Solute carrier protein family may involve in radiation-induced radioresistance of non-small cell lung cancer

Xie, Li; Song, Xianrang; J, Yu; Guo, Weijian; Wei, Ling; Li, Yanli; Wang, Xing-Wu

doi:10.1007/s00432-011-1050-9

Cited by 38 publications

(27 citation statements)

References 19 publications

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“…Deregulation of the amino acid transporter Slc7a7 is involved in multiple types of cancer, including gliobastoma, non-small cell lung cancer and multiple myeloma [60-62]. Studies conducted in recent years have demonstrated that diseases associated with Slc7a7 also include lysinuric protein intolerance and cystinuria [63].…”

Section: Discussionmentioning

confidence: 99%

Expression Profiling of Genes Modulated by Minocycline in a Rat Model of Neuropathic Pain

et al. 2014

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BackgroundThe molecular mechanisms underlying neuropathic pain are constantly being studied to create new opportunities to prevent or alleviate neuropathic pain. The aim of our study was to determine the gene expression changes induced by sciatic nerve chronic constriction injury (CCI) that are modulated by minocycline, which can effectively diminish neuropathic pain in animal studies. The genes associated with minocycline efficacy in neuropathic pain should provide insight into the etiology of neuropathic pain and identify novel therapeutic targets.ResultsWe screened the ipsilateral dorsal part of the lumbar spinal cord of the rat CCI model for differentially expressed genes. Out of 22,500 studied transcripts, the abundance levels of 93 transcripts were altered following sciatic nerve ligation. Percentage analysis revealed that 54 transcripts were not affected by the repeated administration of minocycline (30 mg/kg, i.p.), but the levels of 39 transcripts were modulated following minocycline treatment. We then selected two gene expression patterns, B1 and B2. The first transcription pattern, B1, consisted of 10 mRNA transcripts that increased in abundance after injury, and minocycline treatment reversed or inhibited the effect of the injury; the B2 transcription pattern consisted of 7 mRNA transcripts whose abundance decreased following sciatic nerve ligation, and minocycline treatment reversed the effect of the injury. Based on the literature, we selected seven genes for further analysis: Cd40, Clec7a, Apobec3b, Slc7a7, and Fam22f from pattern B1 and Rwdd3 and Gimap5 from pattern B2. Additionally, these genes were analyzed using quantitative PCR to determine the transcriptional changes strongly related to the development of neuropathic pain; the ipsilateral DRGs (L4-L6) were also collected and analyzed in these rats using qPCR.ConclusionIn this work, we confirmed gene expression alterations previously identified by microarray analysis in the spinal cord and analyzed the expression of selected genes in the DRG. Moreover, we reviewed the literature to illustrate the relevance of these findings for neuropathic pain development and therapy. Further studies are needed to elucidate the roles of the individual genes in neuropathic pain and to determine the therapeutic role of minocycline in the rat neuropathic pain model.

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Section: Discussionmentioning

confidence: 99%

Expression Profiling of Genes Modulated by Minocycline in a Rat Model of Neuropathic Pain

et al. 2014

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“…Data from another study also confirmed that SLC7A7 was upregulated at both mRNA and protein levels and that its overexpression was associated with poor prognosis in glioblastoma patients [28]. SLC7A7, involved in regulating amino acid transport, is closely linked to a variety of diseases [14][15][16][17]. A study focusing on ovarian cancers found that SLC7A7 may alter the influx/efflux of drugs from cells and thus modulate the chemotherapy response [16].…”

Section: Cellular Physiology and Biochemistrymentioning

confidence: 58%

“…High expression of SLC7A7 is related to the prognosis of malignant glioma [14] and multiple myeloma [15] and drug resistance in ovarian cancer [16]. Moreover, the high expression of SLC7A7 is also associated with the radiotherapy sensitivity of lung cancer [17]. Barilli et al found an increase in the mRNA expression of the SLC7A7 gene in human monocytes during the differentiation process of macrophages [18].…”

