Solution casting of chitosan membranes for in vitro evaluation of bioactivity

Abstract

BackgroundConsiderable research is focusing on the surface modification of titanium implants for the treatment of orthopaedic tissue injuries to increase the success of orthopaedic fixations. Chitosan is one of the natural materials under investigation based on several favourable properties. Numerous techniques have been described for the preparation of chitosan membranes, including solution casting methods for the investigation of bioactivity before applying coatings onto potential titanium implants. Solution casting enables the easy in-house evaluation of chitosan membranes and allows for the selection of promising chitosan materials.ResultsWe present a method for the standardized and easily applied preparation of chitosan membranes by solution casting. This protocol is suitable for chitosan materials spanning a wide degree of deacetylation, being derived from different chitin sources and chitosan derivatives with novel properties. We detail the preparation and quality control methods in order to prepare membranes with favourable bioactivity, sustaining cell attachment and proliferation for extended culture periods.ConclusionsThe possibilities associated with the use of chitosan in tissue engineering applications are far from being exhausted and numerous challenges remain prior to successful translation into the clinics. Based on our experience, we have developed simple in-house methods for quality control of homogeneous membrane casting and early prediction of successful experimental outcome.

Chitosan/poly‐octanoic acid 2‐thiophen‐3‐yl‐ethyl ester blends as a scaffold to maintain myoblasts regeneration potential in vitro

“…Chitosan films were prepared in tissue culture plates by solvent casting, dissolving chitosan (medium molecular weight, 75–85% deacetylate, Sigma‐Aldrich) in 0.5 M acetic acid. Each film of 10 μm of thickness, contained 2.5 mg chitosan/cm 2 , as described in Lieder et al Overnight dried chitosan films were neutralized by incubating with 0.5 M NaOH for 30 minutes, followed by three washes with double distilled water. Poly(OTE) films were prepared by solvent casting poly(OTE) 0.25%w/v solution in chloroform to form films containing 0.02 mg poly(OTE)/cm 2 .…”

Antimicrobial potential of bismuth lipophilic nanoparticles embedded into chitosan-based membrane

“…Chitosan-based membranes were synthesized according to Lieder et al 30) with slight modifications. To develop a 1% chitosan-based membrane, 500 mg of chitosan (mol wt 50,000-190,000 Da based on viscosity, 75-85% deacetylated; Sigma Aldrich, St. Louis, MO, USA) was mixed with 49.75 mL of deionized water (ddH 2O) and 250 µL of 100% acetic acid and allowed to settle in the dark for 1 h at room temperature.…”

193 nm ArF laser ablation and patterning of chitosan thin films

“…We note, when energy is rapidly deposited by a laser pulse, the tensile stress evolves. In this work, we simulate an acoustic wave propagating inside chitosan using an approach as previously reported [20,22]:…”

“…In addition, chitosan has been used for the realisation of biosensors for the measurement of cholesterol levels [18] and for humidity sensors [19]. Several preparation methods are available, which include spin coating for thin film work [20,21], casting methods [22], and gravity-assisted drop-sphere techniques for producing spherical samples [22,23]. There are also many methods used to pattern chitosan such as nano-imprint lithography, ion beam milling, and laser ablation processing.…”