Solution-stirring method improves crystal quality of human triosephosphate isomerase

Adachi, Hiroaki; Niino, Ai; Kinoshita, Takayoshi; Warizaya, Masaichi; Maruki, Riyo; Takano, Kazufumi; Matsumura, Hideki; Inoue, Tsuyoshi; Murakami, Satoshi; Mori, Yusuke; Sasaki, T.

doi:10.1263/jbb.101.83

Cited by 10 publications

(13 citation statements)

References 12 publications

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“…Several examples of successful application of stirring methods are reported in the literature: the crystal quality of human triosephosphate isomerase [74], adenosine deaminase [71], Src homology 2 domain-containing protein tyrosine phosphatase substrate-1 [75] was dramatically improved in this way.…”

Section: Factors Affecting Crystallization Of Bio-macromoleculesmentioning

confidence: 99%

An Overview of Biological Macromolecule Crystallization

Krauss

Merlino

Vergara

et al. 2013

IJMS

128

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The elucidation of the three dimensional structure of biological macromolecules has provided an important contribution to our current understanding of many basic mechanisms involved in life processes. This enormous impact largely results from the ability of X-ray crystallography to provide accurate structural details at atomic resolution that are a prerequisite for a deeper insight on the way in which bio-macromolecules interact with each other to build up supramolecular nano-machines capable of performing specialized biological functions. With the advent of high-energy synchrotron sources and the development of sophisticated software to solve X-ray and neutron crystal structures of large molecules, the crystallization step has become even more the bottleneck of a successful structure determination. This review introduces the general aspects of protein crystallization, summarizes conventional and innovative crystallization methods and focuses on the new strategies utilized to improve the success rate of experiments and increase crystal diffraction quality.

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Section: Factors Affecting Crystallization Of Bio-macromoleculesmentioning

confidence: 99%

An Overview of Biological Macromolecule Crystallization

Krauss

Merlino

Vergara

et al. 2013

IJMS

128

View full text Add to dashboard Cite

show abstract

“…Many crystallization techniques have been investigated, including the batch method, vapor diffusion method [1], the counter-diffusion method [2], the microseeding method [3], crystallization in hydrogel [4][5][6][7][8][9][10], or crystallization under microgravity [11][12][13], a magnetic field [14,15], or forced solution flow [10,[16][17][18][19][20][21][22][23][24][25][26][27][28]. When using these protein crystallization techniques, controlling the solution flow is one of the most important parameters [17,18,24,29], and several groups have developed practical techniques for this purpose.…”

Section: Introductionmentioning

confidence: 99%

A crystallization technique for obtaining large protein crystals with increased mechanical stability using agarose gel combined with a stirring technique

Maruyama

Hayashi

Yoshikawa

et al. 2016

Journal of Crystal Growth

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“…Adachi and co-workers (Adachi et al, 2002(Adachi et al, , 2003(Adachi et al, , 2006Murai et al, 2008) investigated the effects of one physical factor, i.e. stirring, on protein crystallization.…”

Section: Introductionmentioning

confidence: 99%

Effect of mechanical vibration on protein crystallization

Yin

Liu

et al. 2010

J Appl Cryst

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Mechanical vibration often occurs during protein crystallization; however, it is seldom considered as one of the factors influencing the crystallization process. This paper reports an investigation of the crystallization of five proteins using various crystallization conditions in a temperature‐controlled chamber on the table of a mechanical vibrator. The results show that mechanical vibration can reduce the number of crystals and improve their optical perfection. During screening of the crystallization conditions it was found that mechanical vibration could help to obtain crystals in a highly supersaturated solution in which amorphous precipitates often normally appear. It is concluded that mechanical vibration can serve as a tool for growing optically perfect crystals or for obtaining more crystallization conditions during crystallization screening.

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Solution-stirring method improves crystal quality of human triosephosphate isomerase

Cited by 10 publications

References 12 publications

An Overview of Biological Macromolecule Crystallization

An Overview of Biological Macromolecule Crystallization

A crystallization technique for obtaining large protein crystals with increased mechanical stability using agarose gel combined with a stirring technique

Effect of mechanical vibration on protein crystallization

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