants in Arabidopsis [52]. Further investigation of the chain-length specificities of maize endosperm SSI has suggested that SSI prefers the shortest amylopectin chains as substrates [53].…”

Section: Starch Synthases (Sss)mentioning

confidence: 99%

Recent progress toward understanding the role of starch biosynthetic enzymes in the cereal endosperm

Li¹,

Powell²,

Gilbert

2017

Amylase

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Abstract:Starch from cereal endosperm is a major energy source for many mammals. The synthesis of this starch involves a number of different enzymes whose mode of action is still not completely understood. ADPglucose pyrophosphorylase is involved in the synthesis of starch monomer (ADP-glucose), a process, which almost exclusively takes place in the cytosol. ADPglucose is then transported into the amyloplast and incorporated into starch granules by starch synthase, starch-branching enzyme and debranching enzyme. Additional enzymes, including starch phosphorylase and disproportionating enzyme, may be also involved in the formation of starch granules, although their exact functions are still obscure. Interactions between these enzymes in the form of functional complexes have been proposed and investigated, resulting more complicated starch biosynthetic pathways. An overall picture and recent advances in understanding of the functions of these enzymes is summarized in this review to provide insights into how starch granules are synthesized in cereal endosperm.

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“…Genetic analysis suggests that these isoforms have differing roles in amylopectin synthesis. Starch synthase (SS) I is thought to be responsible for the synthesis of the short external chains of amylopectin (DP6-10; Delvalle et al 2005), whereas SSIIa is responsible for the synthesis of longer chains, from DP12-20. Elimination of this enzyme in barley , wheat (Yamamori et al 2000) and rice (Umemoto et al 2002) leads to a very characteristic phenotype involving reduced amylopectin external chain length, reduced granule gelatinization temperature and reduced starch swelling properties.…”

Section: Starch Biosynthesis and Functionalitymentioning

confidence: 99%

Genetic contributions to agricultural sustainability

Dennis

Ellis

Green

et al. 2007

Phil. Trans. R. Soc. B

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The current tools of enquiry into the structure and operation of the plant genome have provided us with an understanding of plant development and function far beyond the state of knowledge that we had previously. We know about key genetic controls repressing or stimulating the cascades of gene expression that move a plant through stages in its life cycle, facilitating the morphogenesis of vegetative and reproductive tissues and organs. The new technologies are enabling the identification of key gene activity responses to the range of biotic and abiotic challenges experienced by plants. In the past, plant breeders produced new varieties with changes in the phases of development, modifications of plant architecture and improved levels of tolerance and resistance to environmental and biotic challenges by identifying the required phenotypes in a few plants among the large numbers of plants in a breeding population. Now our increased knowledge and powerful gene sequence-based diagnostics provide plant breeders with more precise selection objectives and assays to operate in rationally planned crop improvement programmes. We can expect yield potential to increase and harvested product quality portfolios to better fit an increasing diversity of market requirements. The new genetics will connect agriculture to sectors beyond the food, feed and fibre industries; agri-business will contribute to public health and will provide high-value products to the pharmaceutical industry as well as to industries previously based on petroleum feedstocks and chemical modification processes.

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Soluble starch synthase I: a major determinant for the synthesis of amylopectin in Arabidopsis thaliana leaves

Cited by 172 publications

References 56 publications

Starch Serves as Carbohydrate Storage in Nematode-Induced Syncytia

Starch Serves as Carbohydrate Storage in Nematode-Induced Syncytia

Recent progress toward understanding the role of starch biosynthetic enzymes in the cereal endosperm

Genetic contributions to agricultural sustainability

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