Section: Ji Et Al: Function Of Slc7a7 In T-allmentioning

confidence: 99%

Function of SLC7A7 in T-Cell Acute Lymphoblastic Leukemia

Yang

Wang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Y+LAT1 protein, encoded by the SLC7A7 gene (a member of the SLC7 family), forms the cationic amino acid transport system y+L (system y+L). This system transports cationic amino acids such as arginine and lysine out of the cell. Arginine, in particular, is critical for T-cell activation and function in the immune response. Methods: We analyzed the role of the SLC7A7 gene in the cellular activities of Jurkat cells, specifically the cell cycle and cell proliferation, apoptosis, migration, and invasion. Cell proliferation was assessed using the Cell Counting Kit-8. Apoptosis and the cell cycle were determined with a FACSCalibur flow cytometer. A Transwell chamber was used to measure cell invasion and migration. Results: The proliferative ability of Jurkat cells was not significantly altered by transfection with SLC7A7 overexpression vectors. However, SLC7A7 overexpression significantly decreased the percentage of apoptotic Jurkat cells (P = 0.007) but significantly increased the proportion of G1 phase cells (P = 0.029) and cell migration (P < 0.001) and invasion (P < 0.001). Knockdown of SLC7A7 increased the cell apoptosis rate (P = 0.006) but decreased the G1 phase ratio (P = 0.002) and cell migration (P < 0.001) and invasion (P < 0.001). Conclusions: SLC7A7 plays a significant role in the pathogenesis of T-cell acute lymphoblastic leukemia.

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“…Some previous studies have shown that the abnormal gene expression regulation of SLC7A7 is associated with many malignant tumors. The up-regulated gene expression of SLC7A7 is associated with radiation-induced radioresistance in non-small cell lung cancer (Xie et al, 2011). Cheng et al (2010) indicated that the overall expression of SLC7A7 is related to chemoresistance in ovarian cancer.…”

Section: Discussionmentioning

confidence: 99%

Association of three common single nucleotide polymorphisms of SLC7A7 with the development of glioma in a Chinese population

Zhang¹,

Shi²

2017

Genet. Mol. Res.

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ABSTRACT. Gliomas are brain tumors that can be seriously damaging to human health. The SLC7 family is involved in amino acid or peptide transportation. The relationship between SLC7A7 polymorphisms and the development of glioma has been reported previously by a few studies. Therefore, we performed a hospital based case-control study to investigate the association of three common SNPs (rs12888930, rs12436190, and rs2065134) of SLC7A7 with the development of glioma in a Chinese population. From January 2014 to December 2015, 122 patients with glioma and 252 individuals (controls) were recruited from the department of neurosurgery of Tangshan People's Hospital affiliated to North China University of Science and Technology. SLC7A7 rs12888930, rs12436190, and rs2065134 genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism method. Multiple logistic regression analysis showed that a significantly higher risk of glioma was harbored by the GG and AG + GG genotypes than by the AA genotype; OR (95%CI) was 2.24 (1.18-4.22) and 1.59 (1.01-2.60), respectively. However, no significant relationship was observed between SLC7A7 rs12888930 and rs2065134 and the risk of glioma. In conclusion, this study reports a significant association between SLC7A7 rs12436190 and the risk of glioma in a Chinese population.

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Solute carrier protein family may involve in radiation-induced radioresistance of non-small cell lung cancer

Cited by 38 publications

References 19 publications

Expression Profiling of Genes Modulated by Minocycline in a Rat Model of Neuropathic Pain

Expression Profiling of Genes Modulated by Minocycline in a Rat Model of Neuropathic Pain

Function of SLC7A7 in T-Cell Acute Lymphoblastic Leukemia

Association of three common single nucleotide polymorphisms of SLC7A7 with the development of glioma in a Chinese population

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